Histological Visualization of Glyceraldehyde-Derived Glycation with Glucose using Ultrasound Microscopy

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Published Feb 27, 2024
DOI: https://doi.org/10.18103/mra.v12i2.5035

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Main Article Content

Katsutoshi Miura
Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, Japan
Toshihide Iwashita
Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, Japan

Abstract

Background: Reducing sugars and reactive aldehydes, such as glyceraldehyde (GA), non-enzymatically react with proteins to form advanced glycation end-products (AGEs). GA is produced in the glycolysis pathway, and GA-derived AGEs play an essential role in the pathogenesis of angiopathy associated with hyperglycemia in patients with diabetes.


Aims: Several studies have reported on chemical alterations in glycation. However, histological confirmation of these biochemical changes has been relatively rare. This study aimed to visualize glyceraldehyde-induced glycation and evaluate the severity of glycation using attenuation of sound (AOS) values. Given that glycation promotes cross-links between proteins and sugars, the energy loss of sound that passes through them increases. We hypothesized that AOS alteration would reflect the glycation state of the tissues and cells.


Methods: Fresh frozen sections or fresh cells were briefly fixed in ethanol and soaked in GA with different glucose concentrations. Thereafter, AOS images were obtained via scanning acoustic microscopy over time, and tissue and cellular glycation induced by GA with glucose was evaluated using AOS values.


Results: AOS images were able to visualize GA-induced glycation over time. Compared to GA alone, glucose supplements concentration-dependently accelerated glycation. The arterial smooth muscle, collagen, and intima were apt to accept glycation, whereas the mucosa was unaffected.


Conclusion: The comparability and digital nature of AOS images make them suitable for statistical analysis of glycation. Higher glucose concentrations promoted a greater increase in the AOS values of the sections and cells. Moreover, the increase in AOS values varied according to organs and cells, which supports the difference in affected organs among patients with diabetes mellitus. Our findings suggest that a longer hyperglycemic state promotes greater glycation.

Keywords: ultrasound, glycation, advanced glycation end-products, glyceraldehyde, diabetes mellitus, attenuation of sound, microscopy

Article Details

How to Cite
MIURA, Katsutoshi; IWASHITA, Toshihide. Histological Visualization of Glyceraldehyde-Derived Glycation with Glucose using Ultrasound Microscopy. Medical Research Archives, [S.l.], v. 12, n. 2, feb. 2024. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/5035>. Date accessed: 23 nov. 2024. doi: https://doi.org/10.18103/mra.v12i2.5035.
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Issue
Vol 12 No 2 (2024): February issue, Vol.12, Issue 2
Section
Research Articles

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References

1. Monnier VM, Nagaraj RH, Portero-Otin M, et al. IMephrology Dialysis Transplantation Structure of Advanced Maillard Reaction Products and Their Pathological Role.; 1996. https://academic.oup.com/ndt/article/11/supp5/20/1856983
2. Chandel NS. Glycolysis. Cold Spring Harb Perspect Biol. 2021;13(5). doi:10.1101/CSHPERSPECT.A040535
3. Schalkwijk CG, Stehouwer CDA, van Hinsbergh VWM. Fructose-mediated non-enzymatic glycation: Sweet coupling or bad modification. Diabetes Metab Res Rev. 2004;20(5):369-382. doi:10.1002/dmrr.488
4. Vasdev S, Stuckless Bsc J, Adfs H. Role of Methylglyoxal in Essential Hypertension. Vol 19.; 2010.
5. Chellan P, Nagaraj RH. Early glycation products produce pentosidine cross-links on native proteins. Novel mechanism of pentosidine formation and propagation of glycation. Journal of Biological Chemistry. 2001;276(6):3895-3903. doi:10.1074/jbc.M008626200
6. Takeuchi M, Yamagishi S. TAGE (toxic AGEs) hypothesis in various chronic diseases. Med Hypotheses. 2004;63(3):449-452. doi:10.1016/j.mehy.2004.02.042
7. Vyas M, Zuckerman J, Andeen N, Tsang P. PAS (Periodic acid-Schiff). PathologyOutlines.com website. Published December 17, 2023. Accessed December 31, 2023. https://www.pathologyoutlines.com/topic/stainspas.html
8. Alsaad KO, Herzenberg AM. Distinguishing diabetic nephropathy from other causes of glomerulosclerosis: An update. J Clin Pathol. 2007;60(1):18-26. doi:10.1136/jcp.2005.035592
9. Saijo Y. Acoustic microscopy: latest developments and applications. Imaging Med. 2009;1(1):47-63. doi:http://dx.doi.org/10.2217/iim.09.8
10. Azhari H. Appendix A: Typical Acoustic Properties of Tissues. Basics of Biomedical Ultrasound for Engineers. Published online 2010:313-314. doi:10.1002/9780470561478.app1
11. Mast TD. Empirical relationships between acoustic parameters in human soft tissues. Acoustic Research Letters Online. 2000;1(November 2000):37-42. doi:10.1121/1.1336896
12. Miura K, Yamamoto S. Histological imaging from speed-of-sound through tissues by scanning acoustic microscopy (SAM). Protoc Exch. Published online 2013. doi:10.1038/protex.2013.040
13. Tamura K, Ito K, Yoshida S, Mamou J, Miura K, Yamamoto S. Alteration of speed-of-sound by fixatives and tissue processing methods in scanning acoustic microscopy. Front Phys. 2023;11. doi:10.3389/fphy.2023.1060296
14. Hozumi N, Yamashita R, Lee CK, et al. Time-frequency analysis for pulse driven ultrasonic microscopy for biological tissue characterization. In: Ultrasonics. Vol 42. ; 2004:717-722. doi:10.1016/j.ultras.2003.11.005
15. Rask-Madsen C, King GL. Vascular complications of diabetes: Mechanisms of injury and protective factors. Cell Metab. 2013;17(1):20-33. doi:10.1016/j.cmet.2012.11.012
16. Creager MA, Lüscher TF, Cosentino F, Beckman JA. Diabetes and vascular disease. Pathophysiology, clinical consequences, and medical therapy: Part I. Circulation. 2003;108(12):1527-1532. doi:10.1161/01.CIR.0000091257.27563.32
17. Miura K, Iwashita T. Observations of amyloid breakdown by proteases over time using scanning acoustic microscopy. Sci Rep. 2023;13(1):20642. doi:10.1038/s41598-023-48033-4
18. Perrone A, Giovino A, Benny J, Martinelli F. Advanced Glycation End Products (AGEs): Biochemistry, Signaling, Analytical Methods, and Epigenetic Effects. Oxid Med Cell Longev. 2020;2020. doi:10.1155/2020/3818196
19. Nagai R, Shirakawa J ichi, Ohno R ichi, et al. Antibody-based detection of advanced glycation end-products: promises vs. limitations. Glycoconj J. 2016;33(4):545-552. doi:10.1007/s10719-016-9708-9
20. Crisan M, Taulescu M, Crisan D, et al. Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans. PLoS One. 2013;8(10). doi:10.1371/journal.pone.0075003