Alterations between Effective and Ineffective Multipotent Mesenchymal Stromal Cells Used for Acute Graft Versus Host Disease Prophylaxis
Main Article Content
Nina Drize
http://orcid.org/0000-0002-7150-0403
Nataliya Petinati
http://orcid.org/0000-0001-6591-3183
Irina Shupounova
http://orcid.org/0000-0003-1189-0283
Natalia Sats
http://orcid.org/0000-0002-1559-9381
Alexey Bigildeev
http://orcid.org/0000-0003-0215-9085
Larisa Kuzmina
http://orcid.org/0000-0001-6201-6276
Elena Parovichnikova
http://orcid.org/0000-0002-1825-0097
Valeriy Savchenko
http://orcid.org/0000-0001-8188-5557
http://orcid.org/0000-0002-7150-0403
Nataliya Petinati
http://orcid.org/0000-0001-6591-3183
Irina Shupounova
http://orcid.org/0000-0003-1189-0283
Natalia Sats
http://orcid.org/0000-0002-1559-9381
Alexey Bigildeev
http://orcid.org/0000-0003-0215-9085
Larisa Kuzmina
http://orcid.org/0000-0001-6201-6276
Elena Parovichnikova
http://orcid.org/0000-0002-1825-0097
Valeriy Savchenko
http://orcid.org/0000-0001-8188-5557
Abstract
Multipotent mesenchymal stromal cells (MSCs) are applied for prophylaxis of acute graft versus host disease (aGvHD) after allogeneic hematopoietic cell transplantation (allo-HCT). Not all samples of MSC used in National Research Center for Hematology were efficient for aGvHD prevention. The suitabilityof MSCs for aGvHD prophylaxis was studied. MSCs derived from the bone marrow of HCT donor were injected intravenously precisely at the moment of blood cell reconstitution. MSCs were cultivated for 3 passages. The characteristics of donor bone marrow samples including colony forming unit fibroblast (CFU-F) concentration, growth parameters of MSCs and the relative expression levels (REL) of different genes in them were analyzed. MSCs infusion induced a decrease in aGvHD development in patients with related and unrelated donors compared with the standard prophylaxis group. aGvHD prophylaxis with MSCs was ineffective in 13.5% of cases. In these MSC samples, a significant decrease in total cell production and the REL of CFH, FGFR1, PDGFRa and ICAM1 were observed. This study showed that MSCs injection resulted in a significant 2-fold decrease in aGvHD development compared with patients in the standard prophylaxis group. The effective MSC samples are characterized by higher total cell production and REL of CFH, FGFR1, PDGFRa and ICAM1 than ineffective.
Article Details
How to Cite
DRIZE, Nina et al.
Alterations between Effective and Ineffective Multipotent Mesenchymal Stromal Cells Used for Acute Graft Versus Host Disease Prophylaxis.
Medical Research Archives, [S.l.], v. 4, n. 1, june 2016.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/501>. Date accessed: 23 nov. 2024.
Keywords
aGvHD prophylaxis, MSC, CFU-F, gene expression
Section
Research Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
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Meisel, R., Zibert, A., Laryea, M., Göbel, U., Däubener, W., & Dilloo, D. (2004). Human bone marrow stromal cells inhibit allogeneic T-cell responses by indoleamine 2,3-dioxygenase-mediated tryptophan degradation. Blood, 103(12), 4619–21. http://doi.org/10.1182/blood-2003-11-3909
Menssen, A., Häupl, T., Sittinger, M., Delorme, B., Charbord, P., & Ringe, J. (2011). Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development. BMC Genomics, 12(1), 461. http://doi.org/10.1186/1471-2164-12-461
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