Precancerous squamous intraepithelial lesions by human papillomavirus infection and p53 R72P polymorphism in Mexican women
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The aim was to determine the association between R72P polymorphism of p53 gene and the risk of developing squamous intraepithelial cervical lesions in HPV-16 and /or 18 infected women. Two groups of women were included in this study: 74 patients HPV-16 and /or 18 positive with a cytological and colposcopy diagnosis of squamous intraepithelial lesion and a group of unrelated 130 healthy blood-donors. The viral genotype, allele and genotype of the polymorphism frequencies were determined by PCR approached. The results were analyzed with the statistical programs DeFinetti and STAT intercooled v11.1. Patients with high-grade squamous intrahepitelial lesions (HG-SIL) were infected mainly by HPV-16 (60.72%) compared to low-grade lesions (LG-SIL) (39.28%) (OR 3.14; p= 0.037), with HPV-18 genotype 68.96% of LG-SIL and 31.04% were HG-SIL (OR=0.24, p=0.006). HG-SIL were more common in patients carrying both viral genotypes (70.59% vs 29.41%) (OR 2.8, p = 0.008). A statistically significant association was observed between the genotype R/R and HG-SIL (OR=11.25, IC 3.8-33.29, p= 0.000) compared to those with LG-SIL. The P/R genotype was significantly more frequent in patients LG-SIL, compared to HG-SIL (OR=0.27, p=0.00). In conclusion patients with the R/R genotype showed more susceptibility to HPV-16 infection and they have almost 12 times more risk probability of HG-SIL compared to women having the heterozygous genotype and HPV-18.
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Chan, K.W., Lam, K.Y., Chan, A.C.L., Lau, P., and Srivastava, G. (1994). Prevalence of human papillomavirus types 16 and 18 in penile carcinoma: a study of 41 cases using PCR. Journal of Clinical Pathology, 47, 823-26.
El khair, M.M., Ennaji, M.M., El kebbaj, R., Mhand, R.A., Attaleb, M., and El Mzibri, M. (2010). p53 codon 72 polymorphism and risk of cervical carcinoma in Moroccan women. Medical Oncology, 27, 861-66
El tahir, H.A., Adam, A.A., Yahia, Z.A., Ali, N.F., Mursi, D.M., Higazi, A.M., … Ibrahim, M.E. (2012). p53 codon 72 arginine/proline polymorphism and cancer in Sudan. Molecular Biology Reports, 39(12), 10833-10836.
Gustincich, S., Manfioletti, G., Del Sal, G., Schneider. C., and Carninci, P. (1991). A fast method for high-quality genomic DNA extraction from whole human blood. Biotechniques, 11(3), 298-300.
Habbous, S., Pang, V., Eng, L., Xu, W., Kurtz, G., Liu, F.F., … Liu, G. (2012). p53 Arg72Pro polymorphism, HPV status and Initiation, progression, and development of cervical cancer: A systematic review and meta-analysis. Clinical Cancer Research, 18(23), 6407-6415.
Klug, S.J., Ressing, M., Koenig, J., Abba, M.C., Agorastos, T., Brenna, … Blettner, M. (2009). TP53 codon 72 polymorphism and cervical cancer: a pooled analysis of individual data from 49 studies. Lancet Oncol, 10(8), 772-784.
Lopez-Saavedra, A. and Lizano-Soberon, M. (2006). Cancer cervicouterino y el virus del papiloma humano: la historia que no termina. Cancerologia, 1, 31-55.
Piña-Sanchez, P., Hernandez-Hernandez, D.M., Taja-Chayb, L., Cerda-Flores, R.M., González-Herrera, A.L., … Salcedo, M. (2011). Polymorphism in exon 4 of TP53 gene associated to HPV 16 and 18 in Mexican women with cervical cancer. Medical Oncology, 28(4), 1507-1513.
Proestling, K., Hebar, A., Pruckner, N., Marton, E., Vinatzer, U., and Schreiber, M. (2012). The Pro allele of the p53 codon 72 polymorphism is associated with decreased intratumoral expression of BAX and p21, and increased breast cancer risk. PLoS One, 7(10). doi:10.1371/journal.pone.0047325.
Richard, C., Lanner, C., Naryzhny, S.N., Sherman, L., Lee, H., Lambert, P.F., and Zehbe, I. (2010). The immortalizing and transforming ability of two common human papillomavirus 16 E6 variants with different prevalences in cervical cancer. Oncogene, 29(23), 3435-3445.
Salazar, E.L., Mercado, E., and Calzada, L. (2005). Human papillomavirus HPV-16 DNA an epitheliotropic virus that induces hyperproliferation in squamous pennile tissue. Archives of Andrology, 51, 327-334.
Sifuentes-Alvarez, A., and Reyes-Romero, M. (2003). Risk factors for cervico-uterine cancer associated to HPV: p53 codon 72 polymorphism in women attending hospital care. Ginecologia y Obstetricia de Mexico, 71, 12-15.
Smith, J.S., Lindsay, L., Hoots, B., Keys, J., Franceschi, S., Winer, R., and Clifford, G.M. (2007). Human papillomavirus type distribution in invasive cervical cancer and high-grade cervical lesions: A meta-analysis update. International Journal of Cancer, 121(3), 621–632.
Sousa, H., Santos, A.M., Pinto, D., and Medeiros, R. (2007). Is the p53 codon 72 polymorphism a key biomarker for cervical cancer development? A meta-analysis review within European populations. International Journal of Molecular Medicine, 20, 731-741.
Storey, A., Thomas, M., Kalita, A., Harwood, C., Gardiol, D.,
Mantovani, F., … Banks, L. (1998). Role of a p53 polymorphism in the development of human papillomavirus- associated cancer. Nature, 393, 229-34.
Suarez-Rincon, A.E., Moran-Moguel, M.C., Montoya-Fuentes, H., Gallegos-Arreola, M.P., and Sánchez-Corona, J. (2002). Polymorphism in codon 72 of the p53 gene and cervico-uterine cancer risk in Mexico. Ginecología y Obstetricia de Mexico, 70, 344-8.
Zur Hausen H. (2009). Papillomaviruses in the causation of human cancers a brief historical account. Virology, 384(2), 260-265.