Guthrie Cards: Exploring Their Use for Nasopharyngeal Sample Storage

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Gabriel Martin Ana Isabel Fidalgo Roberto Prada Reyes Carvajal Virginia Linares-Fernández Carmen González-Nieto María José Ferrero Rainer Campo-Ramos Isabel Cos tales

Abstract

With SARS-CoV-2 pandemic, a great amount of samples have been received on the different laboratories for diagnosis, sometimes in amounts difficult to handle in time, with the associated stability and quality risks.


So this study analyses the possibility to use Guthrie cards of cellulose for the storage of nasopharyngeal samples from patients with a respiratory virus infection.


With this aim, a total of 70 samples obtained on the classic UTM tube for sample collection from different patients were replicated on Guthrie cards; then, genome extraction and amplification for different viruses was performed and compare to stablish detection when samples are recovered from the cards.


Results show detection percentages of 66% for SARS-CoV-2, 75% for Picornavirus, 100% for Respiratory Syncytial Virus and 50% for Adenovirus after one week of storage.



Article Details

How to Cite
MARTIN, Gabriel et al. Guthrie Cards: Exploring Their Use for Nasopharyngeal Sample Storage. Medical Research Archives, [S.l.], v. 11, n. 8, aug. 2023. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/4111>. Date accessed: 21 july 2024. doi: https://doi.org/10.18103/mra.v11i8.4111.
Section
Research Articles

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