Main Article Content
With SARS-CoV-2 pandemic, a great amount of samples have been received on the different laboratories for diagnosis, sometimes in amounts difficult to handle in time, with the associated stability and quality risks.
So this study analyses the possibility to use Guthrie cards of cellulose for the storage of nasopharyngeal samples from patients with a respiratory virus infection.
With this aim, a total of 70 samples obtained on the classic UTM tube for sample collection from different patients were replicated on Guthrie cards; then, genome extraction and amplification for different viruses was performed and compare to stablish detection when samples are recovered from the cards.
Results show detection percentages of 66% for SARS-CoV-2, 75% for Picornavirus, 100% for Respiratory Syncytial Virus and 50% for Adenovirus after one week of storage.
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
2. Web page of the WHO (World Health Organisation) https://www.who.int/europe/emergencies/situations/covid-19 (consulted on 23/06/2023).
3. Ritchie H, Mathieu E, Rodés-Guirao L, Appel C, Giattino C, Ortiz-Ospina E, Hasell J, Macdonald B, Beltekian D, Roser M. Coronavirus Pandemic (COVID-19). OurWorldInData.org. [On-line]. Available at: https://ourworldindata.org/covid-cases. (consulted on 23/06/2023).
4. Manohar C, Sun J, Schlag P, et al. Agile design and development of a high throughput cobas SARS-CoV-2 RT-PCR diagnostic test. Heliyon. 2022;8(9):e10591. Published 2022 Sep 11. doi:10.1016/j.heliyon.2022.e10591
5. Hosokawa-Muto J, Fujinami Y, Mizuno N. Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics. J Forensic Leg Med. 2015;34:29-33. doi:10.1016/j.jflm.2015.04.019
6. Guthrie R, Whitney S. Phenylketonuria; detection in the newborn infant as a routine hospital procedure, State University of New York, Buffalo, 1964 (Children's Bureau Publication No. 419, 1964)
7. Comeau AM, Hsu HW, Schwerzler M, et al. Identifying human immunodeficiency virus infection at birth: application of polymerase chain reaction to Guthrie cards. J Pediatr. 1993 Aug;123(2):252-8. doi: 10.1016/s0022-3476(05)81697-x.
8. Barbi M, Binda S, Primache V, et al. Use of Guthrie cards for the early diagnosis of neonatal herpes simplex virus disease. Pediatr Infect Dis J. 1998 Mar;17(3):251-2. doi: 10.1097/00006454-199803000-00017.
9. Honkaniemi E, Talekar G, Huang W, et al. Adenovirus DNA in Guthrie cards from children who develop acute lymphoblastic leukaemia (ALL). Br J Cancer. 2010 Mar 2;102(5):796-8. doi: 10.1038/sj.bjc.6605581.
10. Pellegrinelli L, Alberti L, Pariani E, Barbi M, Binda S. Diagnosing congenital Cytomegalovirus infection: don't get rid of dried blood spots. BMC Infect Dis. 2020 Mar 12;20(1):217. doi: 10.1186/s12879-020-4941-z.
11. Martín G, Rojo-Alba S, Castelló-Abietar C, et al. Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic, Journal of Virological Methods (2021), doi:10.1016/j.jviromet.2021.114415
12. Álvarez-Argüelles, M.E., Rojo-Alba, S.; Martín, G. et al. Utilization of Microwaves: A Novel Approach to SARS-CoV-2 Genome Extraction. Appl. Microbiol. 2022, 2, 800–804. doi:10.3390/applmicrobiol2040060
13. Web page of the CDC (Centre for Diseases Prevention and Control) https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html (consulted on 23/06/2023).
14. McEwen JE, Reilly PR. Stored Guthrie cards as DNA "banks". Am J Hum Genet. 1994;55(1):196-200.
15. Erdal NB, Hakkarainen M. Degradation of Cellulose Derivatives in Laboratory, Man-Made, and Natural Environments. Biomacromolecules. 2022;23(7):2713-2729. doi:10.1021/acs.biomac.2c00336
16. American Society for Microbiology. Statemente. ASM Expresses Concern about Test Reagent Shortages. [On-line]. Available at: https://asm.org/Articles/Policy/2020/March/ASM-Expresses-Concern-about-Test-Reagent-Shortages. (Consulted on 23/06/2023).