Negative Pressure Wound Therapy: Basic Science Review

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Michael J Morykwas Dominic Massary


Since the initial publication in 1997 of the basic research behind Negative Pressure Wound Therapy and the additional review in 2006, much additional research has been conducted.  This short review highlights some of this additional basic research into the mechanisms and physiological responses to the controlled applicaiton of sub-atmospheric pressure for wound care.

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MORYKWAS, Michael J; MASSARY, Dominic. Negative Pressure Wound Therapy: Basic Science Review. Medical Research Archives, [S.l.], v. 7, n. 7, july 2019. ISSN 2375-1924. Available at: <>. Date accessed: 25 oct. 2021. doi:
Review Articles


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3. Morykwas, M.J., et al., Vacuum-assisted closure: a new method for wound control and treatment: animal studies and basic foundation. Ann Plast Surg, 1997. 38(6): p. 553-62.
4. Assadian, O., et al., Bacterial growth kinetic without the influence of the immune system using vacuum-assisted closure dressing with and without negative pressure in an in vitro wound model. Int Wound J, 2010. 7(4): p. 283-9.
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12. Liu, D., et al., Virulence analysis of Staphylococcus aureus in a rabbit model of infected full-thickness wound under negative pressure wound therapy. Antonie Van Leeuwenhoek, 2018. 111(2): p. 161-170.
13. Ludolph, I., et al., Negative pressure wound treatment with computer-controlled irrigation/instillation decreases bacterial load in contaminated wounds and facilitates wound closure. Int Wound J, 2018. 15(6): p. 978-984.
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21. Sundby, Ø., et al., Intermittent negative pressure applied to the lower limb increases foot macrocirculatory and microcirculatory blood flow pulsatility in people with spinal cord injury. Spinal Cord, 2018. 56(4): p. 382-391.
22. Sundby, Ø., et al., The acute effects of lower limb intermittent negative pressure on foot macro- and microcirculation in patients with peripheral arterial disease. PLoS One, 2017. 12(6): p. e0179001.
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24. Jung, J.A., et al., Influence of Negative-Pressure Wound Therapy on Tissue Oxygenation in Diabetic Feet. Adv Skin Wound Care, 2016. 29(8): p. 364-70.
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26. Pawar, D.R.L., et al., Influence of negative pressure wound therapy on peri-prosthetic tissue vascularization and inflammation around porous titanium percutaneous devices. J Biomed Mater Res B Appl Biomater, 2019.
27. Chen, X.J., et al., Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits. Braz J Med Biol Res, 2017. 50(12): p. e5837.
28. Chen, D., et al., Circulating fibrocyte mobilization in negative pressure wound therapy. J Cell Mol Med, 2017. 21(8): p. 1513-1522.
29. Tanaka, T., et al., Negative pressure wound therapy induces early wound healing by increased and accelerated expression of vascular endothelial growth factor receptors. Eur J Plast Surg, 2016. 39: p. 247-256.
30. Yang, S.L., et al., Effect of Negative Pressure Wound Therapy on Cellular Fibronectin and Transforming Growth Factor-β1 Expression in Diabetic Foot Wounds. Foot Ankle Int, 2017. 38(8): p. 893-900.
31. Karam, R.A., et al., Effect of negative pressure wound therapy on molecular markers in diabetic foot ulcers. Gene, 2018. 667: p. 56-61.
32. Borys, S., et al., Negative pressure wound therapy in the treatment of diabetic foot ulcers may be mediated through differential gene expression. Acta Diabetol, 2019. 56(1): p. 115-120.
33. Wang, T., et al., Negative pressure wound therapy inhibits inflammation and upregulates activating transcription factor-3 and downregulates nuclear factor-κB in diabetic patients with foot ulcerations. Diabetes Metab Res Rev, 2017. 33(4).
34. Dwivedi, M.K., et al., Expression of MMP-8 in Pressure Injuries in Spinal Cord Injury Patients Managed by Negative Pressure Wound Therapy or Conventional Wound Care: A Randomized Controlled Trial. J Wound Ostomy Continence Nurs, 2017. 44(4): p. 343-349.
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