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01 · ABSTRACT

Abstract

Background: Isoniazid mono-resistant tuberculosis defined by resistance to isoniazid while remaining susceptible to other first-line drugs, presents significant treatment challenges. This study aimed to determine the time to sputum culture conversion and assess the intensive phase treatment outcomes among Isoniazid mono-resistant tuberculosis patients in South Africa.

Methods: This retrospective cohort study analysed 145 Isoniazid mono-resistant tuberculosis patients aged 18–65 years treated at Brewelskoof Hospital, Western Cape, South Africa, from 2009 to 2015. Demographic and clinical characteristics were obtained from the hospital’s tuberculosis registry. Data analyses used descriptive statistics, chi-square, analysis of variance, Kaplan–Meier survival, and Cox proportional hazards models to assess the time to sputum culture conversion and the intensive phase treatment outcomes, adjusting for relevant covariates. All statistical analyses were conducted using SAS 9.4 (SAS Institute, Cary, NC, USA).

Results: The median age of Isoniazid mono-resistant tuberculosis patients was 37 years, with an interquartile range (IQR) of 30–44, with 76 (52.41%) being males. After six months of treatment, 71 patients (48.97%) achieved sputum culture conversion, while 74 (51.03%) did not. The overall median time to sputum culture conversion was 55 days (IQR: 25–91), with significant variation across age groups (p=0.0074). The shortest time to sputum culture conversion was observed among patients aged 50–59 years (33.71±7.89 days). Overall, most Isoniazid mono-resistant tuberculosis patients (72.41%, 95%CI: 64.63–79.04) experienced unfavourable intensive phase treatment outcomes. Patients with unfavourable outcomes were 79% more likely to have delayed sputum culture conversion compared to those with favourable outcomes (HRR: 1.79, 95% CI: 1.07–2.98, p=0.0264). Conversely, patients who were 50-59 years were 4 times at risk of early conversion compared to individuals <20 years of age (HRR: 4.19, 95% CI: 1.13–15.61, p=0.0327).

Conclusion: Isoniazid mono-resistant tuberculosis patients in this cohort experienced prolonged time to sputum culture conversion and a high proportion of unfavourable intensive phase treatment outcomes. Delayed sputum culture conversion was strongly associated with poor treatment outcomes, highlighting the critical role isoniazid plays in drug-resistant tuberculosis therapy. Consequently, there is need for intensified monitoring and targeted interventions to improve early culture conversion and treatment success during the intensive phase treatment.
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02 · PUBLICATION RECORD

Article details

JournalMedical Research Archives
IssueVol 14 No 1 (2026): Vol.14, Issue 1, January 2026
SectionResearch Articles
Published28 January 2026
DOI10.18103/mra.v14i1.7138
ISSN2375-1924
03 · RIGHTS & REUSE

Rights & reuse

This article is published under a Creative Commons Attribution License (CC BY 3.0) and may be shared or distributed by anyone as long as attribution is given to the journal.

Authors & affiliations

OO

Osaretin Christabel Okonji

School of Pharmacy, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.

PM

Pierre Mugabo

School of Pharmacy, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.

OM

Osaro Mgbere

2Department of Pharmaceutical Health Outcomes and Policy, College of Pharmacy, University of Houston, Houston, TX, USA; Department of Health Systems and Population Health Sciences, Tilman J. Fertitta Family College of Medicine, University of Houston, Houston, TX, USA;  Institute of Community Health, University of Houston, Houston, TX, USA; Public Health Science and Surveillance Division, Houston Health Department, Houston, TX, USA

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