@article{MRA, author = {Qin Ma and Wei Zhang and Yu-dong Wang and Zhi-feng Tian and Zhong-rui Du and Hongfang Li}, title = { Inhibitory effects of puerarin on myometrium contraction during rat menstrual cycle and its underlying mechanisms}, journal = {Medical Research Archives}, volume = {12}, number = {9}, year = {2024}, keywords = {}, abstract = {Purpose: To observe uterine contractile patterns in various phases of the menstrual cycle, and elucidate mechanisms involved in the inhibition of puerarin on rat uterine contraction. Methods: Uterus strips of Sprague Dawley rat were suspended in tissue chambers to measure the contractile activities of the strips. Results: Isolated myometrial strips possessed three typical forms of contractions: spontaneous rhythmic contraction, tonic contraction, and irregular contraction, respectively appearing in varied phases of the rat menstrual cycle. Puerarin not only dose-dependently inhibited spontaneous contraction but also could dose-dependently inhibit KCl or PGF2α-induced uterus contractions. The inhibitory effects induced by puerarin in KCl-precontracted myometrial strips could be attenuated partly by β2 adrenoceptor antagonist ICI118551 but had no obvious changes prior to treatment with glibenclamide, Nw-L-nitro-arginine, ICI 182,780, tamoxifen or indomethacin. In high K+ Ca2+-free Kreb’s solution containing 0.01 mmol/L egtazic acid (EGTA), puerarin decreased the first phase contractions induced by oxytocin, acetylcholine, or prostaglandin F2α, but did not affect the second contractions induced by CaCl2, and also had no marked effect on the concentration-response curve of CaCl2. Conclusion: The results demonstrate that uterus contraction patterns differ during varied menstrual cycle phases. Puerarin can inhibit isolated myometrium contractions, probably due to activation of β2-adrenoceptor, and partially via reduction of Ca2+ release from the sarcoplasmic reticulum.}, issn = {2375-1924}, doi = {10.18103/mra.v12i9.5802}, url = {https://esmed.org/MRA/mra/article/view/5802} }