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Home  >  Medical Research Archives  >  Issue 149  > Comparison of cytotoxicity caused by Viscum album in human mesenchymal stem cells and hepatocellular carcinoma cells
Published in the Medical Research Archives
May 2024 Issue

Comparison of cytotoxicity caused by Viscum album in human mesenchymal stem cells and hepatocellular carcinoma cells

Published on May 26, 2024

DOI 

Abstract

 

Viscum album (VA), also known as Mistletoe, has various therapeutic properties, including analgesic, anti-inflammatory, and anticancer effects. It has been used for treating different types of cancer, exhibiting proven efficacy against breast cancer, glioblastoma, carcinoma, and other advanced-stage tumor types. Besides promoting improvements in the clinical condition, VA also helps reduce the side effects caused by conventional treatment, thereby offering patients a better quality of life. Hence, the objective of this study was to assess the effects of the homeopathic dilution of VA at the potency of D30 (1x10-30) (VA D30) on human mesenchymal stem cells (MSCs) and hepatocellular carcinoma cells (HepG2). The cells were grown in 75 cm² flasks until they reached approximately 80% confluence. Subsequently, they were trypsinized and plated in 96-well plates at 10,000 cells/well. After 24 hours of incubation in an oven, VA was added at 30 and 40 µL/mL concentrations. The cells were further incubated for an additional 48 hours. After the treatment, the cells underwent a cell viability test using MTT. The results indicated a decrease in HepG2 viability, while no damage to normal cells (MSCs) was detected. The findings suggest that VAD30 holds promise as a potential therapeutic agent in treating hepatocellular carcinoma due to its observed cytotoxicity towards HepG2 cells while exhibiting no adverse effects on mesenchymal stem cells at the equivalent concentration.

Author info

Ana Valle, Aloiso De Carvalho, Samir Rahme, Andressa Araujo, Patrícia Malard, Hilana Sena Brunel

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