Bridging Models: Preeclampsia Biomarkers in Salt-Loaded Rats and Human Patients

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Published Jan 24, 2025
DOI: https://doi.org/10.18103/mra.v13i1.6233

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Main Article Content

Deliana Rojasa
Center for Biophysics and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), AP 21827, Caracas 1020A, Venezuela
Cilia Abad
Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Kralove, Charles University, Akademika Heyrovskeho 1203, Hradec Kralove 500 05, Czech
Sandy Piñero
Center for Biophysics and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), AP 21827, Caracas 1020A, Venezuela
Margrego Piña
Center for Biophysics and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), AP 21827, Caracas 1020A, Venezuela
Fulgencio Proverbio
Center for Biophysics and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), AP 21827, Caracas 1020A, Venezuela
Reinaldo Marín
Center for Biophysics and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), AP 21827, Caracas 1020A, Venezuela

Abstract

Preeclampsia, a pregnancy-specific syndrome with multisystem involvement, significantly contributes to maternal and fetal morbidity and mortality. This study compared physiological, fetal, and placental parameters between preeclamptic women and salt-loaded pregnant rats, an established animal model for preeclampsia. We evaluated lipid peroxidation levels in both placental homogenates and red blood cell ghosts, and Ca-ATPase activity in placental homogenates. Additionally, we assessed the effects of magnesium sulfate (MgSO4) treatment on these parameters.


Salt-loaded pregnant rats received 1.8% NaCl solution ad libitum for seven days starting from the 15th day of pregnancy. For analysis, blood and placental samples were obtained from preeclamptic women and the rat model.


Results showed that salt-loaded pregnant rats exhibited similar characteristics to preeclamptic women, including increased lipid peroxidation, and lowered Ca-ATPase activity in placental and red blood cell ghosts. MgSO4 treatment in preeclamptic women and salt-loaded rats modified Ca-ATPase activity and lipid peroxidation levels in red blood cell membranes and placental membranes, bringing values closer to normal values. The reduction in lipid peroxidation by MgSO4 may account for increased Ca-ATPase activity.


This study validates the salt-loaded pregnant rat model as a valuable tool for investigating preeclampsia, offering insights into the condition's pathophysiology and potential therapeutic interventions.

Keywords: Preeclampsia, Lipid peroxidation, Ca-ATPase, Animal Model, Magnesium Sulfate

Article Details

How to Cite
ROJASA, Deliana et al. Bridging Models: Preeclampsia Biomarkers in Salt-Loaded Rats and Human Patients. Medical Research Archives, [S.l.], v. 13, n. 1, jan. 2025. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/6233>. Date accessed: 10 feb. 2025. doi: https://doi.org/10.18103/mra.v13i1.6233.
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Issue
Vol 13 No 1 (2025): Vol.13 issue 1 January 2025
Section
Research Articles

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