Invitro Cytotoxic Effect of Methanolic Extract from Eclipta prostrata L. against Human Colorectal Adenocarcinoma Cell Line

Main Article Content

Kanagaraj C. Mohanapriya B Aravindh S Sathyapriya A Suresh S N

Abstract

Colorectal cancer is the third leading affected in men after lung and prostate cancer tumours and in women after lung and breast cancers. Current food habitation as potential risk factors for the growth of colorectal cancer include lack of physical activity, alcohol consumption, smoking, a low fiber and high fat nutrition, obesity and inadequate fruit and vegetable usage. Eclipta prostrata (L). is commonly found on roadsides and waste lands. It is annual herbaceous plant have a long history of traditional values; medicines use which is various part of the world especially in tropical, subtropical regional widely. This herbs already found the curative properties and has been utilized as analgesic, antibacterial, antihepatotoxic, antihaemorrhagic, antihyperglycemic, antioxidant, immunomodulatory properties and it is considered as a good rejuvenator too. Present study to find the in vitro cytotoxic effect of methanolic extract against the Human Colorectal Adenocarcinoma cells. Cancer cells were treated with different concentration of extract and incubated for 24hours and the cytotoxic effect was observed in microscopy and cell viability was determined by 2,5-diphenyl-2H-tetrazolium bromide assay shows half maximal inhibitory concentration shows 62.44 µg/ml. The nuclear 4’6-diamidino-2-phenylindole staining was performed and confirm the existence of apoptosis morphological changes of the cells were studied. Besides, this study to continue the identification of specific metabolites from methanolic extract of Eclipta prostrata (L.) recommended for the discovery of potential anti-proliferative and anticancer compounds.

Keywords: Eclipta prostrata (L.), Colorectal Cancer, Methanol Extract, Cytotoxicity

Article Details

How to Cite
C., Kanagaraj et al. Invitro Cytotoxic Effect of Methanolic Extract from Eclipta prostrata L. against Human Colorectal Adenocarcinoma Cell Line. Medical Research Archives, [S.l.], v. 11, n. 2, feb. 2023. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/3572>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.18103/mra.v11i2.3572.
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Research Articles

References

1. Kirtikar KR, Basu BD. Indian Medicinal Plants, Deharadun (India): International book distributors, 1999, II.
2. Indian Herbal Pharmacopoeia. A joint publication of IDMA and RRL Jammu-Tavi. 1998; I:81-85.
3. Jahan R, Al-Nahain A, Majumder S, Rahmatullah M. Ethnopharmacological Significance of Eclipta alba (L.) Hassk. (Asteraceae). Int. Sch. Res. Not. 2014. doi: 10.1155/2014/385969.
4. Dalal S, Kataria S, Sastry K, Rana SVS. Phytochemical Screening of Methanolic Extract and Antibacterial Activity of Active Principles of Hepatoprotective Herb, Eclipta alba. Ethnobot. Leafl. 2010, 3. https://opensiuc.lib.siu.edu/ebl/vol2010/iss3/3
5. Bakht J, Islam A, Ali H, Tayyab M, Shafi M. Antimicrobial Potentials of Eclipta alba by Disc Diffusion Method. Afr. J. Biotechnol. 2011, 10, 7658–7667. doi: 10.5897/AJB11.454
6. Jayathirtha MG, Mishra SH. Preliminary Immunomodulatory Activities of Methanol Extracts of Eclipta alba and Centella asiatica. Phytomedicine 2004, 11, 361–365. doi: 10.1078/0944711041495236
7. The Indian Pharmacopoeia Govt of India Publication, 2010, Volume3.
8. Yahara S, Ding N, Nohara T, Masuda K and Ageta H. Taraxastane glycosides from. Eclipta alba. Phytochem 44: 131-135, 1997. doi:10.1016/S0031-9422(96)00473-6
9. Santhosh KC, Govindasamy S and Sukumar E. Lipid lowering activity of Eclipta prostrata in experimental hyperlipidemia. J Ethnopharmacol 2006, (105) 332-335. doi: 10.1016/j.jep.2005.10.031.
10. WHO., Preventing Risks and Taking Actions, 2000-2005, Geneva, 2002
11. Bershyal S, Kanagaraj C, Aravindh S, Mohanapriya B: GCMS analysis in Methanolic Extract of Residential plant Eclipta Prostrata (L). Int.J.Curr.Res.Chem.Pharm.Sci 2021,.8(6):1-4. doi: 10.22192/ijcrcps
12. Da Rocha A B, Lopes R M, Schwartsmann G. Natural products in anticancer therapy, Current Opinion in Pharmacology, 2001, (1) 4, 364–369. doi: 10.1016/s1471-4892(01)00063-7.
13. Cragg GM, Grothaus PG, Newman DJ. Impact of natural products on developing new anti-cancer agents, Chemical Reviews, 2009, (109) 7, 3012–3043. doi: 10.1021/cr900019j.
14. Kumar S, Mishra A, Pandey AK. Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complement Altern Med. 2013;13(1):120-128. doi: 10.1186/1472-6882-13-120
15. Kntayya S, Ibrahim M, Mohd Ain N, Iori R, Ioannides C, Abdull RA. Induction of apoptosis and cytotoxicity by isothiocyanate sulforaphene in human hepatocarcinoma HepG2 Cells. Nutrients. 2018;10(6):718. https://doi.org/10.3390/nu10060718.
16. Boregowda RS, Murali N, Udayashankar AC, Niranjana SR, Lund OS, Prakash HS. Antifungal Activity of Eclipta alba Metabolites against Sorghum Pathogens. Plants 2019, 8, 72. 10.3390/plants8030072
17. Lee HY. Enhancement of Skin Anti-Inflammatory Activities of Eclipta prostrata L. from the Ultrasonic Extraction Process. Appl. Sci. 2017, 7, 1227. doi: 10.3390/app7121227
18. Kang EY, Kim HK, Jung JY, Kim JH, Woo TK, Choi JI, Kim JH, Ahn C, Lee HG, Go GW. Combined Extract of Leonurus Japonicus Houtt, Eclipta prostrata L., and Pueraria Lobata Ohwi Improved Hot Flashes and Depression in an Ovariectomized Rat Model of Menopause. Foods 2021, (10) 180. doi:10.3390/foods10010180.
19. Gautam TP. Indigenous Uses of Some Medicinal Plants in Panchthar District, Nepal. Nepal. J. Biosci. 2011, (1) 125–130. doi: 10.3126/NJBS.V1I0.7479.
20. Thakur VD and Mengi SA: Neuropharmacological profile of Eclipta alba (Linn.) Hassk. J Ethnopharmacol 2005 (102) 23-31. doi: 10.1016/j.jep.2005.05.037.
21. Ma-Ma K, Nyunt N, Tin KM. The protective effect of Eclipta alba on carbon tetrachloride-induced acute liver damage. Toxicol Appl Pharmacol. 1978 (45) 723-728. doi:10.1016/0041-008X(78)90165-5
22. Karthikumar S, Vigneswari K and Jegatheesan K, Screening of antibacterial and antioxidant activities of leaves of Eclipta prostrata (L). Sci Res Essay. 2007 (2) 101-104. doi:10.5897/SRE.9000819
23. Pakizehkar S, Ranji N, Naderi Sohi A, Sadeghizadeh M. Polymersome assisted delivery of curcumin: A suitable approach to decrease cancer stemness markers and regulate miRNAs expression in HT29 colorectal cancer cells. Polym Adv Technol. 2020, (31) 160-177. Doi:10.1002/pat.4759
24. Kumari CS, Govindasamy S and Sukumar E. Lipid lowering activity of Eclipta prostrata in experimental hyperlipidemia. J Ethnopharmacol. 2006 (105) 332-335. doi:10.1016/j.jep.2005.10.031
25. Arya RK, Singh A, Yadav NK, Cheruvu SH, Hossain Z, Meena S, Maheshwari S, Singh AK, Shahab U, Sharma C et al. Anti-Breast Tumor Activity of Eclipta Extract in-Vitro and in-Vivo: Novel Evidence of Endoplasmic Reticulum Specific Localization of Hsp60 during Apoptosis. Sci. Rep. 2015, (5) 18457. Doi:10.1038/srep18457.
26. Kim HY, Kim HM, Ryu B, Lee JS, Choi JH, Jang DS. Constituents of the Aerial Parts of Eclipta prostrata and Their Cytotoxicity on Human Ovarian Cancer Cells in Vitro. Arch. Pharm. Res. 2015, (38) 1963–1969. doi: 10.1007/s12272-015-0599-2.
27. Chaudhary H, Dhuna V, Singh J, Kamboj SS, Seshadri S. Evaluation of Hydro-Alcoholic Extract of Eclipta alba for Its Anticancer Potential: An in Vitro Study. J. Ethnopharmacol. 2011 (136) 363–367. doi: 10.1016/j.jep.2011.04.066.
28. Sawant M, Isaac JC and Narayanan S. Analgesic studies on total alkaloids and alcohol extracts of Eclipta alba (Linn.) Hassk. Phytother Res. 2004 (18) 111-113. doi: 10.1002/ptr.1165.
29. Jayathirtha MG and Mishra SH: Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine. 2004 (11), 361-365. doi: 10.1078/0944711041495236.