Effect of expansion of Shine-Dalgarno sequence for overexpression of nicotinamide adenine dinucleotide oxidase gene from Deinococcus geothermalis in Escherichia coli

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Published Jul 29, 2023
DOI: https://doi.org/10.18103/mra.v11i7.2.4172

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Main Article Content

Masahide Ishikawa
Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan.

Abstract

Thermostable enzymes have applications in biosensors and bioreactors. Overexpression of thermostable enzyme-encoding genes from thermophiles in Escherichia coli is necessary to produce sufficient quantities of such enzymes. Deinococcus gerthermalis is a moderately thermophilic bacterium and has moderately thermostable enzymes. Nicotinamide adenine dinucleotide (NADH) is an important coenzyme for various dehydrogenases. NADH oxidase (Nox) catalyze the oxidation of NADH. Therefore, Nox is a versatile enzyme that can be used in combination with many types of biosensors. This study reveals the effect of expansion of the SD sequence in the leader sequence of NADH oxidase gene from D. geothermalis for overexpression in E. coli, and shows that while 1- or 2-bases expansion of the Shine-Dalgarno sequence is effective. This study should be contributed to the expression of many genes in E. coli.

Keywords: nicotinamide adenine dinucleotide oxidase gene, Deinococcus geothermalis, Escherichia coli, E. coli

Article Details

How to Cite
ISHIKAWA, Masahide. Effect of expansion of Shine-Dalgarno sequence for overexpression of nicotinamide adenine dinucleotide oxidase gene from Deinococcus geothermalis in Escherichia coli. Medical Research Archives, [S.l.], v. 11, n. 7.2, july 2023. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/4172>. Date accessed: 21 nov. 2024. doi: https://doi.org/10.18103/mra.v11i7.2.4172.
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Issue
Vol 11 No 7.2 (2023): July Issue, Vol.11, Issue 7.2
Section
Research Articles

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