Determination of Antifungal Susceptibility of Candida Species Isolated from Clinical Samples using Colorimetric Method

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Main Article Content

Iskender Karalti
Central Laboratory of Educational-Therapeutic Clinic, Azerbaijan Medical University, Baku, Azerbaijan.
Günay Tülay Colakoglu
Department of Biology, Faculty of Science, Marmara University Istanbul, Türkiye.
Zuhal Tekkanat Tazegun
Medical Microbiology, Laboratory Burdur State Hospital, Burdur, Türkiye.
Zehra Kipritci
Department of Medical Microbiology, Medical Faculty, Bahcesehir University, Istanbul Türkiye
Yeşim Gürol
Medical Microbiology Laboratory, Beykoz State Hospital, Istanbul, Türkiye.
Gülden Celik
Department of Medical Microbiology, Medical Faculty, Bahcesehir University, Istanbul Türkiye

Abstract

It was aimed to determine the antifungal susceptibility of Candida species isolated from patients by the colorimetric method, one of the standard modifications. Therefore ‘Sensititre YeastOne’ using an indicator alamar blue standard commercial kit was used.


The samples from patients applied to Yeditepe University Hospital Medical Microbiology Laboratory between years 2009-2011 were included to the study. 149 strains were isolated from 136 samplas. These strains were isolated from abscess, mouth swab, bronchoalveolar lavage (BAL), sputum, urine and urine catheter, blood culture, catheter, tracheal aspirate, sterile body fluid, vaginal swab and wound swab samples. Candida, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida dubliniensis, Candida krusei and, Candida incons./norveqen. of genus Candida were isolated.


Antifungal susceptibility tests were applied for seven antifungals including fluconazole, caspofungin, voriconazole, amphotericin B, posaconazole, itraconazole and 5- flusitosine. There was no resistant strain to caspofungin although the most resistant group was found in azole group antifungals. The resistance rates for fluconazole, voriconazole, itraconazole, 5- flusitosine, respectively were as 18.8%,13.4%,37.6%,2.7%. For amphotericin B and posaconazole minimum inhibition concentration (MIC) values because of in vivo-in vitro discrepancy, were excluded from the evaluation. It was performed by E TEST method, because of high fluconazole resistance rate and the same resistance rate was observed.


Because of easy evaluation, this test is found as a practical test for antifungal susceptibility.

Keywords: Candida, Colorimetric, Antifungal, Sensititre YeastOne

Article Details

How to Cite
KARALTI, Iskender et al. Determination of Antifungal Susceptibility of Candida Species Isolated from Clinical Samples using Colorimetric Method. Medical Research Archives, [S.l.], v. 13, n. 11, dec. 2025. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/7131>. Date accessed: 10 dec. 2025.
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Issue
Vol 13 No 11 (2025): Vol.13, Issue 11, November 2025
Section
Research Articles

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