Characterization of cannabis plant-derived extracellular vesicles for biomedical applications

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Published Oct 28, 2024
DOI: https://doi.org/10.18103/mra.v12i10.5405

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Main Article Content

Qiana L. Matthews
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Ivy N. Thweatt
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Sandani V.T. Wijerathne
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Bernard B. Efa
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Chioma C. Ezeuko
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Ayodeji O. Ipinmoroti
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Rachana Pandit
Microbiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA;  Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
Junhuan Xu
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Kelvin A. Fluker
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA
Olufemi Ajayi
Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA; Industrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL 36104, USA

Abstract

The scientific interest in cannabis plants is continuously growing, with heightened interest in properties of plant-derived extracellular vesicles. This manuscript focuses on isolating and characterizing vesicles originating from cannabis plants. Establishing the most appropriate and efficient isolation procedure for plant vesicles remains a challenge due to vast differences in the physio-structural characteristics of different plant cultivars within the same species, and different species within the same genera. In this study, we employed a crude but standard isolation procedure for the extraction of apoplastic wash fluid, which is known to contain plant-derived extracellular vesicles. This method includes a detailed stepwise process of plant-derived extracellular vesicles extraction from two (2) cultivars of cannabis plants, namely: Citrus and BaOx. Approximately, 150 leaves were collected from each plant strain. In order to collect plant-derived extracellular vesicles pellets, apoplastic wash fluid was extracted from the plants via negative pressure permeabilization and infiltration followed by high-speed differential ultracentrifugation. Apoplastic wash fluid fractions were collected for Citrus and BaOx fractions, P100 and P40. Particle tracking analysis of plant-derived extracellular vesicles revealed particle sizes ranging from 60 to 160 nanometers. Both cultivar fractions yielded high levels of plant-derived extracellular vesicles, and contained enriched plant-derived extracellular vesicles RNA levels. Our results suggest that the cannabis apoplastic wash fluid fractions (P100 and P40) yielded plant-derived extracellular vesicles. In total, the results provide a guide for the selection and optimization of cannabis-derived extracellular vesicles. Subsequently, these cannabis plant-derived vesicles can be used for further biomedical uses.

Keywords: Plant-derived extracellular vesicles, Cannabis, Cannabidiol, Cultivar, Apoplastic wash fluid, Extracellular vesicles

Article Details

How to Cite
MATTHEWS, Qiana L. et al. Characterization of cannabis plant-derived extracellular vesicles for biomedical applications. Medical Research Archives, [S.l.], v. 12, n. 10, oct. 2024. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/5405>. Date accessed: 15 nov. 2024. doi: https://doi.org/10.18103/mra.v12i10.5405.
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Issue
Vol 12 No 10 (2024): October Issue, Issue 10, VOl.12
Section
Research Articles

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