Abstract
A novel and natural mesenchymal stem cell isolation procedure without the incorporation of any chemical or biological agents from fresh umbilical cord through cultural expansion and harvest is described, the resulting cells can be maintained up to 20 passages and exhibited MSC morphology and characteristics. Proficient and straight forward secretome complex was harvested from these stem cell cultures after cells reached genetic stability. The process was straightforward, sustainable and scalable; the secretome complex depicted reproducible clinical end-point evident disease-modifying activity in various clinical applications. These stem cell isolation processes exemplified a proficient and sustainable cell expansion protocol and the harvested secretome component that depicted clinical efficacy warrants further investigation.