Microneedling and injectable-Platelet Rich Fibrin for Skin Rejuvenation and Regeneration
Main Article Content
Abstract
Aim: The aim of this systematic review is to evaluate the efficacy of microneedling treatment with injectable-platelet-rich fibrin (i-PRF) for facial skin rejuvenation applications.
Methods: The search approach involved the exploration of electronic databases with an advanced search option applied from April 2021 to April 2025 according to our previous study Vesala et al. We performed a search on Medline, Scopus, Embase, and Web of Science. Our online search conducted according to the following PICO framework: (microneedling) AND (or) OR (needling) OR (face rejuvenation) OR (skin rejuvenation) OR (facial regeneration) AND (injectable platelet-rich fibrin) OR (i-PRF).
Results: The search yielded 2 studies. Both proving that the combination of microneedling and injectable-Platelet Rich Fibrin enhances the patients' skin texture, color, elasticity, accompanied by a visible reduction in the appearance of soft wrinkles and also leads to the improvement of acne scars.
Conclusion: The findings from these studies have been systematically reviewed, offering a comprehensive understanding of its efficacy and safety profile. Overall, the findings from existing studies are promising; therefore, there is a pressing need for further research to provide more robust evidence and validate the observed outcomes.
Article Details
How to Cite
VESALA, Anna-Maria; NACOPOULOS, Cleopatra.
Microneedling and injectable-Platelet Rich Fibrin for Skin Rejuvenation and Regeneration.
Medical Research Archives, [S.l.], v. 13, n. 5, may 2025.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/6578>. Date accessed: 23 june 2025.
doi: https://doi.org/10.18103/mra.v13i5.6578.
Section
Review Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
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9. Gainza, G.; Villullas, S.; Pedraz, J. L.; Hernandez, R. M.; Igartua, M. Advances in drug delivery systems (DDSs) to release growth factors for wound healing and skin regeneration. Nanomedicine 2015, 11 (6), 1551-1573. DOI: 10.1016/j.nano.2015.03.002 From NLM Medline.
10. Addis, R.; Cruciani, S.; Santaniello, S.; Bellu, E.; Sarais, G.; Ventura, C.; Maioli, M.; Pintore, G. Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome. Int J Med Sci 2020, 17 (8), 1030-1042. DOI: 10.7150/ijms.43986 From NLM Medline.
11. Oda, Y.; Bikle, D. D. Vitamin D and calcium signaling in epidermal stem cells and their regeneration. World J Stem Cells 2020, 12 (7), 604-611. DOI: 10.4252/wjsc.v12.i7.604 From NLM PubMed-not-MEDLINE.
12. Maisel-Campbell, A. L.; Ismail, A.; Reynolds, K. A.; Poon, E.; Serrano, L.; Grushchak, S.; Farid, C.; West, D. P.; Alam, M. A systematic review of the safety and effectiveness of platelet-rich plasma (PRP) for skin aging. Arch Dermatol Res 2020, 312 (5), 301-315. DOI: 10.1007/s00403-019-01999-6 From NLM Medline.
13. Dhurat, R.; Sukesh, M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective. J Cutan Aesthet Surg 2014, 7 (4), 189-197. DOI: 10.4103/0974-2077.150734 From NLM PubMed-not-MEDLINE.
14. Miron, R. J.; Zucchelli, G.; Pikos, M. A.; Salama, M.; Lee, S.; Guillemette, V.; Fujioka-Kobayashi, M.; Bishara, M.; Zhang, Y.; Wang, H. L.; et al. Use of platelet-rich fibrin in regenerative dentistry: a systematic review. Clin Oral Investig 2017, 21 (6), 1913-1927. DOI: 10.1007/s00784-017-2133-z.
15. Dohan, D. M.; Choukroun, J.; Diss, A.; Dohan, S. L.; Dohan, A. J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006, 101 (3), e37-44. DOI: 10.1016/j.tripleo.2005.07.008.
16. Dohan, D. M.; Choukroun, J.; Diss, A.; Dohan, S. L.; Dohan, A. J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006, 101 (3), e45-50. DOI: 10.1016/j.tripleo.2005.07.009.
17. Gawdat, H. I.; Hegazy, R. A.; Fawzy, M. M.; Fathy, M. Autologous platelet rich plasma: topical versus intradermal after fractional ablative carbon dioxide laser treatment of atrophic acne scars. Dermatol Surg 2014, 40 (2), 152-161. DOI: 10.1111/dsu.12392 From NLM Medline.
18. Tuknayat, A.; Bhalla, M.; Thami, G. P. Platelet-rich plasma is a promising therapy for melasma. J Cosmet Dermatol 2021, 20 (8), 2431-2436. DOI: 10.1111/jocd.14229 From NLM Medline.
19. Na, J. I.; Choi, J. W.; Choi, H. R.; Jeong, J. B.; Park, K. C.; Youn, S. W.; Huh, C. H. Rapid healing and reduced erythema after ablative fractional carbon dioxide laser resurfacing combined with the application of autologous platelet-rich plasma. Dermatol Surg 2011, 37 (4), 463-468. DOI: 10.1111/j.1524-4725.2011.01916.x From NLM Medline.
20. Nacopoulos, C.; Vesala, A. M. Lower facial regeneration with a combination of platelet-rich fibrin liquid matrices based on the low speed centrifugation concept-Cleopatra technique. J Cosmet Dermatol 2020, 19 (1), 185-189. DOI: 10.1111/jocd.13196.
21. Sclafani, A. P.; Azzi, J. Platelet Preparations for Use in Facial Rejuvenation and Wound Healing: A Critical Review of Current Literature. Aesthetic Plast Surg 2015, 39 (4), 495-505. DOI: 10.1007/s00266-015-0504-x From NLM Medline.
22. Kim, E. K.; Kim, H. O.; Park, Y. M.; Park, C. J.; Yu, D. S.; Lee, J. Y. Prevalence and risk factors of depression in geriatric patients with dermatological diseases. Ann Dermatol 2013, 25 (3), 278-284. DOI: 10.5021/ad.2013.25.3.278 From NLM PubMed-not-MEDLINE.
23. Nacopoulos, C.; Gkouskou, K.; Karypidis, D.; Vlastos, I.; Vesala, A. M.; Choukroun, J.; Miron, R. J.; Prokopakis, E. Telomere length and genetic variations affecting telomere length as biomarkers for facial regeneration with platelet-rich fibrin based on the low-speed centrifugation concept. J Cosmet Dermatol 2019, 18 (1), 408-413. DOI: 10.1111/jocd.12666.
24. Trommer, H.; Neubert, R. H. Overcoming the stratum corneum: the modulation of skin penetration. A review. Skin Pharmacol Physiol 2006, 19 (2), 106-121. DOI: 10.1159/000091978 From NLM Medline.
25. Batisse, D.; Bazin, R.; Baldeweck, T.; Querleux, B.; Leveque, J. L. Influence of age on the wrinkling capacities of skin. Skin Res Technol 2002, 8 (3), 148-154. DOI: 10.1034/j.1600-0846.2002.10308.x From NLM Medline.
26. Dsouza, L.; Ghate, V. M.; Lewis, S. A. Derma rollers in therapy: the transition from cosmetics to transdermal drug delivery. Biomed Microdevices 2020, 22 (4), 77. DOI: 10.1007/s10544-020-00530-3.
27. Fernandes, D. Minimally invasive percutaneous collagen induction. Oral Maxillofac Surg Clin North Am 2005, 17 (1), 51-63, vi. DOI: 10.1016/j.coms.2004.09.004.
28. Singh, A.; Yadav, S. Microneedling: Advances and widening horizons. Indian Dermatol Online J 2016, 7 (4), 244-254. DOI: 10.4103/2229-5178.185468.
29. Vesala, A.-M.; Nacopoulos, C.; Gkouskou, K.; Amenta, F.; Ruga, E. Microneedling with injectable platelet-rich fibrin for facial rejuvenation. Plastic and Aesthetic Research 2021, 8 (0), 53.
30. Vesala, A.-M.; Nacopoulos, C.; Karypidis, D.; Ruga, E. Efficacy of microneedling with injectable platelet-rich fibrin (i-PRF) for facial skin rejuvenation. International Journal of Dermatology and Venereology 2023, Publish Ahead of Print, 10.1097/JD1099.0000000000000301. DOI: 10.1097/jd9.0000000000000301.
31. Krishnegowda, R.; Pradhan, S. N.; Belgaumkar, V. A. A Split-Face Study to Evaluate Efficacy of Autologous Injectable Platelet-Rich Fibrin With Microneedling Against Microneedling With Normal Saline (Placebo Control) in Atrophic Acne Scars. Dermatologic Surgery 2023, 49 (10), 938-942. DOI: 10.1097/dss.0000000000003893.
32. Wang, X.; Zhang, Y.; Choukroun, J.; Ghanaati, S.; Miron, R. J. Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP. Int J Mol Sci 2017, 18 (2). DOI: 10.3390/ijms18020331.
33. Miron, R. J.; Fujioka-Kobayashi, M.; Bishara, M.; Zhang, Y.; Hernandez, M.; Choukroun, J. Platelet-Rich Fibrin and Soft Tissue Wound Healing: A Systematic Review. Tissue Eng Part B Rev 2017, 23 (1), 83-99. DOI: 10.1089/ten.TEB.2016.0233.
34. Kubesch, A.; Barbeck, M.; Al-Maawi, S.; Orlowska, A.; Booms, P. F.; Sader, R. A.; Miron, R. J.; Kirkpatrick, C. J.; Choukroun, J.; Ghanaati, S. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo. Platelets 2019, 30 (3), 329-340. DOI: 10.1080/09537104.2018.1445835.
35. Wang, X.; Yang, Y.; Zhang, Y.; Miron, R. J. Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma. J Cosmet Dermatol 2019, 18 (6), 2004-2010. DOI: 10.1111/jocd.12955 From NLM Medline.
36. Choukroun, J.; Ghanaati, S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 2018, 44 (1), 87-95. DOI: 10.1007/s00068-017-0767-9.
2. Sen, C. K.; Gordillo, G. M.; Roy, S.; Kirsner, R.; Lambert, L.; Hunt, T. K.; Gottrup, F.; Gurtner, G. C.; Longaker, M. T. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen 2009, 17 (6), 763-771. DOI: 10.1111/j.1524-475X.2009.00543.x From NLM Medline.
3. Bellu, E.; Medici, S.; Coradduzza, D.; Cruciani, S.; Amler, E.; Maioli, M. Nanomaterials in Skin Regeneration and Rejuvenation. Int J Mol Sci 2021, 22 (13). DOI: 10.3390/ijms22137095 From NLM Medline.
4. Kirkwood, T. B.; Melov, S. On the programmed/non-programmed nature of ageing within the life history. Curr Biol 2011, 21 (18), R701-707. DOI: 10.1016/j.cub.2011.07.020 From NLM Medline.
5. Yurchenco, P. D.; Schittny, J. C. Molecular architecture of basement membranes. FASEB J 1990, 4 (6), 1577-1590. DOI: 10.1096/fasebj.4.6.2180767 From NLM Medline.
6. Bellu, E.; Garroni, G.; Balzano, F.; Satta, R.; Montesu, M. A.; Kralovic, M.; Fedacko, J.; Cruciani, S.; Maioli, M. Isolating stem cells from skin: designing a novel highly efficient non-enzymatic approach. Physiol Res 2019, 68 (Suppl 4), S385-S388. DOI: 10.33549/physiolres.934373 From NLM Medline.
7. Stadelmann, W. K.; Digenis, A. G.; Tobin, G. R. Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg 1998, 176 (2A Suppl), 26S-38S. DOI: 10.1016/s0002-9610(98)00183-4 From NLM Medline.
8. Braund, R.; Hook, S.; Medlicott, N. J. The role of topical growth factors in chronic wounds. Curr Drug Deliv 2007, 4 (3), 195-204. DOI: 10.2174/156720107781023857 From NLM Medline.
9. Gainza, G.; Villullas, S.; Pedraz, J. L.; Hernandez, R. M.; Igartua, M. Advances in drug delivery systems (DDSs) to release growth factors for wound healing and skin regeneration. Nanomedicine 2015, 11 (6), 1551-1573. DOI: 10.1016/j.nano.2015.03.002 From NLM Medline.
10. Addis, R.; Cruciani, S.; Santaniello, S.; Bellu, E.; Sarais, G.; Ventura, C.; Maioli, M.; Pintore, G. Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome. Int J Med Sci 2020, 17 (8), 1030-1042. DOI: 10.7150/ijms.43986 From NLM Medline.
11. Oda, Y.; Bikle, D. D. Vitamin D and calcium signaling in epidermal stem cells and their regeneration. World J Stem Cells 2020, 12 (7), 604-611. DOI: 10.4252/wjsc.v12.i7.604 From NLM PubMed-not-MEDLINE.
12. Maisel-Campbell, A. L.; Ismail, A.; Reynolds, K. A.; Poon, E.; Serrano, L.; Grushchak, S.; Farid, C.; West, D. P.; Alam, M. A systematic review of the safety and effectiveness of platelet-rich plasma (PRP) for skin aging. Arch Dermatol Res 2020, 312 (5), 301-315. DOI: 10.1007/s00403-019-01999-6 From NLM Medline.
13. Dhurat, R.; Sukesh, M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective. J Cutan Aesthet Surg 2014, 7 (4), 189-197. DOI: 10.4103/0974-2077.150734 From NLM PubMed-not-MEDLINE.
14. Miron, R. J.; Zucchelli, G.; Pikos, M. A.; Salama, M.; Lee, S.; Guillemette, V.; Fujioka-Kobayashi, M.; Bishara, M.; Zhang, Y.; Wang, H. L.; et al. Use of platelet-rich fibrin in regenerative dentistry: a systematic review. Clin Oral Investig 2017, 21 (6), 1913-1927. DOI: 10.1007/s00784-017-2133-z.
15. Dohan, D. M.; Choukroun, J.; Diss, A.; Dohan, S. L.; Dohan, A. J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006, 101 (3), e37-44. DOI: 10.1016/j.tripleo.2005.07.008.
16. Dohan, D. M.; Choukroun, J.; Diss, A.; Dohan, S. L.; Dohan, A. J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006, 101 (3), e45-50. DOI: 10.1016/j.tripleo.2005.07.009.
17. Gawdat, H. I.; Hegazy, R. A.; Fawzy, M. M.; Fathy, M. Autologous platelet rich plasma: topical versus intradermal after fractional ablative carbon dioxide laser treatment of atrophic acne scars. Dermatol Surg 2014, 40 (2), 152-161. DOI: 10.1111/dsu.12392 From NLM Medline.
18. Tuknayat, A.; Bhalla, M.; Thami, G. P. Platelet-rich plasma is a promising therapy for melasma. J Cosmet Dermatol 2021, 20 (8), 2431-2436. DOI: 10.1111/jocd.14229 From NLM Medline.
19. Na, J. I.; Choi, J. W.; Choi, H. R.; Jeong, J. B.; Park, K. C.; Youn, S. W.; Huh, C. H. Rapid healing and reduced erythema after ablative fractional carbon dioxide laser resurfacing combined with the application of autologous platelet-rich plasma. Dermatol Surg 2011, 37 (4), 463-468. DOI: 10.1111/j.1524-4725.2011.01916.x From NLM Medline.
20. Nacopoulos, C.; Vesala, A. M. Lower facial regeneration with a combination of platelet-rich fibrin liquid matrices based on the low speed centrifugation concept-Cleopatra technique. J Cosmet Dermatol 2020, 19 (1), 185-189. DOI: 10.1111/jocd.13196.
21. Sclafani, A. P.; Azzi, J. Platelet Preparations for Use in Facial Rejuvenation and Wound Healing: A Critical Review of Current Literature. Aesthetic Plast Surg 2015, 39 (4), 495-505. DOI: 10.1007/s00266-015-0504-x From NLM Medline.
22. Kim, E. K.; Kim, H. O.; Park, Y. M.; Park, C. J.; Yu, D. S.; Lee, J. Y. Prevalence and risk factors of depression in geriatric patients with dermatological diseases. Ann Dermatol 2013, 25 (3), 278-284. DOI: 10.5021/ad.2013.25.3.278 From NLM PubMed-not-MEDLINE.
23. Nacopoulos, C.; Gkouskou, K.; Karypidis, D.; Vlastos, I.; Vesala, A. M.; Choukroun, J.; Miron, R. J.; Prokopakis, E. Telomere length and genetic variations affecting telomere length as biomarkers for facial regeneration with platelet-rich fibrin based on the low-speed centrifugation concept. J Cosmet Dermatol 2019, 18 (1), 408-413. DOI: 10.1111/jocd.12666.
24. Trommer, H.; Neubert, R. H. Overcoming the stratum corneum: the modulation of skin penetration. A review. Skin Pharmacol Physiol 2006, 19 (2), 106-121. DOI: 10.1159/000091978 From NLM Medline.
25. Batisse, D.; Bazin, R.; Baldeweck, T.; Querleux, B.; Leveque, J. L. Influence of age on the wrinkling capacities of skin. Skin Res Technol 2002, 8 (3), 148-154. DOI: 10.1034/j.1600-0846.2002.10308.x From NLM Medline.
26. Dsouza, L.; Ghate, V. M.; Lewis, S. A. Derma rollers in therapy: the transition from cosmetics to transdermal drug delivery. Biomed Microdevices 2020, 22 (4), 77. DOI: 10.1007/s10544-020-00530-3.
27. Fernandes, D. Minimally invasive percutaneous collagen induction. Oral Maxillofac Surg Clin North Am 2005, 17 (1), 51-63, vi. DOI: 10.1016/j.coms.2004.09.004.
28. Singh, A.; Yadav, S. Microneedling: Advances and widening horizons. Indian Dermatol Online J 2016, 7 (4), 244-254. DOI: 10.4103/2229-5178.185468.
29. Vesala, A.-M.; Nacopoulos, C.; Gkouskou, K.; Amenta, F.; Ruga, E. Microneedling with injectable platelet-rich fibrin for facial rejuvenation. Plastic and Aesthetic Research 2021, 8 (0), 53.
30. Vesala, A.-M.; Nacopoulos, C.; Karypidis, D.; Ruga, E. Efficacy of microneedling with injectable platelet-rich fibrin (i-PRF) for facial skin rejuvenation. International Journal of Dermatology and Venereology 2023, Publish Ahead of Print, 10.1097/JD1099.0000000000000301. DOI: 10.1097/jd9.0000000000000301.
31. Krishnegowda, R.; Pradhan, S. N.; Belgaumkar, V. A. A Split-Face Study to Evaluate Efficacy of Autologous Injectable Platelet-Rich Fibrin With Microneedling Against Microneedling With Normal Saline (Placebo Control) in Atrophic Acne Scars. Dermatologic Surgery 2023, 49 (10), 938-942. DOI: 10.1097/dss.0000000000003893.
32. Wang, X.; Zhang, Y.; Choukroun, J.; Ghanaati, S.; Miron, R. J. Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP. Int J Mol Sci 2017, 18 (2). DOI: 10.3390/ijms18020331.
33. Miron, R. J.; Fujioka-Kobayashi, M.; Bishara, M.; Zhang, Y.; Hernandez, M.; Choukroun, J. Platelet-Rich Fibrin and Soft Tissue Wound Healing: A Systematic Review. Tissue Eng Part B Rev 2017, 23 (1), 83-99. DOI: 10.1089/ten.TEB.2016.0233.
34. Kubesch, A.; Barbeck, M.; Al-Maawi, S.; Orlowska, A.; Booms, P. F.; Sader, R. A.; Miron, R. J.; Kirkpatrick, C. J.; Choukroun, J.; Ghanaati, S. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo. Platelets 2019, 30 (3), 329-340. DOI: 10.1080/09537104.2018.1445835.
35. Wang, X.; Yang, Y.; Zhang, Y.; Miron, R. J. Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma. J Cosmet Dermatol 2019, 18 (6), 2004-2010. DOI: 10.1111/jocd.12955 From NLM Medline.
36. Choukroun, J.; Ghanaati, S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 2018, 44 (1), 87-95. DOI: 10.1007/s00068-017-0767-9.