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Tumor cells that circulate in blood can initiate tumor metastases in distant sites. Circulating tumor cells (CTCs) are rare and heterogeneous, and display various phenotypes. The molecular characterization of each subpopulation is fundamental to its phenotypic identification and description of relevant genetic alterations for the management of patient's disease. We have previously described the development of a new mesofluidic multiplexed-immunosensor device for the capture and analysis of CTCs from blood samples of breast cancer patients. To aid sorting cancer cell subtypes for their characterization, we improve our methodology by serially interconnecting three-designed multiplexed-immunosensor devices that allowed grafting a subset of selective and specific antibodies for the capture of each cancer cell subtype. This new method gives a powerful tool to identify circulating tumor cell subtypes.