Non-invasive characterization of different types of basal cell carcinomas (BCCs) using vibrational optical coherence tomography (VOCT): Can early lesions as small as 0.05 mm be identified by VOCT?

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Frederick H. Silver Tanmay Deshmukh Nicole Ryan Arielle Romm Hari Nadiminti

Abstract

Vibrational optical coherence tomography (VOCT) has been used to non-invasively measure the resonant frequency and elastic modulus of different types of BCCs to compare the physical biomarker characteristics of each of these lesions. The results suggest that in very small lesions (about 0.05 mm in diameter) new 80Hz and 130Hz resonant frequency peaks are seen not present in normal skin or in healing wounds. In all other BCCs, new 80Hz, 130Hz and 260Hz resonant frequency peaks are found like those found in other carcinomas including SCC and melanoma.


Small BCCS are characterized by new 80Hz and 130Hz in the absence of a significant 50Hz peak unlike actinic keratoses that are characterized by 50Hz, 80Hz and 130Hz peaks. In the absence of the 260Hz peak, small BCCs appear to be a precursor to larger BCCs. Pigmented BCCs exhibit a larger ratio of the 50Hz/80Hz peaks compared to the other BCC types in addition to peaks at 130Hz and 260Hz suggesting that benign melanocyte lesions contribute to the 50 Hz peak. Further studies are needed to understand the factors that drive differences in shape and invasiveness of cancerous BCCs.


While all BCCs were found to contain new cell and blood vessel resonant frequencies that coincide with fibrotic tissue encapsulating the tumors in the papillary dermis, other factors must drive differences in the shape and invasiveness of the different BCCs. It is hypothesized that the new cells and blood vessels formed lead to the deposition of fibrous tissue in all BCCs and is partially driven by an epithelial-mesenchyme transition. It is concluded that fibrotic tissue is found encapsulating all cancerous BCCs and that this layer of tissue may limit invasiveness and metastatic behavior of this tumor type. In general, tumor shape and invasiveness are likely influenced by the exact cellular mutations in each lesion type and the extent of UV light damage experienced by the surrounding extracellular matrix.

Article Details

How to Cite
SILVER, Frederick H. et al. Non-invasive characterization of different types of basal cell carcinomas (BCCs) using vibrational optical coherence tomography (VOCT): Can early lesions as small as 0.05 mm be identified by VOCT?. Medical Research Archives, [S.l.], v. 10, n. 2, feb. 2022. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/2698>. Date accessed: 06 dec. 2022. doi: https://doi.org/10.18103/mra.v10i2.2698.
Section
Research Articles

References

1. Skin Cancer 101. Skin cancers. Skin Cancer Found J 2019;XXX VII:24-25.
2. Siegel RL, Miller KD, Fuchs HE, Jemal A. Cancer Statistics. CA. Cancer J. Clin. 2021; 71: 7–33.
3. Lomas A, Leonardi-Bee J, Bath-Hextall F. A systematic review of
worldwide incidence of nonmelanoma skin cancer. Br. J. Dermatol.
2012; 166:1069-1080
4. Amercian Academy of Dermatology Association Website. Types of skin cancer. cancer-facts-and-figures-2021.pdf.
5. Wang DM, Morgan F. Besaw RJ, Schmults CD. An ecological study of skin biopsies and skin cancer treatment procedures in the United States Medicare population.
2000 to 2015. J Amer Acad Dermatol. 2018; 78: 47–53.
6. Asgari MM, Moffet HH, Ray T, et al. Trends in basal cell carcinoma incidence and identification of high-risk subgroups. J Amer Acad Dermatol. 2015; 151: 976-981.
7. Cojocora A, Marinescu EA, Llinoui NC, Negria A, Ciurea NE. Basal Cell Carcinoma and its Impact on Different Anatomical Regions. Curr Health Sci J. 2021; 47: 75–83.
8. Jee BA, Lim H, Kwon SM, Jo Y, Park MC, Lee IJ, Woo HG. Molecular classification of basal cell carcinoma of skin by gene expression profiling. Mol Carcinog. 2015; 54:1605–1612.
9. Emiroglu N, Cengiz FP, Kemerliz F. The relationship between dermoscopy
and histopathology of basal cell carcinoma. An. Bras. Dermatol. 2015; 90: 351-356.
10. Dourmishev LA, Rusinova D, Botev I. Clinical variants, stages, and management of basal cell carcinoma. Indian Dermatol Online J 2013; 4:12-7.
11. Lupu M, Caruntu C, Popa MI, et al. Vascular patterns in basal cell
carcinoma: Dermoscopic, confocal and histopathological perspectives. Oncol. Lett. 2019; 17:4112-4125.
12. Schuh S, Holmes J, Ulrich M, et al. Imaging blood vessel morphology
in skin: dynamic optical coherence tomography as a novel potential
diagnostic tool in dermatology. Derm Ther (Heidelb). 2017; 7:187-202.
13. Paolino G, Donati M, Didona D, Mercuri SR, Cantisani C. Histology of Non-Melanoma Skin Cancers: An Update. I Biomedicines 2017: 71; doi:10.3390/biomedicines5040071.D
14. Rohani P, Yaroslavsky AN, Feng X, Jermain P, Shaath T, Neel VA. Collagen disruption as a marker for basal cell carcinoma in presurgical margin detection. Lasers Surg Med. 2018; 50:902-907.
15. Silver FH, Deshmukh T, Benedetto D, Kelkar N. Mechano-vibrational spectroscopy of skin: Are changes in collagen and vascular tissue components early signs of basal cell carcinoma formation? Skin. Res. Technol. 2020; 27:227–233.
16. Silver FH, Kelkar N, Deshmukh T, Ritter K, Ryan N, Nadiminiti H. Characterization of the biomechanical properties of skin using vibrational optical coherence tomography: Do changes in the biomechanical properties of skin stroma reflect structural changes in the extracellular matrix of cancerous lesions? Biomolecules 2021; 11: 1712. https://doi.org/10.3390/ biom11111712.
17. Silver FH, Deshmukh T, Kelkar N , Ritter N, Nicole Ryan, and Nadiminti N. The “Virtual Biopsy” of Cancerous Lesions in 3D: Non-Invasive Differentiation between Melanoma and Other Lesions Using Vibrational Optical Coherence Tomography. Dermatopathology 2021; 8:539–551. https://doi.org/ 10.3390/dermatopathology8040058.
18. Silver FH, Shah RG, Richard M, Benedetto D. Comparative “virtual biopsies” of normal skin and skin lesions using vibrational optical coherence tomography. Skin. Res. Technol. 2019; 25:743–749.
19. Silver FH, Shah RG, Richard M, Benedetto D. Use of Vibrational Optical Coherence Tomography to Image and Characterize a Squamous Cell Carcinoma. J. Dermatology Res. Ther. 2019; 5: 1–8.
20. Silver FH. Measurement of Mechanical Properties of Natural and Engineered Implants. Adv. Tissue Eng. Regen. Med. Open Access 2016; 1: 20–25.
21. Shah, R.G.; Devore, D.; Pierce, M.C.; and Silver, F.H.; Vibrational analysis of implants and tissues: Calibration and mechanical spectroscopy of multi-component materials. J. Biomed. Mater. Res. - Part A 2017, 105, 1666–1671.
22. Silver, F.H.; Kelkar, N.; Desmukh, T.; Horvath, I.; Shah, R.G.; Mechano-Vibrational Spectroscopy of Tissues and Materials Using Vibrational Optical Coherence Tomography: A New Non-Invasive and Non-Destructive Technique. Recent Progress in Materials 2020, 2, doi:10.21926/rpm.2002010.
23. Shah, R.G.; Silver, F.H.; Viscoelastic Behavior of Tissues and Implant Materials : Estimation of the Elastic Modulus and Viscous Contribution Using Optical Coherence Tomography and Vibrational Analysis. J. Biomed. Technol. Res. 2017, 3, 1–5.
24. Barak V, Goike H, Panaretakis KW, Einarsson R: Clinical utility of cytokeratins as tumor markers. Clin Biochem 2004, 37:529 –540
25. Klymkowsky MW, Savagner P. Epithelial-Mesenchymal Transition A Cancer Researcher’s Conceptual Friend and Foe. Am J Pathol 2009, 174:1588 –1593; DOI: 10.2353/ajpath.2009.080545)