Early Consequences of Thiophosphate Substitution in Escherichia coli: Possible Epigenetic and Energetic Factors

Article Sidebar

PDF Download
Published Feb 28, 2024
DOI: https://doi.org/10.18103/mra.v12i2.5089

Downloads

Download data is not yet available.

Submit your own article

Register as an author to reserve your spot in the next issue of the Medical Research Archives.

Author Registration

Join the Society

The European Society of Medicine is more than a professional association. We are a community. Our members work in countries across the globe, yet are united by a common goal: to promote health and health equity, around the world.

Join Europe’s leading medical society and discover the many advantages of membership, including free article publication.

Membership

Main Article Content

Elizabeth G. Frayne, PhD
College of General Studies, University of Phoenix, Phoenix, AZ

Abstract

Substitution of thiophosphate for phosphate during the culture of Escherichia coli leads to an increase in biosynthetic activity across a broad spectrum of pathways. To investigate the basis for this enhanced profile, early analysis of RNA transcripts was performed using RNA seq methods. The results identified a possible mediator of activation, namely the ser/thr kinase, yegI, which was increased ~190X fold during the first 1 hr. of induction. There is also a massive shift in the amount of non-ribosomal RNA relative to rRNA of 8-10X fold. RNA seq analysis further supports the idea that energy savings from reduced RNA turnover in thiophosphate treated cells, is the driving force for triggering the enhanced transcriptional profile in Escherichia coli. Thus, in response to an increased energy state, the cells appear to activate a new transcriptional program via a protein kinase cascade.

Article Details

How to Cite
FRAYNE, Elizabeth G.. Early Consequences of Thiophosphate Substitution in Escherichia coli: Possible Epigenetic and Energetic Factors. Medical Research Archives, [S.l.], v. 12, n. 2, feb. 2024. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/5089>. Date accessed: 27 apr. 2024. doi: https://doi.org/10.18103/mra.v12i2.5089.
ABNT APA BibTeX CBE EndNote - EndNote format (Macintosh & Windows) MLA ProCite - RIS format (Macintosh & Windows) RefWorks Reference Manager - RIS format (Windows only) Turabian
Issue
Vol 12 No 2 (2024): February issue, Vol.12, Issue 2
Section
Research Articles

The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.

 

References

1. Frayne EG. Global profile changes in transcripts induced with a phosphate analogue: implications for gene regulation. Molecular and Cellular Biochemistry. 2020;468(1-2):111. doi:10.1007/s11010-020-03715-9
2. Frayne E. Metabolic Regulation of Cell Differentiation. Advanced Chemicobiology Research. Published online November 4, 2020:1-4. doi:https://doi.org/10.37256/acbr.112022543
3. Nowak-Stępniowska A, Osuchowska PN, Fiedorowicz H, Trafny EA. Insight in Hypoxia-Mimetic Agents as Potential Tools for Mesenchymal Stem Cell Priming in Regenerative Medicine. Stem Cells International. March 2022:1-24. doi:10.1155/2022/8775591
4. Yu J-Z, Wang J, Sheridan SD, Perlis RH, Rasenick MM. N-3 polyunsaturated fatty acids promote astrocyte differentiation and neurotrophin production independent of cAMP in patient-derived neural stem cells. Molecular Psychiatry. 2021;26(9):4605. doi:10.1038/s41380-020-0786-5
5. Persad KL, Lopaschuk GD. Energy Metabolism on Mitochondrial Maturation and Its Effects on Cardiomyocyte Cell Fate. Front Cell Dev Biol. 2022;10:886393. Published 2022 Jul 5. doi:10.3389/fcell.2022.886393
6. Agilent Technologies Agilent High Sensitivity RNA ScreenTape System Quick Guide. Accessed January 5, 2024. https://www.agilent.com/cs/library/usermanuals/public/ScreenTape_HSRNA_QG.pdf
7. Keseler IM, Mackie A, Santos-Zavaleta A, et al. The EcoCyc database: reflecting new knowledge about Escherichia coli K-12. Nucleic acids research. 2017;45(D1):D543-D550. doi:10.1093/nar/gkw1003
8. Kuznetsov A, Bollin CJ. NCBI Genome Workbench: Desktop Software for Comparative Genomics, Visualization, and GenBank Data Submission. Methods in Molecular Biology. Published online December 9, 2020:261-295. doi:https://doi.org/10.1007/978-1-0716-1036-7_16
9. Taniguchi Y, Choi PJ, Li GW, et al. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells [published correction appears in Science. 2011 Oct 28;334(6055):453]. Science. 2010;329(5991):533-538. doi:10.1126/science.1188308
10. Hudson CM, Williams KP. The tmRNA website. Nucleic Acids Research. 2014;43(D1):D138-D140. doi:https://doi.org/10.1093/nar/gku1109
11. Rajagopalan K, Dworkin J. Escherichia coli YegI is a novel Ser/Thr kinase lacking conserved motifs that localizes to the inner membrane. FEBS Letters. 2020;594(21):3530-3541. doi:https://doi.org/10.1002/1873-3468.13920
12. Nagarajan SN, Lenoir C, Grangeasse C. Recent advances in bacterial signaling by serine/threonine protein kinases. Trends in Microbiology. 2022;30(6):553-566. doi:10.1016/j.tim.2021.11.005
13. Sánchez-Romero MA, Casadesús J. The bacterial epigenome. Nature Reviews Microbiology. 2019;18(1):7-20. doi:https://doi.org/10.1038/s41579-019-0286-2
14. Liu Q, Lin B, Tao Y. Improved methylation in E. coli via an efficient methyl supply system driven by betaine. Metabolic Engineering. 2022;72:46-55. doi:https://doi.org/10.1016/j.ymben.2022.02.004
15. Bernstein JA, Khodursky AB, Lin PH ., Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proceedings of the National Academy of Sciences. 2002;99(15):9697-9702. doi:https://doi.org/10.1073/pnas.112318199