@article{MRA,
	author = {Eduardo Álvarez-Lozano and Ramón Quintanilla-Loredo and Adrián Torres-Gaytán and Abraham Vazquez-Salas and Francisco Forriol-Campos},
	title = { Evaluation of the Osteogenic Potential of a Three-Dimensional Bioimplant for the Treatment of Bone Defects: Innovations in bone regeneration},
	journal = {Medical Research Archives},
	volume = {13},
	number = {3},
	year = {2025},
	keywords = {},
	abstract = {Introduction: Massive bone defects (MBD) represent a significant clinical challenge due to the difficulty in achieving effective bone consolidation. This study evaluates the osteogenic potential of a three-dimensional bioimplant composed of demineralized bone matrix (DBM), collagen, hydroxyapatite (HAp), and bone marrow nucleated cells in animal models.
Methods: An experimental study was performed in 45 lambs (Ovis aries) with bone defects in the proximal tibia. Three groups were compared: (1) three-dimensional bioimplant, (2) bioimplant with autologous bone marrow nucleated cells, and (3) bone allograft. Bone regeneration was assessed by radiological, histological, histochemical, and immunohistochemical analysis.
Results: The group treated with the bioimplant, and nucleated cells showed greater osteoid formation and osteoblastic activity compared to the other groups. PAS stain positivity and the presence of osteoblasts (PTHR1+) were significantly higher in this group (p<0.001).
Conclusion: The combination of a three-dimensional bioimplant with nucleated bone marrow cells significantly improves bone regeneration compared to the use of a cell-free bioimplant or an allograft, suggesting a promising alternative for the repair of massive bone defects.},
	issn = {2375-1924},	doi = {10.18103/mra.v13i3.6214},
	url = {https://esmed.org/MRA/mra/article/view/6214}
}