The objective of study is to provide an indication on features of diagnostic testing of SARS-CoV-2 by RT-PCR, including parameters of sensitivity, specificity, positive and negative likelihood ratios.
Coronavirus Disease is the fifth international emergency after 1918, Spanish flu pandemic, triggered by Severe Acute Respiratory Syndrome Coronavirus2 (SARS-CoV2). On 30 January the WHO acknowledged COVID-19 to be a global health disaster of international importance and a pandemic on 11 March 2020. In vitro analysis of the data shows that for SARS-CoV-2 the RT-PCR test is highly specific, as it is not counter react with nucleic acid of other viruses.
Oral pharyngeal and nasopharyngeal swabs were collected into a 3 ml viral transport media (VTM) and transported to Laboratory. Extraction of the viral RNA was done by Qiasymphony DSP Virus/ Pathogen mini kit (Qiagen GmbH, Germany). For amplification process of RT-PCR qualitative detection of SARS-CoV-2 RNA utilizing with SYSTAAQ 2019-Novel Coronavirus (COVID-19) Real time PCR kit using a BIORAD-CFX 96.
Out of 15,049, 3195 samples were positive for covid-19 qPCR. Ratio of Male patients was greater than female as 63.7% male and 36.3% female were affected with Covid-19. Symptom wise analysis shows 62% patient were asymptomatic, 22.7% mild, 1.7% moderate, 12.7% stable, 0.6% severe and 0.2% were critical. Our analysis reveals age group 1 (4.9%), group 2 (55.5%), group 3 (27.5%), and group 4 (12.1%) were effected with SARS-nCoV-2. Our result shows
3.0 % patients were deceased and 97% were recovered.
Our findings contribute to the evolving understanding of the sophisticated interaction between this emerging SARS-CoV-2 virus and nucleic acid based target testing of COVID-19.
Keywords: Pandemic, Covid-19, RT-PCR, RNA
Dr. Ghazala Rubi