In vitro Experiments of Magnetic Field in Human Exposures of Intermediate Frequency
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Abstract
Electromagnetic safety standards are continually updated according to the latest and accumulating scientific pieces of evidence in cell studies, in order to confirm the exact impacts of Electromagnetic Field (EMF) on in-vitro cellular level. Various biological experiments, of both in-vivo and in-vitro, have been conducted for the investigation of the potential effects of EMF, however, these investigations are mostly focused either on the power frequency or microwave EM exposures aiming at the impact on osteocyte or cancer cells. This paper presents an investigation of the bio-electromagnetic impact of the cell viability to HT1080 and UR61 cells and the DNA integrity to the HT1080 cell, under an acute magnetic field of intermediate frequencies (IF) at 100 kHz. IF frequencies are commonly adopted in the wireless power transfer in E-vehicle applications in recent years; this study aims to provide the missing evidence of in-vitro experiments of Magnetic Field in Human Exposures of IF. The impact of the cell viability variation was investigated by MTT assay, while the DNA integrity was investigated by Comet assay. Three identical repeated experiments of prolonging exposure of 6-hours magnetic field of 21A/m, at 100 kHz were carried out for DNA fragmentation; results have indicated that exposure level which is the reference level for the general public of ICNIRP of 21A/m for HT1080, would barely cause any impact on DNA integrity. Three identical repeated experiments of prolonging exposure of 24-hours at the same level of exposure for cell viability investigation, results have indicated that there is a significant statistical difference of cell viability observed in both HT1080 and UR61 cells. The cell viability outcomes were concluded by t-test for a p-value of less than 0.05.
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