Plastic embedding tissue for laser microdissection-assisted developmental genomics analyses
Main Article Content
Abstract
The analysis of nucleic acids from single cells functioning in their multicellular context is an important part of performing robust developmental genomics studies. Toward this goal, collecting the desired cell(s) from their milieu has been aided by physical means such as laser microdissection. However, tissue processing for laser microdissection has the potential disadvantage of losing molecular information. Procedures involving tissue embedding in plastic have the potential to circumvent these disadvantages, permitting the robust analysis of nucleic acids from single cells isolated from complex tissues.
Article Details
How to Cite
KLINK, Vincent.
Plastic embedding tissue for laser microdissection-assisted developmental genomics analyses.
Medical Research Archives, [S.l.], v. 4, n. 5, sep. 2016.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/575>. Date accessed: 26 dec. 2024.
Keywords
Laser microdissection
Section
Review Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
REFERENCES
Arnold WH, Gaengler P, Kalkutschke L. 1998. Three-dimensional reconstruction of approximal subsurface caries lesions in deciduous molars. Clin Oral Investig 2: 174-179
Arnold WH, Konopka S, Kriwalsky MS, Gaengler P. 2003. Morphological analysis and chemical content of natural dentin carious lesion zones. Ann Anat 185: 419-424
Bako P, Bassiouni M, Eckhard A, Gerlinger I, Frick C, Löwenheim H, Müller M. 2015. Methyl methacrylate embedding to study the morphology and immunohistochemistry of adult guinea pig and mouse cochleae. J Neurosci Methods 254: 86-93
Bernsen MR, Dijkman HB, de Vries E, Figdor CG, Ruiter DJ, Adema GJ, van Muijen GN. 1998. Identification of multiple mRNA and DNA sequences from small tissue samples isolated by laser-assisted microdissection. Lab Invest 78: 1267-1273
Bhattacharya SH, Gal AA, Murray KK. 2003. Laser capture microdissection MALDI for direct analysis of archival tissue. J Proteome Res 2: 95-98
Biase FH, Everts RE, Oliveira R, Santos-Biase WK, Fonseca Merighe GK, Smith LC, Martelli L, Lewin H, Meirelles FV. 2014. Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage. Zygote 22: 69-79
Brorson SH, Reinholt FP. 2008.The intensity of immunogold labeling of deplasticized acrylic sections compared to deplasticized epoxy sections-Theoretical deductions and experimental data. Micron 39: 144-150
Cadwell CR, Palasantza A, Jiang X, Berens P, Deng Q, Yilmaz M, Reimer J, Shen S, Bethge M, Tolias KF, Sandberg R, Tolias AS. 2016. Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq. Nat Biotechnol 34: 199-203
Cancer Genome Atlas Research Network. 2011. Integrated genomic analyses of ovarian carcinoma. Nature 474: 609-615
Cullen TS. 1895. A rapid method of making permanent sections from frozen sections by the use of formalin. Johns Hopkins Hosp Bull 6: 67
Dalmas DA, Scicchitano MS, Chen Y, Kane J, Mirabile R, Schwartz LW, Thomas HC, Boyce RW. 2008. Transcriptional profiling of laser capture microdissected rat arterial elements: fenoldopam-induced vascular toxicity as a model system. Toxicol Pathol 36: 496-519
Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA. 1996. Laser capture microdissection. Science 274: 998–1001
Emmert-Buck MR, Gillespie JW, Paweletz CP, Ornstein DK, Basrur V, Appella E, Wang QH, Huang J, Hu N, Taylor P, Petricoin EF 3rd. 2000. An approach to proteomic analysis of human tumors. Mol Carcinog 27: 158-165
Fulwyler MJ. 1965. Electronic separation of biological cells by volume. Science 150: 910–911
Fuzik J, Zeisel A, Máté Z, Calvigioni D, Yanagawa Y, Szabó G, Linnarsson S, Harkany T. 2016. Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes. Nat Biotechnol 34: 175-183
Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, Maronpot RR. 1999. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinog 25: 86-91
Hedrum A, Pontén F, Ren Z, Lundeberg J, Pontén J, Uhlén M. 1994. Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. Biotechniques 17, 118-119, 122-124, 126-129
Isenberg G., Bielser W., Meier-Ruge W., Remy E. 1976. Cell surgery by laser micro-dissection: a preparative method. J Microsc 107: 19–24
Ishii T, Kasama K, Kondo M, Takahash T. 1993. Cryostat Sectioning of Formalin-Fixed Brain: Further Attempt to Improve Section Quality by Previous Infiltration with O.C.T. Compound. Tohoku J Exp Med 171: 101-105
Jessani N, Humphrey M, McDonald WH, Niessen S, Masuda K, Gangadharan B, Yates JR 3rd, Mueller BM, Cravatt BF. 2004. Carcinoma and stromal enzyme activity profiles associated with breast tumor growth in vivo. Proc Natl Acad Sci USA 101: 13756–13761
Jessani N, Liu Y, Humphrey M, Cravatt BF. 2002. Enyzme activity profiles of the secreted and membrane proteome that depict cancer cell invasiveness. Proc Natl Acad Sci USA 99: 10335–10340
Jang JS, Simon VA, Feddersen RM, Rakhshan F, Schultz DA, Zschunke MA, Lingle WL, Kolbert CP, Jen J. 2011. Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays. BMC Genomics 12: 144. doi: 10.1186/1471-2164-12-144
Jonsson N, Lagerstedt S. 1957. Demonstration of ribonuclease activity in sections from carnoy fixed rat pancreas. Experientia 13: 321-323
Jonsson N, Lagerstedt S. 1958. Losses of nucleic acid derivatives from fixed tissue during flattening of paraffin sections on water. Experientia 14: 157-159
Julius MH, Masuda T, Herzenberg LA. 1972. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci USA 69: 1934–1938
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2011. Differences in gene expression amplitude overlie a conserved transcriptomic program occurring between the rapid and potent localized resistant reaction at the syncytium of the Glycine max genotype Peking (PI 548402) as compared to the prolonged and potent resistant reaction of PI 88788. Plant Molecular Biology 75: 141-165
Klink VP, Overall CC, Alkharouf NW, MacDonald MH, Matthews BF. 2010b. Microarray detection calls as a means to compare transcripts expressed within syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by the soybean cyst nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology 1-30
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2010a Syncytium gene expression in Glycine max[PI 88788] roots undergoing a resistant reaction to the parasitic nematode Heterodera glycines. Plant Physiology and Biochemistry 48: 176-193
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2009. A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode). Plant Molecular Biology 71: 525-567
Klink VP, Overall CC, Alkharouf N, MacDonald MH, Matthews BF. 2007. Laser capture microdissection (LCM) and comparative microarray expression analysis of syncytial cells isolated from incompatible and compatible soybean roots infected by soybean cyst nematode (Heterodera glycines). Planta 226: 1389-1409
Klink VP, Alkharouf N, MacDonald M, Matthews BF. 2005. Laser capture microdissection (LCM) and analysis of Glycine max (soybean) syncytial cells formed by the soybean cyst nematode Heterodera glycines. Plant Molecular Biology 59: 969-983
Lin DM, Loveall B, Ewer J, Deitcher DL, Sucher NJ. 2007. Characterization of mRNA expression in single neurons. Methods Mol Biol 399: 133-152
Matsye PD, Kumar R, Hosseini P, Jones CM, Tremblay A, Alkharouf NW, Matthews BF, Klink VP. 2011. Mapping cell fate decisions that occur during soybean defense responses. Plant Molecular Biology 77: 513-528
Matsye PD, Lawrence GW, Youssef RM, Kim K-H, Matthews BF, Lawrence KS, Klink VP. 2012. The expression of a naturally occurring, truncated allele of an -SNAP gene suppresses plant parasitic nematode infection. Plant Molecular Biology 80: 131-155
Meier-Ruge W, Bielser W, Remy E, Hillenkamp F, Nitsche R, Unsold R. 1976. The laser in the Lowry technique for microdissection of freeze-dried tissue slices. Histochem J 8: 387-401
Nakazono M, Qiu F, Borsuk LA, Schnable PS. 2003. Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell. 15: 583–596
Nair KK. 1958. The effect of some common fixatives on the enzymatic activity of ribonuclease. Experientia 14: 172-173
Nishimoto K, Rigsby CS, Wang T, Mukai K, Gomez-Sanchez CE, Rainey WE, Seki T. 2012. Transcriptome analysis reveals differentially expressed transcripts in rat adrenal zona glomerulosa and zona fasciculata. Endocrinology 153: 1755-1763
Nonn L, Vaishnav A, Gallagher L, Gann PH. 2010. mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials. Exp Mol Pathol 88: 45-51
Ornstein DK, Gillespie JW, Paweletz CP, Duray PH, Herring J, Vocke CD, Topalian SL, Bostwick DG, Linehan WM, Petricoin EF 3rd, Emmert-Buck MR. 2000. Proteomic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines. Electrophoresis 21: 2235-2242
Pant SR, Matsye PD, McNeece BT, Sharma K, Krishnavajhala A, Lawrence GW, Klink VP. 2014. Syntaxin 31 functions in Glycine max resistance to the plant parasitic nematode Heterodera glycines Plant Molecular Biology 85: 107–121
Pollen AA, Nowakowski TJ, Shuga J, Wang X, Leyrat AA, Lui JH, Li N3, Szpankowski L, Fowler B, Chen P, Ramalingam N, Sun G, Thu M, Norris M, Lebofsky R, Toppani D, Kemp DW 2nd, Wong M, Clerkson B, Jones BN, Wu S, Knutsson L, Alvarado B, Wang J, Weaver LS, May AP, Jones RC, Unger MA, Kriegstein AR, West JA. 2014. Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex. Nat Biotechnol 32: 1053-1058
Pollen AA, Nowakowski TJ, Chen J, Retallack H, Sandoval-Espinosa C, Nicholas CR, Shuga J, Liu SJ, Oldham MC, Diaz A, Lim DA, Leyrat AA, West JA, Kriegstein AR. 2015. Molecular identity of human outer radial glia during cortical development. Cell 163: 55-67
Pusey W. 1974. Paraffin embedding after O.C.T. Stain Technol 49: 410-11
Schad M, Mungur R, Fiehn O, Kehr J. 2005. Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana. Plant Methods 1: 2
Schütze K, Lahr G. 1998. Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16: 737-742
Schwach V, Passier R. 2016. Generation and purification of human stem cell-derived cardiomyocytes. Differentiation 91: 126-138
Sgroi DC, Teng S, Robinson G, LeVangie R, Hudson JR Jr, Elkahloun AG. 1999. In vivo gene expression profile analysis of human breast cancer progression. Cancer Res 59: 5656-5661
Sharma K, Pant SR, McNeece BT, Lawrence GW, Klink VP. 2016. Co-regulation of the Glycine max soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE)-containing regulon occurs during defense to a root pathogen. Journal of Plant Interactions 11: 74–93
Singhrao SK, Müller CT, Gilbert SJ, Duance VC, Archer CW. 2009. An immunofluorescence method for postembedded tissue in the acrylic resin Technovit 9100 New using fluorescein isothiocyanate secondary detection. Microsc Res Tech 72: 501-506
Steiniger BS, Bubel S, Böckler W, Lampp K, Seiler A, Jablonski B, Guthe M, Stachniss V. 2013. Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100. Cells Tissues Organs 198: 57-65
Sturm D, Marselli L, Ehehalt F, Richter D, Distler M, Kersting S, Grützmann R, Bokvist K, Froguel P, Liechti R, Jörns A, Meda P, Baretton GB, Saeger HD, Schulte AM, Marchetti P, Solimena M. 2013. Improved protocol for laser microdissection of human pancreatic islets from surgical specimens. J Vis Exp 2013 Jan 6;(71). pii: 50231 doi:10.3791/50231
Tadros Y, Ruiz-Deya G, Crawford BE, Thomas R, Abdel-Mageed AB. 2003. In vivo proteomic analysis of cytokine expression in laser capture-microdissected urothelial cells of obstructed ureteropelvic junction procured by laparoscopic dismembered pyeloplasty. J Endourol 17: 333-336
Tam AS, Foley JF, Devereux TR, Maronpot RR Massey TE. 1999. High frequency and heterogeneous distribution of p53 mutations in aflatoxin B1-induced mouse lung tumors. Cancer Res 59: 3634-3640
Weaver JM, Ross-Innes CS, Shannon N, Lynch AG, Forshew T, Barbera M, Murtaza M, Ong CA, Lao-Sirieix P, Dunning MJ, Smith L, Smith ML, Anderson CL, Carvalho B, O'Donovan M, Underwood TJ, May AP, Grehan N, Hardwick R, Davies J, Oloumi A, Aparicio S, Caldas C, Eldridge MD, Edwards PA, Rosenfeld N, Tavaré S, Fitzgerald RC; OCCAMS Consortium. 2014. Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis. Nat Genet 46: 837-843
Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, Lennon NJ, Livak KJ, Mikkelsen TS, Rinn JL. 2014. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat Biotechnol 32: 381-386
Vertenten G, Vlaminck L, Ducatelle R, Lippens E, Cornelissen M, Gasthuys F. 2008. Immunohistochemical analysis of low-temperature methylmethacrylate resin-embedded goat tissues. Anat Histol Embryol 37: 452-457
Wilson LB. 1905. A method for the rapid preparation of fresh tissues for the microscope. JAMA 45: 1737
Wittenburg G, Volkel C, Mai R, Lauer G. 2009. Immunohistochemical comparison of differentiation markers on paraffin and plastic embedded human bone samples. J Physiol Pharmacol 60 Suppl 8: 43-49
Yang R, Davies CM, Archer CW, Richards RG. 2003. Immunohistochemistry of matrix markers in Technovit 9100 New-embedded undecalcified bone sections. Eur Cell Mater 6: 57-71
Xin Y, Kim J, Ni M, Wei Y, Okamoto H, Lee J, Adler C, Cavino K, Murphy AJ, Yancopoulos GD, Lin HC, Gromada J. 2016. Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. Proc Natl Acad Sci USA 113: 3293-3298
Arnold WH, Gaengler P, Kalkutschke L. 1998. Three-dimensional reconstruction of approximal subsurface caries lesions in deciduous molars. Clin Oral Investig 2: 174-179
Arnold WH, Konopka S, Kriwalsky MS, Gaengler P. 2003. Morphological analysis and chemical content of natural dentin carious lesion zones. Ann Anat 185: 419-424
Bako P, Bassiouni M, Eckhard A, Gerlinger I, Frick C, Löwenheim H, Müller M. 2015. Methyl methacrylate embedding to study the morphology and immunohistochemistry of adult guinea pig and mouse cochleae. J Neurosci Methods 254: 86-93
Bernsen MR, Dijkman HB, de Vries E, Figdor CG, Ruiter DJ, Adema GJ, van Muijen GN. 1998. Identification of multiple mRNA and DNA sequences from small tissue samples isolated by laser-assisted microdissection. Lab Invest 78: 1267-1273
Bhattacharya SH, Gal AA, Murray KK. 2003. Laser capture microdissection MALDI for direct analysis of archival tissue. J Proteome Res 2: 95-98
Biase FH, Everts RE, Oliveira R, Santos-Biase WK, Fonseca Merighe GK, Smith LC, Martelli L, Lewin H, Meirelles FV. 2014. Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage. Zygote 22: 69-79
Brorson SH, Reinholt FP. 2008.The intensity of immunogold labeling of deplasticized acrylic sections compared to deplasticized epoxy sections-Theoretical deductions and experimental data. Micron 39: 144-150
Cadwell CR, Palasantza A, Jiang X, Berens P, Deng Q, Yilmaz M, Reimer J, Shen S, Bethge M, Tolias KF, Sandberg R, Tolias AS. 2016. Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq. Nat Biotechnol 34: 199-203
Cancer Genome Atlas Research Network. 2011. Integrated genomic analyses of ovarian carcinoma. Nature 474: 609-615
Cullen TS. 1895. A rapid method of making permanent sections from frozen sections by the use of formalin. Johns Hopkins Hosp Bull 6: 67
Dalmas DA, Scicchitano MS, Chen Y, Kane J, Mirabile R, Schwartz LW, Thomas HC, Boyce RW. 2008. Transcriptional profiling of laser capture microdissected rat arterial elements: fenoldopam-induced vascular toxicity as a model system. Toxicol Pathol 36: 496-519
Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA. 1996. Laser capture microdissection. Science 274: 998–1001
Emmert-Buck MR, Gillespie JW, Paweletz CP, Ornstein DK, Basrur V, Appella E, Wang QH, Huang J, Hu N, Taylor P, Petricoin EF 3rd. 2000. An approach to proteomic analysis of human tumors. Mol Carcinog 27: 158-165
Fulwyler MJ. 1965. Electronic separation of biological cells by volume. Science 150: 910–911
Fuzik J, Zeisel A, Máté Z, Calvigioni D, Yanagawa Y, Szabó G, Linnarsson S, Harkany T. 2016. Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes. Nat Biotechnol 34: 175-183
Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, Maronpot RR. 1999. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinog 25: 86-91
Hedrum A, Pontén F, Ren Z, Lundeberg J, Pontén J, Uhlén M. 1994. Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. Biotechniques 17, 118-119, 122-124, 126-129
Isenberg G., Bielser W., Meier-Ruge W., Remy E. 1976. Cell surgery by laser micro-dissection: a preparative method. J Microsc 107: 19–24
Ishii T, Kasama K, Kondo M, Takahash T. 1993. Cryostat Sectioning of Formalin-Fixed Brain: Further Attempt to Improve Section Quality by Previous Infiltration with O.C.T. Compound. Tohoku J Exp Med 171: 101-105
Jessani N, Humphrey M, McDonald WH, Niessen S, Masuda K, Gangadharan B, Yates JR 3rd, Mueller BM, Cravatt BF. 2004. Carcinoma and stromal enzyme activity profiles associated with breast tumor growth in vivo. Proc Natl Acad Sci USA 101: 13756–13761
Jessani N, Liu Y, Humphrey M, Cravatt BF. 2002. Enyzme activity profiles of the secreted and membrane proteome that depict cancer cell invasiveness. Proc Natl Acad Sci USA 99: 10335–10340
Jang JS, Simon VA, Feddersen RM, Rakhshan F, Schultz DA, Zschunke MA, Lingle WL, Kolbert CP, Jen J. 2011. Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays. BMC Genomics 12: 144. doi: 10.1186/1471-2164-12-144
Jonsson N, Lagerstedt S. 1957. Demonstration of ribonuclease activity in sections from carnoy fixed rat pancreas. Experientia 13: 321-323
Jonsson N, Lagerstedt S. 1958. Losses of nucleic acid derivatives from fixed tissue during flattening of paraffin sections on water. Experientia 14: 157-159
Julius MH, Masuda T, Herzenberg LA. 1972. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci USA 69: 1934–1938
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2011. Differences in gene expression amplitude overlie a conserved transcriptomic program occurring between the rapid and potent localized resistant reaction at the syncytium of the Glycine max genotype Peking (PI 548402) as compared to the prolonged and potent resistant reaction of PI 88788. Plant Molecular Biology 75: 141-165
Klink VP, Overall CC, Alkharouf NW, MacDonald MH, Matthews BF. 2010b. Microarray detection calls as a means to compare transcripts expressed within syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by the soybean cyst nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology 1-30
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2010a Syncytium gene expression in Glycine max[PI 88788] roots undergoing a resistant reaction to the parasitic nematode Heterodera glycines. Plant Physiology and Biochemistry 48: 176-193
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF. 2009. A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) genotype PI 548402 (Peking) undergoing a resistant reaction after infection by Heterodera glycines (soybean cyst nematode). Plant Molecular Biology 71: 525-567
Klink VP, Overall CC, Alkharouf N, MacDonald MH, Matthews BF. 2007. Laser capture microdissection (LCM) and comparative microarray expression analysis of syncytial cells isolated from incompatible and compatible soybean roots infected by soybean cyst nematode (Heterodera glycines). Planta 226: 1389-1409
Klink VP, Alkharouf N, MacDonald M, Matthews BF. 2005. Laser capture microdissection (LCM) and analysis of Glycine max (soybean) syncytial cells formed by the soybean cyst nematode Heterodera glycines. Plant Molecular Biology 59: 969-983
Lin DM, Loveall B, Ewer J, Deitcher DL, Sucher NJ. 2007. Characterization of mRNA expression in single neurons. Methods Mol Biol 399: 133-152
Matsye PD, Kumar R, Hosseini P, Jones CM, Tremblay A, Alkharouf NW, Matthews BF, Klink VP. 2011. Mapping cell fate decisions that occur during soybean defense responses. Plant Molecular Biology 77: 513-528
Matsye PD, Lawrence GW, Youssef RM, Kim K-H, Matthews BF, Lawrence KS, Klink VP. 2012. The expression of a naturally occurring, truncated allele of an -SNAP gene suppresses plant parasitic nematode infection. Plant Molecular Biology 80: 131-155
Meier-Ruge W, Bielser W, Remy E, Hillenkamp F, Nitsche R, Unsold R. 1976. The laser in the Lowry technique for microdissection of freeze-dried tissue slices. Histochem J 8: 387-401
Nakazono M, Qiu F, Borsuk LA, Schnable PS. 2003. Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell. 15: 583–596
Nair KK. 1958. The effect of some common fixatives on the enzymatic activity of ribonuclease. Experientia 14: 172-173
Nishimoto K, Rigsby CS, Wang T, Mukai K, Gomez-Sanchez CE, Rainey WE, Seki T. 2012. Transcriptome analysis reveals differentially expressed transcripts in rat adrenal zona glomerulosa and zona fasciculata. Endocrinology 153: 1755-1763
Nonn L, Vaishnav A, Gallagher L, Gann PH. 2010. mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials. Exp Mol Pathol 88: 45-51
Ornstein DK, Gillespie JW, Paweletz CP, Duray PH, Herring J, Vocke CD, Topalian SL, Bostwick DG, Linehan WM, Petricoin EF 3rd, Emmert-Buck MR. 2000. Proteomic analysis of laser capture microdissected human prostate cancer and in vitro prostate cell lines. Electrophoresis 21: 2235-2242
Pant SR, Matsye PD, McNeece BT, Sharma K, Krishnavajhala A, Lawrence GW, Klink VP. 2014. Syntaxin 31 functions in Glycine max resistance to the plant parasitic nematode Heterodera glycines Plant Molecular Biology 85: 107–121
Pollen AA, Nowakowski TJ, Shuga J, Wang X, Leyrat AA, Lui JH, Li N3, Szpankowski L, Fowler B, Chen P, Ramalingam N, Sun G, Thu M, Norris M, Lebofsky R, Toppani D, Kemp DW 2nd, Wong M, Clerkson B, Jones BN, Wu S, Knutsson L, Alvarado B, Wang J, Weaver LS, May AP, Jones RC, Unger MA, Kriegstein AR, West JA. 2014. Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex. Nat Biotechnol 32: 1053-1058
Pollen AA, Nowakowski TJ, Chen J, Retallack H, Sandoval-Espinosa C, Nicholas CR, Shuga J, Liu SJ, Oldham MC, Diaz A, Lim DA, Leyrat AA, West JA, Kriegstein AR. 2015. Molecular identity of human outer radial glia during cortical development. Cell 163: 55-67
Pusey W. 1974. Paraffin embedding after O.C.T. Stain Technol 49: 410-11
Schad M, Mungur R, Fiehn O, Kehr J. 2005. Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana. Plant Methods 1: 2
Schütze K, Lahr G. 1998. Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16: 737-742
Schwach V, Passier R. 2016. Generation and purification of human stem cell-derived cardiomyocytes. Differentiation 91: 126-138
Sgroi DC, Teng S, Robinson G, LeVangie R, Hudson JR Jr, Elkahloun AG. 1999. In vivo gene expression profile analysis of human breast cancer progression. Cancer Res 59: 5656-5661
Sharma K, Pant SR, McNeece BT, Lawrence GW, Klink VP. 2016. Co-regulation of the Glycine max soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE)-containing regulon occurs during defense to a root pathogen. Journal of Plant Interactions 11: 74–93
Singhrao SK, Müller CT, Gilbert SJ, Duance VC, Archer CW. 2009. An immunofluorescence method for postembedded tissue in the acrylic resin Technovit 9100 New using fluorescein isothiocyanate secondary detection. Microsc Res Tech 72: 501-506
Steiniger BS, Bubel S, Böckler W, Lampp K, Seiler A, Jablonski B, Guthe M, Stachniss V. 2013. Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100. Cells Tissues Organs 198: 57-65
Sturm D, Marselli L, Ehehalt F, Richter D, Distler M, Kersting S, Grützmann R, Bokvist K, Froguel P, Liechti R, Jörns A, Meda P, Baretton GB, Saeger HD, Schulte AM, Marchetti P, Solimena M. 2013. Improved protocol for laser microdissection of human pancreatic islets from surgical specimens. J Vis Exp 2013 Jan 6;(71). pii: 50231 doi:10.3791/50231
Tadros Y, Ruiz-Deya G, Crawford BE, Thomas R, Abdel-Mageed AB. 2003. In vivo proteomic analysis of cytokine expression in laser capture-microdissected urothelial cells of obstructed ureteropelvic junction procured by laparoscopic dismembered pyeloplasty. J Endourol 17: 333-336
Tam AS, Foley JF, Devereux TR, Maronpot RR Massey TE. 1999. High frequency and heterogeneous distribution of p53 mutations in aflatoxin B1-induced mouse lung tumors. Cancer Res 59: 3634-3640
Weaver JM, Ross-Innes CS, Shannon N, Lynch AG, Forshew T, Barbera M, Murtaza M, Ong CA, Lao-Sirieix P, Dunning MJ, Smith L, Smith ML, Anderson CL, Carvalho B, O'Donovan M, Underwood TJ, May AP, Grehan N, Hardwick R, Davies J, Oloumi A, Aparicio S, Caldas C, Eldridge MD, Edwards PA, Rosenfeld N, Tavaré S, Fitzgerald RC; OCCAMS Consortium. 2014. Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis. Nat Genet 46: 837-843
Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, Lennon NJ, Livak KJ, Mikkelsen TS, Rinn JL. 2014. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat Biotechnol 32: 381-386
Vertenten G, Vlaminck L, Ducatelle R, Lippens E, Cornelissen M, Gasthuys F. 2008. Immunohistochemical analysis of low-temperature methylmethacrylate resin-embedded goat tissues. Anat Histol Embryol 37: 452-457
Wilson LB. 1905. A method for the rapid preparation of fresh tissues for the microscope. JAMA 45: 1737
Wittenburg G, Volkel C, Mai R, Lauer G. 2009. Immunohistochemical comparison of differentiation markers on paraffin and plastic embedded human bone samples. J Physiol Pharmacol 60 Suppl 8: 43-49
Yang R, Davies CM, Archer CW, Richards RG. 2003. Immunohistochemistry of matrix markers in Technovit 9100 New-embedded undecalcified bone sections. Eur Cell Mater 6: 57-71
Xin Y, Kim J, Ni M, Wei Y, Okamoto H, Lee J, Adler C, Cavino K, Murphy AJ, Yancopoulos GD, Lin HC, Gromada J. 2016. Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. Proc Natl Acad Sci USA 113: 3293-3298