Abstract
In this study, the effects of vinorelbine (A microtubule inhibitor), para aminophenol derived acetaminophen and LiCl (a mood stabilizing agent) on monolayer and spheroid models of MDAH-2774 human ovarian cancer cell lines were investigated using both single and combined application of the drugs. The effects of the drugs on cell proliferation, cytotoxicity and S- phase fraction of cell cycle at 24,48 and 72 hours, respectively, were evaluated in cell culture. Evaluation of each drug in monolayer and spheroid cell culture compared to control group showed that vinorelbine, lithium and acetaminophen caused significant decrease in both cell proliferation and cell cycle progression and S-Phase of DNA synthesis. However, the effect of acetaminophen in relation to the other drugs was most pronounced.
There was no significant difference between drug combination groups and single drug groups in monolayer and spheroid cell culture. The data indicate that the effects of vinorelbine, lithium chloride and acetaminophen on monolayer and spheroid models of MDAH-2774 human ovarian cancer cell line caused a decrease in cell number in the cell cycle sythesis phase and prevented cell proliferation. We observed different size and shaps autophagic vacuols and defective mitochondria in the cytoplasm of the cells treated with vinorelbine. These findings were evaluated as a sign of autophagic cell death.