VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Vascular Endothelial Cells.

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Greg B. Pott Mark Tsurudome Jamie Bui Charles Banfield Sami Hourieh Harsh Pratap Marc L. Goalstone



Atherosclerosis is a cardiovascular disease that begins with inflammation and ends in decreased lumen diameter, occlusion and rupture if not addressed appropriately.  Markers of vascular inflammation are, but not limited to, increased expression of Cell Adhesion Molecules on the vascular endothelium, recruitment of serum monocytes to the endothelium, and increases in the presence of inflammatory cytokines.  Many factors can contribute to vascular inflammation and atherosclerosis, including diabetes, obesity, smoking, and genetic factors. In this report, we examine how cigarette smoke extract (CSE) contributes to the onset of vascular inflammation by (1) increasing VCAM-1 and ICAM-1 expression in endothelium in a time- and dose-dependent manner, (2) affecting cytokine expression in endothelial cells, (3) increasing monocyte adhesion to carotid endothelial cells and (4) increasing the number of monocytes that migrate across a monolayer of carotid endothelial cells.


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How to Cite
POTT, Greg B. et al. VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Vascular Endothelial Cells.. Medical Research Archives, [S.l.], v. 5, n. 7, july 2017. ISSN 2375-1924. Available at: <>. Date accessed: 28 feb. 2024.
cigarette smoke extract, monocytes, carotid endothelial cells
Research Articles


1. Hsueh WA and Law RE (1999) Insulin signaling in the arterial wall. Am J Cardiol 84: 21J-24J.

2. Prescott SM, McIntyre TM and Zimmerman GA (2001) Events at the vascular wall: The molecular basis of inflammation. J. Invest. Med. 49: 104-111.

3. Ley K, Miller YI and Hedrick CC (2011) Monocyte and macrophage dynamics during atherogenesis. Arterioscler Thromb Vasc Biol 31: 1506-1516.

4. Breen DM, Chan KK, Dhaliwall JK, Ward MR, Al Koudsi N, Lam L, De Souza M, Ghanim H, Dandona P, Stewart DJ, Bendeck MP and Giacca A (2009) Insulin increases reendothelialization and inhibits cell migration and neointimal growth after arterial injury. Arterioscler Thromb Vasc Biol 29: 1060-1066.

5. Standl E (1995) Hyperinsulinemia and atherosclerosis. Clin Invest Med 18: 261-266.

6. Endemann DH and Schiffrin EL (2004) Endothelial dysfunction. J Am Soc Nephrol 15: 1983-1992.

7. Nana-Sinkam SP, Lee JD, Sotto-Santiago S, Stearman RS, Keith RL, Choudhury Q, Cool C, Parr J, Moore MD, Bull TM, Voelkel NF and Geraci MW (2007) Prostacyclin prevents pulmonary endothelial cell apoptosis induced by cigarette smoke. Am J Respir Crit Care Med 175: 676-685.

8. Horvathova M, Jahnova E, Szabova M, Tulinska M, Kuricova M, Liskova A, Volkovova K and Dusinska M (2009) The relationship between cell surface markers, cytokines, ageing, and cigarette smoking. Bratisl Lek Listy 110: 394-400.

9. Cybulsky MI, Iiyama K, Li H, Zhu S, Chen M, Iiyama M, Davis V, Gutierrez-Ramos JC, Connelly PW and Milstone DS (2001) A major role for VCAM-1, but not ICAM-1, in early atherosclerosis. J Clin Invest 107: 1255-1262.

10. Singh RB, Mengi SA, Xu YJ, Arneja AS and Dhalla NS (2002) Pathogenesis of atherosclerosis: A multifactorial process. Exp Clin Cardiol 7: 40-53.

11. Madonna R, Massaro M and De Caterina R (2008) Insulin potentiates cytokine-induced VCAM-1 expression in human endothelial cells. Biochim Biophys Acta 1782: 511-516.

12. Scott DA and Palmer RM (2002) The influence of tobacco smoking on adhesion molecule profiles. Tob Induc Dis 1: 7-25.

13. Selya AS and Hesse ND (2017) Time to first cigarette and serum cholesterol levels. Soc Sci Med 174: 213-219.

14. Maranhao RC and Leite AC (2014) Development of Anti-Atherosclerosis Therapy Based on the Inflammatory and Proliferative Aspects of the Disease. Curr Pharm Des

15. Selvin E, Halushka MK, Rawlings AM, Hoogeveen RC, Ballantyne CM, Coresh J and Astor BC (2013) sRAGE and risk of diabetes, cardiovascular disease, and death. Diabetes 62: 2116-2121.

16. Zhu T, Zhang L, Ling S, Duan J, Qian F, Li Y and Xu JW (2014) Scropolioside B Inhibits IL-1 beta and Cytokines Expression through NF- kappa B and Inflammasome NLRP3 Pathways. Mediators Inflamm 2014: 819053.

17. Erusan RR, Nalini D, Ganesan M and Malathi R (2012) Correlation between Obesity and Inflammation in Cardiovascular Diseases - Evaluation of Leptin and Inflammatory Cytokines. Open Journal of Endocrine and Metabolic Diseases 2: 7-15.

18. Schaberg T, Rau M, Oerter R, Liebers U, Rahn W, Kaiser D, Witt C and Lode H (1996) Expression of adhesion molecules in peripheral pulmonary vessels from smokers and nonsmokers. Lung 174: 71-81.

19. Adams MR, Jessup W and Celermajer DS (1997) Cigarette smoking is associated with increased human monocyte adhesion to endothelial cells: reversibility with oral L-arginine but not vitamin C. J Am Coll Cardiol 29: 491-497.

20. Shen Y, Rattan V, Sultana C and Kalra VK (1996) Cigarette smoke condensate-induced adhesion molecule expression and transendothelial migration of monocytes. Am J Physiol 270: H1624-1633.

21. Yang GH, Li YC, Wang ZQ, Liu B, Ye W, Ni L, Zeng R, Miao SY, Wang LF and Liu CW (2014) Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury. J Pineal Res 57: 451-458.

22. Rao P, Ande A, Sinha N, Kumar A and Kumar S (2016) Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells. PLoS One 11: e0155791.