The Upcoming Drift of Organ-Chip in Pharmacological Trial Investigate
Main Article Content
Abstract
The urgent need for a novel way to replicate human drug reactions in preclinical research has motivated the development of organ-on-a-chip (OoC) systems. These difficulties are not recognized during preclinical trials, owing to ineffective screening technologies that mimic the complexities of human tissues and provide quick, accurate screening readouts. Microfluidics and microfabrication are powerful methods for creating numerous systems with high spatiotemporal precision to simulate in vivo microenvironments for drug delivery, discovery, development, and screening. This method might be used to investigate cell responses to pharmacological and mechanical stimuli in a more physiologically effective way. In this paper, we examine current achievements in OoC with an emphasis on biomimicry, functionality, and characteristics, as well as multi-organ platforms that attempt to replicate the essential aspects of integrated human physiology. Finally, we explore future prospects and limits that must be addressed in order to get OoC systems closer to clinical translation.
Keywords:
organ chip, future prospective, multi-organ chip, limitation
Article Details
How to Cite
UBAIDULLA, Dr. U. et al.
The Upcoming Drift of Organ-Chip in Pharmacological Trial Investigate.
Medical Research Archives, [S.l.], v. 11, n. 11, nov. 2023.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/4741>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.18103/mra.v11i11.4741.
Section
Research Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
1. Amin Valiei, Javad Aminian-Dehkordi, Mohammad R. K. Mofrad. Gut-on-a-chip models for dissecting the gut microbiology and physiology. APL Bioengineering .2023; 7 (1): 011502.
2. Linda Sønstevold, Maciej Czerkies, Enrique Escobedo-Cousin, Slawomir Blonski, Elizaveta Vereshchagina. Application of Polymethylpentene, an Oxygen Permeable Thermoplastic, for Long-Term on-a-Chip Cell Culture and Organ-on-a-Chip Devices. Micromachines. 2023;14 (3): 532.
3. Uyen M. N. Cao, Yuli Zhang, Julie Chen, Darren Sayson, Sangeeth Pillai, Simon D. Tran. Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication. International Journal of Molecular Science.s 2023; 24 (4):3232.
4. Mengde Zhang, Chao Zhang, Zhao Li, Xiaobing Fu, Sha Huang. Advances in 3D skin bioprinting for wound healing and disease modeling. Regenerative Biomaterials .2023; 10
5. Nianfang Hu, Kerun Cheng, Shuhan Zhang, Shan Liu, Lijun Wang, Xiaoxin Du, Yong Li, Chenzhong Li. Advancements in microfluidics for skin cosmetic screening. The Analyst. 2023; L342
6. Juan F. Hernández-Rodríguez, Miguel Ángel López, Daniel Rojas, Alberto Escarpa. Digital manufacturing for accelerating organ-on-a-chip dissemination and electrochemical biosensing integration. Lab on a Chip. 2022; 22 (24): 4805-4821.
7. Sumira Malik, Archna Dhasmana, Subham Preetam, Yogendra Kumar Mishra, Vishal Chaudhary, Sweta Parmita Bera, Anuj Ranjan, Jutishna Bora, Ajeet Kaushik, Tatiana Minkina, Hanuman Singh Jatav, Rupesh Kumar Singh, Vishnu D. Rajput. Exploring Microbial-Based Green Nanobiotechnology for Wastewater Remediation: A Sustainable Strategy. Nanomaterials. 2022;12 (23): 4187.
8. anima Bhattacharya, Subham Preetam, Basab Ghosh, Tulika Chakrabarti, Prasun Chakrabarti, Shailesh Kumar Samal, Nanasaheb Thorat. Advancement in Biopolymer Assisted Cancer Theranostics. ACS Applied Bio Materials. 2023. Article ASAP.
9. Do-Hyun Oh, Inseong Choi, Young-Hyun Ryu, Guk-Young Ahn, Tae-Kyung Ryu, Sung-Wook Choi. Solvent-Resistant Perfluoropolyether Microfluidic Devices with Microfibrous Channels for the Production of Poly(ε-caprolactone) Microspheres Containing Dexamethasone. ACS Applied Polymer Materials. 2023; 5 (3): 2062-2069.
10. Rahul Bhattacharjee, Arvind Negi, Basudha Bhattacharya, Tanima Dey, Priya Mitra, Subham Preetam, Lamha Kumar, Sulagna Kar, Sabya Sachi Das, Danish Iqbal, Mehnaz Kamal, Fayez Alghofaili, Sumira Malik, Abhijit Dey, Saurabh Kumar Jha, Shreesh Ojha, Ana Cláudia Paiva-Santos, Kavindra Kumar Kesari, Niraj Kumar Jha. Nanotheranostics to Target Antibiotic-resistant Bacteria: Strategies and Applications. OpenNano. 2023; 23: 100138.
11. urachate Kalasin, Pantawan Sangnuang, Werasak Surareungchai. Satellite-Based Sensor for Environmental Heat-Stress Sweat Creatinine Monitoring: The Remote Artificial Intelligence-Assisted Epidermal Wearable Sensing for Health Evaluation. ACS Biomaterials Science & Engineering. 2021;7 (1): 322-334.
12. Emma L. Vanderlaan, Joshua Sexton, Carmella Evans-Molina, Adrian Buganza Tepole, Sherry L. Voytik-Harbin. Islet-on-chip: promotion of islet health and function via encapsulation within a polymerizable fibrillar collagen scaffold. Lab on a Chip .2023; 23 (20): 4466-4482.
https://doi.org/10.1039/D3LC00371J
13. Fida Hussain Memon, Kang Inseok, Faheem Ahmed, Wajid Ali, Afaque Manzoor Soomro, Saleem Raza Memon, Kashif Hussain Memon, Kyung Hyun Choi. A Comprehensive Review of Biosensor Integration in Microphysiological Systems for Online Monitoring: Current Challenges and Future Advancements. ChemBioEng Reviews. 2023; 10 (5): 817-828.
14. Antonia van Rijt, Evan Stefanek, Karolina Valente. Preclinical Testing Techniques: Paving the Way for New Oncology Screening Approaches. Cancers .2023; 15 (18): 4466.
15. Fuki Yokoi, Sayaka Deguchi, Kazuo Takayama. Organ-on-a-chip models for elucidating the cellular biology of infectious diseases. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2023; 1870 (6): 119504.
16. Ziqiu Tong, Lars Esser, Peter Galettis, David Rudd, Christopher D. Easton, Azadeh Nilghaz, Bo Peng, Douer Zhu, Helmut Thissen, Jennifer H. Martin, Nicolas H. Voelcker. Fluoropolymer Functionalization of Organ-on-Chip Platform Increases Detection Sensitivity for Cannabinoids. Biosensor.s 2023; 13 (8): 779.
17. Yunyun Wu, Claire Liu, Mia Lapiere, Joanna L. Ciatti, Da Som Yang, Jaime Berkovich, Jeffrey B. Model, Anthony Banks, Roozbeh Ghaffari, Jan‐Kai Chang, Ralph G. Nuzzo, John A. Rogers. Thermoplastic Elastomers for Wireless, Skin‐Interfaced Electronic, and Microfluidic Devices. Advanced Materials Technologies. 2023; 5
18. Sean Hockney, Jessica Parker, Jasmin E. Turner, Xanthea Todd, Stephen Todryk, Roben Ger Gieling, Gerrit Hilgen, Davina Camargo Madeira Simoes, Deepali Pal. Next generation organoid engineering to replace animals in cancer drug testing. Biochemical Pharmacology. 2023; 213: 115586.
19. Zhong Alan Li, Shilpa Sant, Sung Kwon Cho, Stuart B. Goodman, Bruce A. Bunnell, Rocky S. Tuan, Michael S. Gold, Hang Lin. Synovial joint-on-a-chip for modeling arthritis: progress, pitfalls, and potential. Trends in Biotechnology .2023; 41 (4):511-527.
20. Victoria E.J.M. Palasantzas, Isabel Tamargo-Rubio, Kieu Le, Jelle Slager, Cisca Wijmenga, Iris H. Jonkers, Vinod Kumar, Jingyuan Fu, Sebo Withoff. iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies. Trends in Genetics. 2023; 39 (4):268-284.
21. Daniel J. Teixeira Carvalho, Lorenzo Moroni, Stefan Giselbrecht. Clamping strategies for organ-on-a-chip devices. Nature Reviews Materials. 2023;8 (3): 147-164.
22. Yashi Sisodia, Komal Shah, Adil Ali Sayyed, Meenakshi Jain, Syed Ansar Ali, Piyush Gondaliya, Kiran Kalia, Rakesh Kumar Tekade. Lung-on-chip microdevices to foster pulmonary drug discovery. Biomaterials Science. 2023;11 (3): 777-790.
23. Cristina Marisol Castillo Bautista, Jared Sterneckert. Progress and challenges in directing the differentiation of human iPSCs into spinal motor neurons. Frontiers in Cell and Developmental Biology. 2023;10
24. ngrid Anaya Morales, Christina-Marie Boghdady, Benjamin E. Campbell, Christopher Moraes. Integrating mechanical sensor readouts into organ-on-a-chip platforms. Frontiers in Bioengineering and Biotechnology .2022;10
25. Juan F. Hernández-Rodríguez, Miguel Ángel López, Daniel Rojas, Alberto Escarpa. Digital manufacturing for accelerating organ-on-a-chip dissemination and electrochemical biosensing integration. Lab on a Chip .2022;22 (24): 4805-4821. https://doi.org/10.1039/D2LC00499B
26. Chuan Liu, Scott B. Campbell, Jianzhao Li, Dawn Bannerman, Simon Pascual‐Gil, Jennifer Kieda, Qinghua Wu, Peter R. Herman, Milica Radisic. High Throughput Omnidirectional Printing of Tubular Microstructures from Elastomeric Polymers. Advanced Healthcare Materials. 2022; 11 (23)
27. Mahsa Akbari Kenari, Erfan Rezvani Ghomi, Amirreza Akbari Kenari, Seyed Mohammad Saeed Arabi, Javad Deylami, Seeram Ramakrishna. Biomedical applications of microfluidic devices: Achievements and challenges. Polymers for Advanced Technologies .2022; 33 (12): 3920-3934.
28. Alessio Bucciarelli, Xenia Paolelli, Eleonora De Vitis, Nora Selicato, Francesca Gervaso, Giuseppe Gigli, Lorenzo Moroni, Alessandro Polini. VAT photopolymerization 3D printing optimization of high aspect ratio structures for additive manufacturing of chips towards biomedical applications. Additive Manufacturing. 2022; 60: 103200.
29. V. Frantellizzi, V. Verrina, C. Raso, M. Pontico, F. Petronella, V. Bertana, A. Ballesio, S.L. Marasso, S. Miglietta, P. Rosa, S. Scibetta, V. Petrozza, M.S. De Feo, G. De Vincentis, A. Calogero, R. Pani, G. Perotto, L. De Sio. 99mTc-labeled keratin gold-nanoparticles in a nephron-like microfluidic chip for photo-thermal therapy applications. Materials Today Advances. 2022; 16: 100286.
30. Elsbeth G. B. M. Bossink, Anke R. Vollertsen, Joshua T. Loessberg-Zahl, Andries D. van der Meer, Loes I. Segerink, Mathieu Odijk. Systematic characterization of cleanroom-free fabricated macrovalves, demonstrating pumps and mixers for automated fluid handling tuned for organ-on-chip applications. Microsystems & Nanoengineering .2022; 8 (1)
31. Zhenxu Yang, Xiaochen Liu, Elise M. Cribbin, Alice M. Kim, Jiao Jiao Li, Ken-Tye Yong. Liver-on-a-chip: Considerations, advances, and beyond. Biomicrofluidic.s 2022;16 (6)
32. Mohammad Hossein Mohammadi, Sargol Okhovatian, Houman Savoji, Scott B. Campbell, Benjamin Fook Lun Lai, Jun Wu, Simon Pascual‐Gil, Dawn Bannerman, Naimeh Rafatian, Ren‐Ke Li, Milica Radisic. Toward Hierarchical Assembly of Aligned Cell Sheets into a Conical Cardiac Ventricle Using Microfabricated Elastomers. Advanced Biology. 2022; 6 (11)
33. Mehran Mansouri, Adeel Ahmed, S. Danial Ahmad, Molly C. McCloskey, Indranil M. Joshi, Thomas R. Gaborski, Richard E. Waugh, James L. McGrath, Steven W. Day, Vinay V. Abhyankar. The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow. Advanced Healthcare Materials. 2022; 11 (21)
34. Mengyang Wang, Masahiro Tsuda, Sayaka Deguchi, Yuriko Higuchi, Kanako So, Yu-suke Torisawa, Kazuo Takayama, Fumiyoshi Yamashita. Application of perfluoropolyether elastomers in microfluidic drug metabolism assays. International Journal of Pharmaceutics. 2022; 627: 122253.
35. Lincao Jiang, Qiwei Li, Weicheng Liang, Xuan Du, Yi Yang, Zilin Zhang, Lili Xu, Jing Zhang, Jian Li, Zaozao Chen, Zhongze Gu. Organ-On-A-Chip Database Revealed—Achieving the Human Avatar in Silicon. Bioengineering .2022; 9 (11): 685. https://doi.org/10.3390/bioengineering9110685
36. Ruojun Mu, Nitong Bu, Jie Pang, Lin Wang, Yue Zhang. Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications. Foods. 2022; 11 (22):3727. https://doi.org/10.3390/foods11223727
37. Leire Etxeberria, Taha Messelmani, Jon Haitz Badiola, Andreu Llobera, Luis Fernandez, José Luis Vilas-Vilela, Eric Leclerc, Cécile Legallais, Rachid Jellali, Ane Miren Zaldua. Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors. Polymers. 2022; 14 (21): 4478.
38. Ali Doryab, Mehmet Berat Taskin, Philipp Stahlhut, Jürgen Groll, Otmar Schmid. Real‐Time Measurement of Cell Mechanics as a Clinically Relevant Readout of an In Vitro Lung Fibrosis Model Established on a Bioinspired Basement Membrane. Advanced Materials. 2022; 34 (41)
39. A M van Genderen, M G Valverde, P E Capendale, M V Kersten, E Sendino Garví, C C L Schuurmans, M Ruelas, J T Soeiro, G Tang, M J Janssen, J Jansen, S M Mihăilă, T Vermonden, Y S Zhang, R Masereeuw. Co-axial printing of convoluted proximal tubule for kidney disease modeling. Biofabrication.2022; 14 (4): 044102.
40. Karen Melissa Lerin-Morales, Luis F. Olguín, Eva Mateo-Martí, María Colín-García. Prebiotic Chemistry Experiments Using Microfluidic Devices. Life. 2022; 12 (10):1665.
2. Linda Sønstevold, Maciej Czerkies, Enrique Escobedo-Cousin, Slawomir Blonski, Elizaveta Vereshchagina. Application of Polymethylpentene, an Oxygen Permeable Thermoplastic, for Long-Term on-a-Chip Cell Culture and Organ-on-a-Chip Devices. Micromachines. 2023;14 (3): 532.
3. Uyen M. N. Cao, Yuli Zhang, Julie Chen, Darren Sayson, Sangeeth Pillai, Simon D. Tran. Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication. International Journal of Molecular Science.s 2023; 24 (4):3232.
4. Mengde Zhang, Chao Zhang, Zhao Li, Xiaobing Fu, Sha Huang. Advances in 3D skin bioprinting for wound healing and disease modeling. Regenerative Biomaterials .2023; 10
5. Nianfang Hu, Kerun Cheng, Shuhan Zhang, Shan Liu, Lijun Wang, Xiaoxin Du, Yong Li, Chenzhong Li. Advancements in microfluidics for skin cosmetic screening. The Analyst. 2023; L342
6. Juan F. Hernández-Rodríguez, Miguel Ángel López, Daniel Rojas, Alberto Escarpa. Digital manufacturing for accelerating organ-on-a-chip dissemination and electrochemical biosensing integration. Lab on a Chip. 2022; 22 (24): 4805-4821.
7. Sumira Malik, Archna Dhasmana, Subham Preetam, Yogendra Kumar Mishra, Vishal Chaudhary, Sweta Parmita Bera, Anuj Ranjan, Jutishna Bora, Ajeet Kaushik, Tatiana Minkina, Hanuman Singh Jatav, Rupesh Kumar Singh, Vishnu D. Rajput. Exploring Microbial-Based Green Nanobiotechnology for Wastewater Remediation: A Sustainable Strategy. Nanomaterials. 2022;12 (23): 4187.
8. anima Bhattacharya, Subham Preetam, Basab Ghosh, Tulika Chakrabarti, Prasun Chakrabarti, Shailesh Kumar Samal, Nanasaheb Thorat. Advancement in Biopolymer Assisted Cancer Theranostics. ACS Applied Bio Materials. 2023. Article ASAP.
9. Do-Hyun Oh, Inseong Choi, Young-Hyun Ryu, Guk-Young Ahn, Tae-Kyung Ryu, Sung-Wook Choi. Solvent-Resistant Perfluoropolyether Microfluidic Devices with Microfibrous Channels for the Production of Poly(ε-caprolactone) Microspheres Containing Dexamethasone. ACS Applied Polymer Materials. 2023; 5 (3): 2062-2069.
10. Rahul Bhattacharjee, Arvind Negi, Basudha Bhattacharya, Tanima Dey, Priya Mitra, Subham Preetam, Lamha Kumar, Sulagna Kar, Sabya Sachi Das, Danish Iqbal, Mehnaz Kamal, Fayez Alghofaili, Sumira Malik, Abhijit Dey, Saurabh Kumar Jha, Shreesh Ojha, Ana Cláudia Paiva-Santos, Kavindra Kumar Kesari, Niraj Kumar Jha. Nanotheranostics to Target Antibiotic-resistant Bacteria: Strategies and Applications. OpenNano. 2023; 23: 100138.
11. urachate Kalasin, Pantawan Sangnuang, Werasak Surareungchai. Satellite-Based Sensor for Environmental Heat-Stress Sweat Creatinine Monitoring: The Remote Artificial Intelligence-Assisted Epidermal Wearable Sensing for Health Evaluation. ACS Biomaterials Science & Engineering. 2021;7 (1): 322-334.
12. Emma L. Vanderlaan, Joshua Sexton, Carmella Evans-Molina, Adrian Buganza Tepole, Sherry L. Voytik-Harbin. Islet-on-chip: promotion of islet health and function via encapsulation within a polymerizable fibrillar collagen scaffold. Lab on a Chip .2023; 23 (20): 4466-4482.
https://doi.org/10.1039/D3LC00371J
13. Fida Hussain Memon, Kang Inseok, Faheem Ahmed, Wajid Ali, Afaque Manzoor Soomro, Saleem Raza Memon, Kashif Hussain Memon, Kyung Hyun Choi. A Comprehensive Review of Biosensor Integration in Microphysiological Systems for Online Monitoring: Current Challenges and Future Advancements. ChemBioEng Reviews. 2023; 10 (5): 817-828.
14. Antonia van Rijt, Evan Stefanek, Karolina Valente. Preclinical Testing Techniques: Paving the Way for New Oncology Screening Approaches. Cancers .2023; 15 (18): 4466.
15. Fuki Yokoi, Sayaka Deguchi, Kazuo Takayama. Organ-on-a-chip models for elucidating the cellular biology of infectious diseases. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2023; 1870 (6): 119504.
16. Ziqiu Tong, Lars Esser, Peter Galettis, David Rudd, Christopher D. Easton, Azadeh Nilghaz, Bo Peng, Douer Zhu, Helmut Thissen, Jennifer H. Martin, Nicolas H. Voelcker. Fluoropolymer Functionalization of Organ-on-Chip Platform Increases Detection Sensitivity for Cannabinoids. Biosensor.s 2023; 13 (8): 779.
17. Yunyun Wu, Claire Liu, Mia Lapiere, Joanna L. Ciatti, Da Som Yang, Jaime Berkovich, Jeffrey B. Model, Anthony Banks, Roozbeh Ghaffari, Jan‐Kai Chang, Ralph G. Nuzzo, John A. Rogers. Thermoplastic Elastomers for Wireless, Skin‐Interfaced Electronic, and Microfluidic Devices. Advanced Materials Technologies. 2023; 5
18. Sean Hockney, Jessica Parker, Jasmin E. Turner, Xanthea Todd, Stephen Todryk, Roben Ger Gieling, Gerrit Hilgen, Davina Camargo Madeira Simoes, Deepali Pal. Next generation organoid engineering to replace animals in cancer drug testing. Biochemical Pharmacology. 2023; 213: 115586.
19. Zhong Alan Li, Shilpa Sant, Sung Kwon Cho, Stuart B. Goodman, Bruce A. Bunnell, Rocky S. Tuan, Michael S. Gold, Hang Lin. Synovial joint-on-a-chip for modeling arthritis: progress, pitfalls, and potential. Trends in Biotechnology .2023; 41 (4):511-527.
20. Victoria E.J.M. Palasantzas, Isabel Tamargo-Rubio, Kieu Le, Jelle Slager, Cisca Wijmenga, Iris H. Jonkers, Vinod Kumar, Jingyuan Fu, Sebo Withoff. iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies. Trends in Genetics. 2023; 39 (4):268-284.
21. Daniel J. Teixeira Carvalho, Lorenzo Moroni, Stefan Giselbrecht. Clamping strategies for organ-on-a-chip devices. Nature Reviews Materials. 2023;8 (3): 147-164.
22. Yashi Sisodia, Komal Shah, Adil Ali Sayyed, Meenakshi Jain, Syed Ansar Ali, Piyush Gondaliya, Kiran Kalia, Rakesh Kumar Tekade. Lung-on-chip microdevices to foster pulmonary drug discovery. Biomaterials Science. 2023;11 (3): 777-790.
23. Cristina Marisol Castillo Bautista, Jared Sterneckert. Progress and challenges in directing the differentiation of human iPSCs into spinal motor neurons. Frontiers in Cell and Developmental Biology. 2023;10
24. ngrid Anaya Morales, Christina-Marie Boghdady, Benjamin E. Campbell, Christopher Moraes. Integrating mechanical sensor readouts into organ-on-a-chip platforms. Frontiers in Bioengineering and Biotechnology .2022;10
25. Juan F. Hernández-Rodríguez, Miguel Ángel López, Daniel Rojas, Alberto Escarpa. Digital manufacturing for accelerating organ-on-a-chip dissemination and electrochemical biosensing integration. Lab on a Chip .2022;22 (24): 4805-4821. https://doi.org/10.1039/D2LC00499B
26. Chuan Liu, Scott B. Campbell, Jianzhao Li, Dawn Bannerman, Simon Pascual‐Gil, Jennifer Kieda, Qinghua Wu, Peter R. Herman, Milica Radisic. High Throughput Omnidirectional Printing of Tubular Microstructures from Elastomeric Polymers. Advanced Healthcare Materials. 2022; 11 (23)
27. Mahsa Akbari Kenari, Erfan Rezvani Ghomi, Amirreza Akbari Kenari, Seyed Mohammad Saeed Arabi, Javad Deylami, Seeram Ramakrishna. Biomedical applications of microfluidic devices: Achievements and challenges. Polymers for Advanced Technologies .2022; 33 (12): 3920-3934.
28. Alessio Bucciarelli, Xenia Paolelli, Eleonora De Vitis, Nora Selicato, Francesca Gervaso, Giuseppe Gigli, Lorenzo Moroni, Alessandro Polini. VAT photopolymerization 3D printing optimization of high aspect ratio structures for additive manufacturing of chips towards biomedical applications. Additive Manufacturing. 2022; 60: 103200.
29. V. Frantellizzi, V. Verrina, C. Raso, M. Pontico, F. Petronella, V. Bertana, A. Ballesio, S.L. Marasso, S. Miglietta, P. Rosa, S. Scibetta, V. Petrozza, M.S. De Feo, G. De Vincentis, A. Calogero, R. Pani, G. Perotto, L. De Sio. 99mTc-labeled keratin gold-nanoparticles in a nephron-like microfluidic chip for photo-thermal therapy applications. Materials Today Advances. 2022; 16: 100286.
30. Elsbeth G. B. M. Bossink, Anke R. Vollertsen, Joshua T. Loessberg-Zahl, Andries D. van der Meer, Loes I. Segerink, Mathieu Odijk. Systematic characterization of cleanroom-free fabricated macrovalves, demonstrating pumps and mixers for automated fluid handling tuned for organ-on-chip applications. Microsystems & Nanoengineering .2022; 8 (1)
31. Zhenxu Yang, Xiaochen Liu, Elise M. Cribbin, Alice M. Kim, Jiao Jiao Li, Ken-Tye Yong. Liver-on-a-chip: Considerations, advances, and beyond. Biomicrofluidic.s 2022;16 (6)
32. Mohammad Hossein Mohammadi, Sargol Okhovatian, Houman Savoji, Scott B. Campbell, Benjamin Fook Lun Lai, Jun Wu, Simon Pascual‐Gil, Dawn Bannerman, Naimeh Rafatian, Ren‐Ke Li, Milica Radisic. Toward Hierarchical Assembly of Aligned Cell Sheets into a Conical Cardiac Ventricle Using Microfabricated Elastomers. Advanced Biology. 2022; 6 (11)
33. Mehran Mansouri, Adeel Ahmed, S. Danial Ahmad, Molly C. McCloskey, Indranil M. Joshi, Thomas R. Gaborski, Richard E. Waugh, James L. McGrath, Steven W. Day, Vinay V. Abhyankar. The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow. Advanced Healthcare Materials. 2022; 11 (21)
34. Mengyang Wang, Masahiro Tsuda, Sayaka Deguchi, Yuriko Higuchi, Kanako So, Yu-suke Torisawa, Kazuo Takayama, Fumiyoshi Yamashita. Application of perfluoropolyether elastomers in microfluidic drug metabolism assays. International Journal of Pharmaceutics. 2022; 627: 122253.
35. Lincao Jiang, Qiwei Li, Weicheng Liang, Xuan Du, Yi Yang, Zilin Zhang, Lili Xu, Jing Zhang, Jian Li, Zaozao Chen, Zhongze Gu. Organ-On-A-Chip Database Revealed—Achieving the Human Avatar in Silicon. Bioengineering .2022; 9 (11): 685. https://doi.org/10.3390/bioengineering9110685
36. Ruojun Mu, Nitong Bu, Jie Pang, Lin Wang, Yue Zhang. Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications. Foods. 2022; 11 (22):3727. https://doi.org/10.3390/foods11223727
37. Leire Etxeberria, Taha Messelmani, Jon Haitz Badiola, Andreu Llobera, Luis Fernandez, José Luis Vilas-Vilela, Eric Leclerc, Cécile Legallais, Rachid Jellali, Ane Miren Zaldua. Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors. Polymers. 2022; 14 (21): 4478.
38. Ali Doryab, Mehmet Berat Taskin, Philipp Stahlhut, Jürgen Groll, Otmar Schmid. Real‐Time Measurement of Cell Mechanics as a Clinically Relevant Readout of an In Vitro Lung Fibrosis Model Established on a Bioinspired Basement Membrane. Advanced Materials. 2022; 34 (41)
39. A M van Genderen, M G Valverde, P E Capendale, M V Kersten, E Sendino Garví, C C L Schuurmans, M Ruelas, J T Soeiro, G Tang, M J Janssen, J Jansen, S M Mihăilă, T Vermonden, Y S Zhang, R Masereeuw. Co-axial printing of convoluted proximal tubule for kidney disease modeling. Biofabrication.2022; 14 (4): 044102.
40. Karen Melissa Lerin-Morales, Luis F. Olguín, Eva Mateo-Martí, María Colín-García. Prebiotic Chemistry Experiments Using Microfluidic Devices. Life. 2022; 12 (10):1665.