Cell Viability Assessment by MTT After Treatment of Hepatocellular Carcinoma Cells (HepG2) with Chelidonium majus
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Abstract
Hepatocellular carcinoma is a type of cancer with a high mortality rate since it is primarily diagnosed in an advanced stage. Therefore, searching for therapies that help in this treatment becomes increasingly important. Therapies comprising Complementary Medicine have become more popular and have shown beneficial effects in treating various types of cancer. Homeopathy, a therapy established for more than 200 years to treat various diseases, has become the target of several studies, especially regarding chronic diseases, which are difficult to control by conventional medicine. This study evaluated the effect of the homeopathic dilution of Chelidonium majus D35 (1x10-35) in hepatocellular carcinoma cells (HepG2). Cells were cultivated in an oven at 37 ºC, 5% CO2, and then trypsinized and plated in 96-well plates for product addition. Chelidonium majus D35 was added in triplicate to each well at three different concentrations. The concentrations tested were 20, 40, and 60 µL/mL. After 48 hours of incubation with the product, cell viability was measured by MTT, and it was possible to observe a decrease in the groups that received the treatment compared to the control.
A decrease in cell viability was recorded compared to the control of cells without treatment, indicating a cytotoxic effect of Chelidonium majus D35 on tumor cells. Different studies confirm this result due to the evaluation of biologically active compounds present in extracts of Chelidonium majus D35 that control cell proliferation and induce apoptosis. The results of the in vitro tests performed were satisfactory.
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References
2. Tümen, D., Heumann, P., Gülow, K., Demirci, C.-N., Cosma, L.-S., Müller, M., & Kandulski, A. Pathogenesis and Current Treatment Strategies of Hepatocellular Carcinoma. Biomedicines, 2022: 10(12), 3202. doi: https://doi.org/10.3390/biomedicines10123202
3. Llovet, J. M., Kelley, R. K., Villanueva, A., Singal, A. G., Pikarsky, E., Roayaie, S., Lencioni, R., Koike, K., Zucman-Rossi, J., & Finn, R. S. Hepatocellular carcinoma. Nature Reviews Disease Primers, 2021: 7(1), 6. https://doi.org/10.1038/s41572-020-00240-3
4. Millward, J., McKay, K., Holmes, J. T., & Owens, C. T. Pharmacist Knowledge and Perceptions of Homeopathy: A Survey of Recent Pharmacy Graduates in Practice. Pharmacy, 2022: 10(5), 130. doi: https://doi.org/10.3390/pharmacy10050130
5. Wagenknecht, A., Dörfler, J., Freuding, M., Josfeld, L., & Huebner, J. Homeopathy effects in patients during oncological treatment: a systematic review. Journal of Cancer Research and Clinical Oncology, 2023: 149(5), 1785–1810. https://doi.org/10.1007/s00432-022-04054-6
6. Träger-Maury, S., Tournigand, C., Maindrault-Goebel, F., Afchain, P., de Gramont, A., Garcia-Larnicol, M.-L., Gervais, H., & Louvet, C. [Use of complementary medicine by cancer patients in a French oncology department]. Bulletin Du Cancer, 2007: 94(11), 1017–1025.
7. Kang, K., Jiang, H., Zhang, S., & Cheng, B. Antitumor Effects of Chelerythrine: A Literature Review. Natural Product Communications, 2022: 17(6), 1934578X2211030. doi: https://doi.org/10.1177/1934578X221103028
8. Kang, K., Jiang, H., Zhang, S., & Cheng, B. Antitumor Effects of Chelerythrine: A Literature Review. Natural Product Communications, 2022: 17(6), 1934578X221103028. doi: 10.1177/1934578X221103028
9. Nawaz, A., Arif, A., Jamal, A., Shahid, M. N., Nomani, I., & Bahwerth, F. S. Medicinal plants show remarkable antiproliferative potential in human cancer cell lines. Bioscience, Biotechnology, and Biochemistry, 2022: 86(3), 362–367. doi: https://doi.org/10.1093/bbb/zbab225
10. Shen, L., Lee, S., Joo, J. C., Hong, E., Cui, Z. Y., Jo, E., Park, S. J., & Jang, H.-J. Chelidonium majus Induces Apoptosis of Human Ovarian Cancer Cells via ATF3-Mediated Regulation of Foxo3a by Tip60. Journal of Microbiology and Biotechnology, 2022: 32(4), 493–503. doi: https://doi.org/10.4014/jmb.2109.09030
11. Lin, Y., Zhang, Q., Xie, B., Jiang, H., Shen, J., Tang, S., & Dai, C. Chelerythrine-Induced Apoptotic Cell Death in HepG2 Cells Involves the Inhibition of Akt Pathway and the Activation of Oxidative Stress and Mitochondrial Apoptotic Pathway. Antioxidants, 2022: 11(9), 1837. doi: https://doi.org/10.3390/antiox11091837
12. Warowicka, A., Popenda, Ł., Bartkowiak, G., Musidlak, O., Litowczenko-Cybulska, J., Kuźma, D., Nawrot, R., Jurga, S., & Goździcka-Józefiak, A. Protoberberine compounds extracted from Chelidonium majus L. as novel natural photosensitizers for cancer therapy. Phytomedicine, 2019: 64, 152919. doi: https://doi.org/10.1016/j.phymed.2019.152919
13. Och, A., Zalewski, D., Komsta, Ł., Kołodziej, P., Kocki, J., & Bogucka-Kocka, A. Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines. Toxins, 2019: 11(9), 485. doi: https://doi.org/10.3390/toxins11090485
14. PARK, S.-W., KIM, S. R., KIM, Y., LEE, J.-H., WOO, H.-J., YOON, Y.-K., & KIM, Y. il. Chelidonium majus L. extract induces apoptosis through caspase activity via MAPK-independent NF-κB signaling in human epidermoid carcinoma A431 cells. Oncology Reports, 2015: 33(1), 419–424. doi: https://doi.org/10.3892/or.2014.3566
15. Han, J. M., Song, H. Y., Kim, K. il, Park, W. Y., Park, S. H., Byun, E. B., & Byun, E. H. Polysaccharides from Annona muricata leaves protect against cisplatin-induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction. Molecular Medicine Reports, 2023: 27(1). doi: https://doi.org/10.3892/mmr.2022.12903