In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.

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Mogens Claesson Anders Elm Pedersen


Enteroantigen specific B cells have previously been demonstrated to play an immunomodulatory role in experimental colitis such as the T cell transfer model of colitis and regulatory B cells are known to be important for suppressing immunopathology in human IBD. Establishing immortalized B cell hybridomas with enteroantigen specificity would be an important tool to investigate further the role of B cells in the immunopathology of experimental colitis and inflammatory bowel disease. Here, based on polyethylene glycol fusion of X63 cells and enteroantigen reactive murine CD19+ B cells, we generated enteroantigen specific B cell hybridomas with proliferative capacity and IL-10 secretion against enteroantigen. The establishment of such hybridomas might be the basis for further assessing the enteroantigen specificity of gut-derived B cells, enteroantigens relevant for experimental colitis as well as human IBD including the immunoregulatory role of such enteroantigen specific B cells.

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CLAESSON, Mogens; ELM PEDERSEN, Anders. In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.. Medical Research Archives, [S.l.], v. 5, n. Issue 9, sep. 2017. ISSN 2375-1924. Available at: <>. Date accessed: 07 oct. 2022.
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