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The aim was to determine the association between R72P polymorphism of p53 gene and the risk of developing squamous intraepithelial cervical lesions in HPV-16 and /or 18 infected women. Two groups of women were included in this study: 74 patients HPV-16 and /or 18 positive with a cytological and colposcopy diagnosis of squamous intraepithelial lesion and a group of unrelated 130 healthy blood-donors. The viral genotype, allele and genotype of the polymorphism frequencies were determined by PCR approached. The results were analyzed with the statistical programs DeFinetti and STAT intercooled v11.1. Patients with high-grade squamous intrahepitelial lesions (HG-SIL) were infected mainly by HPV-16 (60.72%) compared to low-grade lesions (LG-SIL) (39.28%) (OR 3.14; p= 0.037), with HPV-18 genotype 68.96% of LG-SIL and 31.04% were HG-SIL (OR=0.24, p=0.006). HG-SIL were more common in patients carrying both viral genotypes (70.59% vs 29.41%) (OR 2.8, p = 0.008). A statistically significant association was observed between the genotype R/R and HG-SIL (OR=11.25, IC 3.8-33.29, p= 0.000) compared to those with LG-SIL. The P/R genotype was significantly more frequent in patients LG-SIL, compared to HG-SIL (OR=0.27, p=0.00). In conclusion patients with the R/R genotype showed more susceptibility to HPV-16 infection and they have almost 12 times more risk probability of HG-SIL compared to women having the heterozygous genotype and HPV-18.