Contraction blocking during wound healing of three injured organs leads to absence of scar and induction of regeneration
Main Article Content
Abstract
We report the central role of mechanical contraction in healing of wounds in animal models, as well as in humans. Our evidence has been collected from studies of excised skin wounds as well as with the transected peripheral neve and the excised conjunctiva. We have observed that, following blocking of contraction, wounds in our animal models close by regeneration of the injured tissues. We review the mechanism of contraction blocking and tissue regeneration when wounds are grafted with a highly porous Type I collagen scaffold, named dermis regeneration template (DRT) which is required for inducing regeneration.
Keywords:
Contraction blocking, wound healing
Article Details
How to Cite
YANNAS, Ioannis V..
Contraction blocking during wound healing of three injured organs leads to absence of scar and induction of regeneration.
Medical Research Archives, [S.l.], v. 11, n. 9, oct. 2023.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/4471>. Date accessed: 17 nov. 2024.
doi: https://doi.org/10.18103/mra.v11i9.4471.
Section
Research Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
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2. Yannas I.V., Tzeranis D.S., Mammals fail to regenerate organs when wound contraction drives scar formation. npj Regen Med 6, 39 (2021)
3. Hinz B, McCulloch C. A, Coelho N. M., Mechanical regulation of myofibroblast phenoconversion and collagen contraction. Exp Cell Res 379, 119-128 (2019).
4. Birk D. E., Trelstad R. L., “Fibroblasts compartmentalize the extracellular space to regulate and facilitate collagen fibril, bundle, and macro-aggregate formation”. In A. H. Reddi, Ed., Extracellular matrix: Structure and function. (Alan R. Liss. 1985).
5. Ferdman, A. G., Yannas I.V., Scattering of light from histologic sections: A new method for the analysis of connective tissue. J Invest Dermatol, 100, 710–716 (1993).
6. Yannas I. V., Lee, E., Orgill D. P., Skrabut E.M., Murphy G.F., Synthesis and characterization of a model extracellular matrix which induces partial regenera¬tion of adult mammalian skin. Proc Nat. Acad Sci USA 86, 933–937 (1989).
7. Tzeranis, D. S., Soller, E. C., Buydash, M. C., So, P. T., Yannas I. V., In situ quantification of surface chemistry in porous collagen biomaterials. Ann Biomed Eng 44, 803–815 (2016).
8. Burke, J. F., Yannas, I. V., Quinby, W. C., Jr., Bondoc, C. C., & Jung, W. K., Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Annals of Surgery, 194, 413–428 (1981).
9. Burke, J. F., Bondoc, C. C., & Quinby, W. C., Primary burn excision and immediate grafting: A method of shortening illness. The Journal of Trauma, 14, 389–395 (1974).
10. Heimbach, D., Luterman, A., Burke, J., Cram, A., Herndon, D., Hunt, J., Jordan, M., McManus, W., Solem, L., Warden, G., et al., Artificial dermis for major burns. Annals of Surgery, 208, 313–320 (1988).
11. Chang D. K, Louis M. R, Gimenez A, Reece EM., The basics of Integra Dermal Regeneration Template and its expanding clinical applications. Semin Plast Surg. 33:185-189 (2019).
12. Opoku-Agyeman, J., Humenansky , K., Davis W. 3rd, Glat, P., Use of Integra for reconstruction after nevi resection: A systematic review and pooled analysis of reported cases, Surg Res Pract. 9: 9483627 (2019).
13. Gottlieb, M. E., Furman, J., Successful management and surgical closure of chronic and pathological wounds using Integra®. Journal of Burns and Surgical Wound Care, 3, 4 (2004)
14. Prezzavento G.E., Calvi R.N.J., Rodriguez J.A, Taupin P. Integra Dermal Regeneration Template in reconstructive surgery for cutaneous tumors. J Wound Care. 31:612-619 (2022).
15. Roels N., Van den Hof B., Integra® Dermal Regeneration Template as an alternative technique to treat degloving injury of fingers. Acta Chir Belg. 28:1-3 (2022).
16. Mogedas-Vegara A, Agut-Busquet E, Yébenes Marsal M, Luelmo Aguilar J, Escuder de la Torre Ò. Integra as Firstline Treatment for Scalp Reconstruction in Elderly Patients. J Oral Maxillofac Surg. 79:2593-2602 (2021).
17. Hulsen J, Diederich R, Neumeister MW, Bueno RA Jr. Hand (N Y). Integra® dermal regenerative template application on exposed tendon. 9:539-42 (2014).
18. Piejko M., Radziun K., Bobis-Wozowicz S., Waligórska A., Zimoląg E., Nessler M., Chrapusta A., Madeja Z., Drukała J. Adipose-derived stromal cells seeded on Integra® Dermal Regeneration Template improve post-burn wound reconstruction. Bioengineering (Basel). 7:67(2020).
19. Parshotam K. G., Barker, A., Ahmed, S. et al. Urinary bladder auto augmentation using INTEGRA® and SURGISIS®: an experimental model. Pediatr Surg Int 26, 275–280 (2010).
2. Yannas I.V., Tzeranis D.S., Mammals fail to regenerate organs when wound contraction drives scar formation. npj Regen Med 6, 39 (2021)
3. Hinz B, McCulloch C. A, Coelho N. M., Mechanical regulation of myofibroblast phenoconversion and collagen contraction. Exp Cell Res 379, 119-128 (2019).
4. Birk D. E., Trelstad R. L., “Fibroblasts compartmentalize the extracellular space to regulate and facilitate collagen fibril, bundle, and macro-aggregate formation”. In A. H. Reddi, Ed., Extracellular matrix: Structure and function. (Alan R. Liss. 1985).
5. Ferdman, A. G., Yannas I.V., Scattering of light from histologic sections: A new method for the analysis of connective tissue. J Invest Dermatol, 100, 710–716 (1993).
6. Yannas I. V., Lee, E., Orgill D. P., Skrabut E.M., Murphy G.F., Synthesis and characterization of a model extracellular matrix which induces partial regenera¬tion of adult mammalian skin. Proc Nat. Acad Sci USA 86, 933–937 (1989).
7. Tzeranis, D. S., Soller, E. C., Buydash, M. C., So, P. T., Yannas I. V., In situ quantification of surface chemistry in porous collagen biomaterials. Ann Biomed Eng 44, 803–815 (2016).
8. Burke, J. F., Yannas, I. V., Quinby, W. C., Jr., Bondoc, C. C., & Jung, W. K., Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Annals of Surgery, 194, 413–428 (1981).
9. Burke, J. F., Bondoc, C. C., & Quinby, W. C., Primary burn excision and immediate grafting: A method of shortening illness. The Journal of Trauma, 14, 389–395 (1974).
10. Heimbach, D., Luterman, A., Burke, J., Cram, A., Herndon, D., Hunt, J., Jordan, M., McManus, W., Solem, L., Warden, G., et al., Artificial dermis for major burns. Annals of Surgery, 208, 313–320 (1988).
11. Chang D. K, Louis M. R, Gimenez A, Reece EM., The basics of Integra Dermal Regeneration Template and its expanding clinical applications. Semin Plast Surg. 33:185-189 (2019).
12. Opoku-Agyeman, J., Humenansky , K., Davis W. 3rd, Glat, P., Use of Integra for reconstruction after nevi resection: A systematic review and pooled analysis of reported cases, Surg Res Pract. 9: 9483627 (2019).
13. Gottlieb, M. E., Furman, J., Successful management and surgical closure of chronic and pathological wounds using Integra®. Journal of Burns and Surgical Wound Care, 3, 4 (2004)
14. Prezzavento G.E., Calvi R.N.J., Rodriguez J.A, Taupin P. Integra Dermal Regeneration Template in reconstructive surgery for cutaneous tumors. J Wound Care. 31:612-619 (2022).
15. Roels N., Van den Hof B., Integra® Dermal Regeneration Template as an alternative technique to treat degloving injury of fingers. Acta Chir Belg. 28:1-3 (2022).
16. Mogedas-Vegara A, Agut-Busquet E, Yébenes Marsal M, Luelmo Aguilar J, Escuder de la Torre Ò. Integra as Firstline Treatment for Scalp Reconstruction in Elderly Patients. J Oral Maxillofac Surg. 79:2593-2602 (2021).
17. Hulsen J, Diederich R, Neumeister MW, Bueno RA Jr. Hand (N Y). Integra® dermal regenerative template application on exposed tendon. 9:539-42 (2014).
18. Piejko M., Radziun K., Bobis-Wozowicz S., Waligórska A., Zimoląg E., Nessler M., Chrapusta A., Madeja Z., Drukała J. Adipose-derived stromal cells seeded on Integra® Dermal Regeneration Template improve post-burn wound reconstruction. Bioengineering (Basel). 7:67(2020).
19. Parshotam K. G., Barker, A., Ahmed, S. et al. Urinary bladder auto augmentation using INTEGRA® and SURGISIS®: an experimental model. Pediatr Surg Int 26, 275–280 (2010).