Acute impact of a single dose of Dynamine®, TeaCrine, caffeine, and their combination on systemic hemodynamics and associated measures in men and women

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Richard Bloomer Matthew Butawan Jacquelyn Pence


Background: Dietary supplements purported to improve focus and “energy” often contain stimulants, which elevate both heart rate (HR) and blood pressure (BP). We evaluated the safety profile of the purine alkaloid methylliberine (Dynamine®) with regards to resting HR, BP, respiratory rate (RR), and body temperature (BT).

Methods: 6 men (aged: 24±4) and 6 women (aged: 22±3) ingested methylliberine (Dynamine®; Compound Solutions, Inc.) at 25mg (M25) and 100mg (M100), caffeine at 150mg (C150), M100+C150, M100 + theacrine (Teacrine®, Compound Solutions, Inc.) at 50mg (M100+T50), and M100+T50+C150. All conditions were assigned using a Latin square design, with approximately one week separating the six different assignments. HR, BP, RR, and BT were collected at baseline and 0.25, 0.5, 1, 1.5, 2, 4, 6, 8, 24, and 48 hours post-dose. Subjective mood was also recorded. Meal replacement bars and shakes were provided after hours 3 and 6.

Results: For SBP and DBP, condition effects were noted (p<0.05), with all three caffeine conditions higher than those without caffeine. Condition effects were noted for attentive (p=0.02) and energetic (p=0.02), with M100+C150 greater than M100+T50; and moody (p<0.01), with M100 and M100+T50 greater than M100+C150. Main effects of time were noted for HR (p<0.001), BT (p<0.01), SBP (p<0.01), DBP (p<0.01), and focused (p=0.02), but no statistically significant interactions were noted.

Conclusion: Methylliberine, alone or in combination with caffeine and/or theacrine, does not result in any significant increase in HR, BP, RR, or BT in healthy adults and has little impact on subjective mood. These findings should be considered with the understanding that subjects began testing in the early morning hours while in a fasted and rested state and were provided with two meals (hours 3 and 6) during the evaluation period, which may have impacted subjective mood.

Keywords: methylliberine, theacrine, caffeine, blood pressure, heart rate

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How to Cite
BLOOMER, Richard; BUTAWAN, Matthew; PENCE, Jacquelyn. Acute impact of a single dose of Dynamine®, TeaCrine, caffeine, and their combination on systemic hemodynamics and associated measures in men and women. Medical Research Archives, [S.l.], v. 8, n. 4, apr. 2020. ISSN 2375-1924. Available at: <>. Date accessed: 14 june 2024. doi:
Research Articles


1. Zheng X-Q, Ye C-X, Kato M, Crozier A, Ashihara H. Theacrine (1,3,7,9-tetramethyluric acid) synthesis in leaves of a Chinese tea, kucha (Camellia assamica var. kucha). Phytochemistry. 2002;60(2):129-134. doi:10.1016/S0031-9422(02)00086-9
2. Ashihara H, Kato M, Crozier A. Distribution, Biosynthesis and Catabolism of Methylxanthines in Plants. In: Methylxanthines. Vol 200. Berlin, Heidelberg: Springer Berlin Heidelberg; 2011:11-31. doi:10.1007/978-3-642-13443-2_2
3. Maier HG, Weidner M. Minor alkaloids in caffeine containing stimulants. Deutsche Lebensmittel-Rundschau. 2000;96(10):363-368.
4. Petermann JB, Baumann TW. Metabolic Relations between Methylxanthines and Methyluric Acids in Coffea L. Plant Physiol. 1983;73(4):961-964. doi:10.1104/pp.73.4.961
5. Taylor L, Mumford P, Roberts M, et al. Safety of TeaCrine®, a non-habituating, naturally-occurring purine alkaloid over eight weeks of continuous use. J Int Soc Sports Nutr. 2016;13(1):2. doi:10.1186/s12970-016-0113-3
6. Kuhman D, Joyner K, Bloomer R. Cognitive Performance and Mood Following Ingestion of a Theacrine-Containing Dietary Supplement, Caffeine, or Placebo by Young Men and Women. Nutrients. 2015;7(11):9618-9632. doi:10.3390/nu7115484
7. Ziegenfuss TN, Habowski SM, Sandrock JE, Kedia AW, Kerksick CM, Lopez HL. A Two-Part Approach to Examine the Effects of Theacrine (TeaCrine®) Supplementation on Oxygen Consumption, Hemodynamic Responses, and Subjective Measures of Cognitive and Psychometric Parameters. Journal of Dietary Supplements. 2017;14(1):9-24. doi:10.1080/19390211.2016.1178678
8. Murbach TS, Glávits R, Endres JR, et al. A Toxicological Evaluation of Methylliberine (Dynamine®). Journal of Toxicology. 2019;2019:1-25. doi:10.1155/2019/4981420
9. Nawrot P, Jordan S, Eastwood J, Rotstein J, Hugenholtz A, Feeley M. Effects of caffeine on human health. Food Additives and Contaminants. 2003;20(1):1-30. doi:10.1080/0265203021000007840
10. Passmore AP, Kondowe GB, Johnston GD. Renal and cardiovascular effects of caffeine: A dose–response study. Clinical Science. 1987;72(6):749-756. doi:10.1042/cs0720749
11. Quinlan PT, Lane J, Moore KL, Aspen J, Rycroft JA, O’Brien DC. The Acute Physiological and Mood Effects of Tea and Coffee. Pharmacology Biochemistry and Behavior. 2000;66(1):19-28. doi:10.1016/S0091-3057(00)00192-1
12. Butawan M, Stockton MB, and Bloomer RJ. Effects of a single dose of Teacrine, caffeine, or their combination on subjective feelings, cognitive performance, and hemodynamics in men and women. Poster presented at 14th Annual Conference of the International Society of Sport Nutrition; June 22-24, 2017; Phoenix, AZ.
13. He H, Ma D, Crone LB, et al. Assessment of the Drug–Drug Interaction Potential Between Theacrine and Caffeine in Humans. Journal of Caffeine Research. 2017;7(3):95-102. doi:10.1089/jcr.2017.0006
14. Stratton M, Holmes A, et al. A72. Effect of Dynamine™ with and without TeaCrine® over four weeks of continuous use on cardiovascular function, and psychometric parameters: a pilot study. J Int Soc Sports Nutr. 2018;15(256) doi: 10.1186/s12970-018-0256-5
15. Gurley BJ, Steelman SC, Thomas SL. Multi-ingredient, Caffeine-containing Dietary Supplements: History, Safety, and Efficacy. Clinical Therapeutics. 2015;37(2):275-301. doi:10.1016/j.clinthera.2014.08.012
16. Jagim A, Harty P, Camic C. Common Ingredient Profiles of Multi-Ingredient Pre-Workout Supplements. Nutrients. 2019;11(2):254. doi:10.3390/nu11020254
17. Eudy AE, Gordon LL, Hockaday BC, et al. Efficacy and safety of ingredients found in preworkout supplements. American Journal of Health-System Pharmacy. 2013;70(7):577-588. doi:10.2146/ajhp120118
18. Jagim AR, Camic CL, Harty PS. Common Habits, Adverse Events, and Opinions Regarding Pre-Workout Supplement Use Among Regular Consumers. Nutrients. 2019;11(4):855. doi:10.3390/nu11040855
19. Boulenger J-P. Increased Sensitivity to Caffeine in Patients With Panic Disorders: Preliminary Evidence. Arch Gen Psychiatry. 1984;41(11):1067. doi:10.1001/archpsyc.1983.01790220057009
20. Cheung M, Quach J, Chan A, Nguyen NN, Shah SA. Effects of Energy Shots on Blood Pressure in Caffeine-Naive Versus Caffeine-Consuming Healthy Volunteers. Journal of Caffeine Research. 2016;6(4):148-153. doi:10.1089/jcr.2016.0013
21. Mesas AE, Leon-Muñoz LM, Rodriguez-Artalejo F, Lopez-Garcia E. The effect of coffee on blood pressure and cardiovascular disease in hypertensive individuals: a systematic review and meta-analysis. The American Journal of Clinical Nutrition. 2011;94(4):1113-1126. doi:10.3945/ajcn.111.016667
22. Svatikova A, Covassin N, Somers K, et al. Potentiated blood pressure responses to energy drink intake in caffeine naïve healthy adults: a double blind randomized controlled study. Journal of the American College of Cardiology. 2015;65(10):A1432. doi:10.1016/S0735-1097(15)61432-2