Characterization of cannabis plant-derived extracellular vesicles for biomedical applications

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Published Oct 28, 2024
DOI: https://doi.org/10.18103/mra.v12i10.5405

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Main Article Content

Qiana L. Matthews
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Ivy N. Thweatt
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Sandani V.T. Wijerathne
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Bernard B. Efa
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Chioma C. Ezeuko
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Ayodeji O. Ipinmoroti
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Rachana Pandit
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Junhuan Xu
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Kelvin A. Fluker
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Olufemi Ajayi
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA

Abstract

The scientific interest in cannabis plants is continuously growing, with heightened interest in properties of plant-derived extracellular vesicles. This manuscript focuses on isolating and characterizing vesicles originating from cannabis plants. Establishing the most appropriate and efficient isolation procedure for plant vesicles remains a challenge due to vast differences in the physio-structural characteristics of different plant cultivars within the same species, and different species within the same genera. In this study, we employed a crude but standard isolation procedure for the extraction of apoplastic wash fluid, which is known to contain plant-derived extracellular vesicles. This method includes a detailed stepwise process of plant-derived extracellular vesicles extraction from two (2) cultivars of cannabis plants, namely: Citrus and BaOx. Approximately, 150 leaves were collected from each plant strain. In order to collect plant-derived extracellular vesicles pellets, apoplastic wash fluid was extracted from the plants via negative pressure permeabilization and infiltration followed by high-speed differential ultracentrifugation. Apoplastic wash fluid fractions were collected for Citrus and BaOx fractions, P100 and P40. Particle tracking analysis of plant-derived extracellular vesicles revealed particle sizes ranging from 60 to 160 nanometers. Both cultivar fractions yielded high levels of plant-derived extracellular vesicles, and contained enriched plant-derived extracellular vesicles RNA levels. Our results suggest that the cannabis apoplastic wash fluid fractions (P100 and P40) yielded plant-derived extracellular vesicles. In total, the results provide a guide for the selection and optimization of cannabis-derived extracellular vesicles. Subsequently, these cannabis plant-derived vesicles can be used for further biomedical uses.

Keywords: Plant-derived extracellular vesicles, Cannabis, Cannabidiol, Cultivar, Apoplastic wash fluid, Extracellular vesicles

Article Details

How to Cite
MATTHEWS, Qiana L. et al. Characterization of cannabis plant-derived extracellular vesicles for biomedical applications. Medical Research Archives, [S.l.], v. 12, n. 10, oct. 2024. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/5405>. Date accessed: 22 dec. 2024. doi: https://doi.org/10.18103/mra.v12i10.5405.
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Issue
Vol 12 No 10 (2024): October Issue, Issue 10, VOl.12
Section
Research Articles

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References

1. https://www.govinfo.gov/app/details/PLAW-115publ334. Agriculture Improvement Act of 2018:.
2. https://www.govinfo.gov/app/details/PLAW-113publ79. Agriculture Act of 2014.
3. Fike J. Industrial Hemp: Renewed Opportunities for an Ancient Crop. Critical Reviews in Plant Sciences. 2016;35(5-6):406-424.
4. Fordjour E, Manful CF, Sey AA, et al. Cannabis: a multifaceted plant with endless potentials. Frontiers in pharmacology. 2023;14:1200269. doi:10.3389/fphar.2023.1200269
5. Small E. Evolution and Classification of Cannabis sativa (Marijuana, Hemp) in Relation to Human Utilization. Botanical Review. 08/19 2015;81:189-294. doi:10.1007/s12229-015-9157-3
6. Bridgeman MB, Abazia DT. Medicinal Cannabis: History, Pharmacology, And Implications for the Acute Care Setting. P & T : a peer-reviewed journal for formulary management. Mar 2017;42(3):180-188.
7. Hillig K. Genetic Evidence for Speciation in Cannabis (Cannabaceae). Genetic Resources and Crop Evolution. 01/03 2005;52:161-180. doi:10.1007/s10722-003-4452-y
8. Corsato Alvarenga I, Panickar KS, Hess H, McGrath S. Scientific Validation of Cannabidiol for Management of Dog and Cat Diseases. Annual review of animal biosciences. Feb 15 2023;11:227-246. doi:10.1146/annurev-animal-081122-070236
9. Yu CHJ, Rupasinghe HPV. Cannabidiol-based natural health products for companion animals: Recent advances in the management of anxiety, pain, and inflammation. Research in veterinary science. Nov 2021;140:38-46. doi:10.1016/j.rvsc.2021.08.001
10. Hameed M, Prasad S, Jain E, et al. Medical Cannabis for Chronic Nonmalignant Pain Management. Current pain and headache reports. Apr 2023;27(4):57-63. doi:10.1007/s11916-023-01101-w
11. Hill KP, Palastro MD. Medical cannabis for the treatment of chronic pain and other disorders: misconceptions and facts. Polish archives of internal medicine. Nov 30 2017;127(11):785-789. doi:10.20452/pamw.4123
12. Patel AD. Cannabinoids in Neurologic Illnesses. Neurologic clinics. Feb 2021;39(1):231-241. doi:10.1016/j.ncl.2020.09.012
13. Armour M, Sinclair J, Cheng J, et al. Endometriosis and Cannabis Consumption During the COVID-19 Pandemic: An International Cross-Sectional Survey. Cannabis and cannabinoid research. Aug 2022;7(4):473-481. doi:10.1089/can.2021.0162
14. Moreno-Rius J. The Cerebellum, THC, and Cannabis Addiction: Findings from Animal and Human Studies. Cerebellum (London, England). Jun 2019;18(3):593-604. doi:10.1007/s12311-018-0993-7
15. Suharta S, Barlian A, Hidajah AC, et al. Plant-derived exosome-like nanoparticles: A concise review on its extraction methods, content, bioactivities, and potential as functional food ingredient. Journal of food science. Jul 2021;86(7):2838-2850. doi:10.1111/1750-3841.15787
16. Urzì O, Raimondo S, Alessandro R. Extracellular Vesicles from Plants: Current Knowledge and Open Questions. International journal of molecular sciences. May 20 2021;22(10)doi:10.3390/ijms22105366
17. Huang Y, Wang S, Cai Q, Jin H. Effective methods for isolation and purification of extracellular vesicles from plants. Journal of integrative plant biology. Dec 2021;63(12):2020-2030. doi:10.1111/jipb.13181
18. Alzahrani FA, Khan MI, Kameli N, Alsahafi E, Riza YM. Plant-Derived Extracellular Vesicles and Their Exciting Potential as the Future of Next-Generation Drug Delivery. Biomolecules. May 15 2023;13(5) doi:10.3390/biom13050839
19. Ipinmoroti AO, Turner J, Bellenger EJ, et al. Characterization of cannabis strain-plant-derived extracellular vesicles as potential biomarkers. Protoplasma. Nov 2023;260(6):1603-1606. doi:10.1007/s00709-023-01870-6
20. Pandit R, Ipinmoroti AO, Crenshaw BJ, Li T, Matthews QL. Canine Coronavirus Infection Modulates the Biogenesis and Composition of Cell-Derived Extracellular Vesicles. Biomedicines. Mar 21 2023;11(3)doi:10.3390/biomedicines11030976
21. Kosgodage US, Mould R, Henley AB, et al. Cannabidiol (CBD) Is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer. Frontiers in pharmacology. 2018;9:889. doi:10.3389/fphar.2018.00889
22. Kosgodage US, Uysal-Onganer P, MacLatchy A, et al. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Translational oncology. Mar 2019;12(3):513-522. doi:10.1016/j.tranon.2018.12.004
23. Kumar S, Crenshaw BJ, Williams SD, Bell CR, Matthews QL, Sims B. Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochemical research. Apr 2021;46(4):1006-1018. doi:10.1007/s11064-021-03231-2
24. Jones LB, Kumar S, Curry AJ, et al. Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles. Biomedicines. Sep 30 2019;7(4)doi:10.3390/biomedicines7040078
25. Cho JH, Hong YD, Kim D, et al. Confirmation of plant-derived exosomes as bioactive substances for skin application through comparative analysis of keratinocyte transcriptome. Applied Biological Chemistry. 2022/01/31 2022;65(1):8. doi:10.1186/s13765-022-00676-z
26. Ganesh S, Lam TT, Garcia-Milian R, et al. Peripheral signature of altered synaptic integrity in young onset cannabis use disorder: A proteomic study of circulating extracellular vesicles. The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry. Sep-Oct 2023;24(7):603-613. doi:10.1080/15622975.2023.2197039
27. Tajik T, Baghaei K, Moghadam VE, Farrokhi N, Salami SA. Extracellular vesicles of cannabis with high CBD content induce anticancer signaling in human hepatocellular carcinoma. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. Aug 2022;152:113209. doi:10.1016/j.biopha.2022.113209
28. Kalarikkal SP, Sundaram GM. Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2. Toxicology and applied pharmacology. Mar 1 2021;414:115425. doi:10.1016/j.taap.2021.115425
29. Pomatto MAC, Gai C, Negro F, et al. Oral Delivery of mRNA Vaccine by Plant-Derived Extracellular Vesicle Carriers. Cells. Jul 11 2023;12(14) doi:10.3390/cells12141826
30. Pomatto MAC, Gai C, Negro F, et al. Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine. Pharmaceutics. Mar 17 2023;15(3)doi:10.3390/pharmaceutics15030974
31. Grammenos G, Kouneli V, Mavroeidis A, et al. Beneficial Insects for Biological Pest Control in Greenhouse Cannabis Production. vol 78. 2021:85-93.
32. McPartland jM. A review of Cannabis diseases. Journal of the International Hemp Association 1996;3(1):19-23.
33. McPartland JM. Hemp diseases and pests: management and biological control: an advanced treatise.
34. Schreiner M, ; Cranshaw, W, . A Survey of the Arthropod Fauna Associated with Hemp (Cannabis sativa L.) Grown in Eastern Colorado. Researh. Journal of the Kansas Entomological Society 2020;93(2):113-131.
35. Hirsch KLaKAK. Two-spotted spider mites in hemp. Alabama Cooperative Extension System. ANR-2850.