Multiplexed bioluminescence reporter assay: Empowering screening strategy for future medicine
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Abstract
For past several decades dual luciferase reporter assay using non-overlapping firfly and Renilla luciferase formed the backbone of biomedical and biotechnology research applications. Though it was highly reliable, the application could essentially allow measuring only one gene function assessment at one go. Recent discovery of the mushroom luciferase (Luz) from Neonothopanus nambi has provided the opportunity for developing a non- overlapping, multiplex luciferase assay for simultaneous measurement of three or more gene functions from live cells. This development adds up strength by providing the scope for biomedical research applications dealing with complex gene function that is natural in a lot of normal or disease biology contexts.
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