@article{MRA, author = {Mogens Claesson and Anders Elm Pedersen}, title = { In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.}, journal = {Medical Research Archives}, volume = {5}, number = {Issue 9}, year = {2017}, keywords = {}, abstract = {Enteroantigen specific B cells have previously been demonstrated to play an immunomodulatory role in experimental colitis such as the T cell transfer model of colitis and regulatory B cells are known to be important for suppressing immunopathology in human IBD. Establishing immortalized B cell hybridomas with enteroantigen specificity would be an important tool to investigate further the role of B cells in the immunopathology of experimental colitis and inflammatory bowel disease. Here, based on polyethylene glycol fusion of X63 cells and enteroantigen reactive murine CD19+ B cells, we generated enteroantigen specific B cell hybridomas with proliferative capacity and IL-10 secretion against enteroantigen. The establishment of such hybridomas might be the basis for further assessing the enteroantigen specificity of gut-derived B cells, enteroantigens relevant for experimental colitis as well as human IBD including the immunoregulatory role of such enteroantigen specific B cells.}, issn = {2375-1924}, url = {https://esmed.org/MRA/mra/article/view/1435} }