Use of Gas Chromatography and of a Specific Hydrogen Sensor to Prove the Emission of Hydrogen from Bacterial Cultures of infected Food, Veterinary and Human Medical Specimens such as Lyme-Disease and to Test the Efficacy of Applied Antibiotics. Bruno Kolb*

Abstract

AbstractAuthor Info
Volatile organic and inorganic compounds emitted from bacteria and cultured in closed septum vials can be analysed by headspace gas chromatography (HS-GC) . But if only a single compound such as hydrogen is monitored, a specific hydrogen sensor is suited as well and both techniques are described and compared on the example of infected food and have also been applied for veterinary and human medicine. Lyme-disease was selected here as representative for diagnosis and therapy of diseases caused by bacterial infection. Addition of standard or natural antibiotics to the samples in the vials enables to investigate the efficacy of their antibiotic effect. The electrical output of both HS-GC and the sensor approach enables digital data processing and documentation.
Key Words
Bacteria; Hydrogen Emission; Hydrogen Sensor; Gas Chromatography; Antibiotics; Food; Lyme Disease; Diagnosis; Therapy