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01 · ABSTRACT

Abstract

With SARS-CoV-2 pandemic, a great amount of samples have been received on the different laboratories for diagnosis, sometimes in amounts difficult to handle in time, with the associated stability and quality risks.

So this study analyses the possibility to use Guthrie cards of cellulose for the storage of nasopharyngeal samples from patients with a respiratory virus infection.

With this aim, a total of 70 samples obtained on the classic UTM tube for sample collection from different patients were replicated on Guthrie cards; then, genome extraction and amplification for different viruses was performed and compare to stablish detection when samples are recovered from the cards.

Results show detection percentages of 66% for SARS-CoV-2, 75% for Picornavirus, 100% for Respiratory Syncytial Virus and 50% for Adenovirus after one week of storage.


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02 · PUBLICATION RECORD

Article details

JournalMedical Research Archives
IssueVol 11 No 8 (2023): August Issue, Vol.11, Issue 8
SectionResearch Articles
Published29 August 2023
DOI10.18103/mra.v11i8.4111
ISSN2375-1924
03 · RIGHTS & REUSE

Rights & reuse

This article is published under a Creative Commons Attribution License (CC BY 3.0) and may be shared or distributed by anyone as long as attribution is given to the journal.

Authors & affiliations

GM

Gabriel Martin

Grupo de Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (Oviedo, Asturias; Spain): Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

AF

Ana Isabel Fidalgo

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

RP

Roberto Prada

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

RC

Reyes Carvajal

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

VL

Virginia Linares-Fernández

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

CG

Carmen González-Nieto

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

MF

María José Ferrero

Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

RC

Rainer Campo-Ramos

Grupo de Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (Oviedo, Asturias; Spain): Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

IC

Isabel Cos tales

Grupo de Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (Oviedo, Asturias; Spain): Servicio de Microbiología, Hospital Universitario Central de Asturias (Oviedo, Asturias; Spain)

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