In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.
Main Article Content
Abstract
Enteroantigen specific B cells have previously been demonstrated to play an immunomodulatory role in experimental colitis such as the T cell transfer model of colitis and regulatory B cells are known to be important for suppressing immunopathology in human IBD. Establishing immortalized B cell hybridomas with enteroantigen specificity would be an important tool to investigate further the role of B cells in the immunopathology of experimental colitis and inflammatory bowel disease. Here, based on polyethylene glycol fusion of X63 cells and enteroantigen reactive murine CD19+ B cells, we generated enteroantigen specific B cell hybridomas with proliferative capacity and IL-10 secretion against enteroantigen. The establishment of such hybridomas might be the basis for further assessing the enteroantigen specificity of gut-derived B cells, enteroantigens relevant for experimental colitis as well as human IBD including the immunoregulatory role of such enteroantigen specific B cells.
Article Details
How to Cite
CLAESSON, Mogens; ELM PEDERSEN, Anders.
In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody..
Medical Research Archives, [S.l.], v. 5, n. Issue 9, sep. 2017.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/1435>. Date accessed: 13 nov. 2024.
Section
Review Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
1. Berthelot, Jean-Marie; Jamin, Christophe; Amrouche, Kahina; Le Goff, Benoit; Maugars, Yves; Youinou, Pierre.2013 "Regulatory B cells play a key role in immune system balance". Joint Bone Spine 80 (1): 18–22. doi:10.1016/j.jbspin.2012.04.010. PMID 22858147. .[17] Next suppressive Breg mechanism is production of transforming growth factor (TGF-β), another anti-inflammatory cytokine.[13]
2. Vitale, G; Mion, F; Pucillo, C.2010 "Regulatory B cells: evidence, developmental origin and population diversity". Molecular immunology 48 (1–3): 1–8. doi:10.1016/j.molimm.2010.09.010. PMID 20950861.
3. Yanaba, K; Bouaziz, JD; Haas, KM; Poe, JC; Fujimoto, M; Tedder, TF.2008 "A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses.". Immunity 28 (5): 639–50. doi:10.1016/j.immuni.2008.03.017. PMID 18482568.
4. Iwata, Y; Matsushita, T; Horikawa, M; Dilillo, DJ; Yanaba, K; Venturi, GM; Szabolcs, PM; Bernstein, SH; Magro, CM; Williams, AD; Hall, RP; St Clair, EW; Tedder, TF.2011 "Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.". Blood 117 (2): 530–41. doi:10.1182/blood-2010-07-294249. PMID 20962324.
5. van de Veen, W; Stanic, B; Yaman, G; Wawrzyniak, M; Söllner, S; Akdis, DG; Rückert, B; Akdis, CA; Akdis, M.2013 "IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses". The Journal of allergy and clinical immunology 131 (4): 1204–12. doi:10.1016/j.jaci.2013.01.014. PMID 23453135.
6. Wolf, SD; Dittel, BN; Hardardottir, F; Janeway CA, Jr.1996 "Experimental autoimmune encephalomyelitis induction in genetically B cell-deficient mice.". The Journal of experimental medicine 184 (6): 2271–8. doi:10.1084/jem.184.6.2271. PMID 8976182. Janeway´s group observed an immunomodulation of experimental autoimmune encephalomyelitis (EAE) by B cells.[2]
7. Matsushita, Takashi; Yanaba, Koichi; Bouaziz, Jean-David; Fujimoto, Manabu; Tedder, Thomas F. 2008 "Regulatory B cells inhibit EAE initiation in mice while other B cells promote disease progression". Journal of Clinical Investigation. doi:10.1172/JCI36030.
8. Evans, JG; Chavez-Rueda, KA; Eddaoudi, A; Meyer-Bahlburg, A; Rawlings, DJ; Ehrenstein, MR; Mauri, C.2007 "Novel suppressive function of transitional 2 B cells in experimental arthritis.". Journal of immunology (Baltimore, Md. : 1950) 178 (12): 7868–78. PMID 17548625.
9. Mauri, C; Gray, D; Mushtaq, N; Londei, M.2003 "Prevention of arthritis by interleukin 10-producing B cells.". The Journal of experimental medicine 197 (4): 489–501.
10. Tian, J; Zekzer, D; Hanssen, L; Lu, Y; Olcott, A; Kaufman, DL.2001 "Lipopolysaccharide-activated B cells down-regulate Th1 immunity and prevent autoimmune diabetes in nonobese diabetic mice.". Journal of immunology (Baltimore, Md. : 1950) 167 (2): 1081–9. doi:10.4049/jimmunol.167.2.1081. PMID 11441119.
11. Mizoguchi, A; Mizoguchi, E; Smith, RN; Preffer, FI; Bhan, AK.1997 "Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice.". The Journal of experimental medicine 186 (10): 1749–56. doi:10.1084/jem.186.10.1749. PMID 9362534.
12. Asseman, C; Mauze, S; Leach, MW; Coffman, RL; Powrie, F.1999. "An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.". The Journal of experimental medicine 190 (7): 995–1004. doi:10.1084/jem.190.7.995. PMID 10510089.
13. El Fassi D, Nielsen CH, Kjeldsen J, Clemmensen O, Hegedüs L. 2008 Ulcerative colitis following B lymphocyte depletion with rituximab in a patient with Graves’ disease. Gut. May;57(5):714–5.
14. Goetz M, Atreya R, Ghalibafian M, Galle PR, Neurath MF. 2007 Exacerbation of ulcerative colitis after rituximab salvage therapy. Inflammatory bowel diseases. Nov;13(11):1365–8.
15. Ronet, C; Hauyon-La Torre, Y; Revaz-Breton, M; Mastelic, B; Tacchini-Cottier, F; Louis, J; Launois, P.2010. "Regulatory B cells shape the development of Th2 immune responses in BALB/c mice infected with Leishmania major through IL-10 production.". Journal of immunology (Baltimore, Md. : 1950) 184 (2): 886–94. doi:10.4049/jimmunol.0901114. PMID 19966209.
16. Douglas, RS; Woo, EY; Capocasale, RJ; Tarshis, AD; Nowell, PC; Moore, JS. 1997. "Altered response to and production of TGF-beta by B cells from autoimmune NZB mice.". Cellular immunology 179 (2): 126–37. doi:10.1006/cimm.1997.1149. PMID 9268496. or systemic lupus erythematosus (SLE).[5]
17. Lundy, SK; Boros, DL.2002. "Fas ligand-expressing B-1a lymphocytes mediate CD4(+)-T-cell apoptosis during schistosomal infection: induction by interleukin 4 (IL-4) and IL-10.". Infection and immunity 70 (2): 812–9. doi:10.1128/iai.70.2.812-819.2002. PMID 11796615.
18. Carter, Laura L.; Leach, Michael W.; Azoitei, Mihai L.; Cui, Junqing; Pelker, Jeffrey W.; Jussif, Jason; Benoit, Steve; Ireland, Gretchen; Luxenberg, Deborah; Askew, G. Roger; Milarski, Kim L.; Groves, Christopher; Brown, Tom; Carito, Brenda A.; Percival, Karen; Carreno, Beatriz M.; Collins, Mary; Marusic, Suzana.2007. "PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis". Journal of Neuroimmunology 182 (1–2): 124–134. doi:10.1016/j.jneuroim.2006.10.006. PMID 17182110.
19. Schmidt EG, Larsen HL, Kristensen NN, Poulsen SS, Claesson MH, Pedersen AE. 2012, B cells exposed to enterobacterial components suppress development of experimental colitis. Inflamm Bowel Dis Feb;18(2):284-93. doi: 10.1002/ibd.21769. Epub 2011 May 25. PubMed PMID: 21618359.
20. Venning FA, Trempenau ML, Schmidt E, Claesson MH. 2014 Enteroantigen (eAg)-binding B lymphocytes in the mouse - phenotype, distribution, function and eAg-specificantibody secretion. APMIS. Jul;122(7):616-27.
doi: 10.1111/apm.12200. Epub 2013 Dec 5.,
2. Vitale, G; Mion, F; Pucillo, C.2010 "Regulatory B cells: evidence, developmental origin and population diversity". Molecular immunology 48 (1–3): 1–8. doi:10.1016/j.molimm.2010.09.010. PMID 20950861.
3. Yanaba, K; Bouaziz, JD; Haas, KM; Poe, JC; Fujimoto, M; Tedder, TF.2008 "A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses.". Immunity 28 (5): 639–50. doi:10.1016/j.immuni.2008.03.017. PMID 18482568.
4. Iwata, Y; Matsushita, T; Horikawa, M; Dilillo, DJ; Yanaba, K; Venturi, GM; Szabolcs, PM; Bernstein, SH; Magro, CM; Williams, AD; Hall, RP; St Clair, EW; Tedder, TF.2011 "Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.". Blood 117 (2): 530–41. doi:10.1182/blood-2010-07-294249. PMID 20962324.
5. van de Veen, W; Stanic, B; Yaman, G; Wawrzyniak, M; Söllner, S; Akdis, DG; Rückert, B; Akdis, CA; Akdis, M.2013 "IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses". The Journal of allergy and clinical immunology 131 (4): 1204–12. doi:10.1016/j.jaci.2013.01.014. PMID 23453135.
6. Wolf, SD; Dittel, BN; Hardardottir, F; Janeway CA, Jr.1996 "Experimental autoimmune encephalomyelitis induction in genetically B cell-deficient mice.". The Journal of experimental medicine 184 (6): 2271–8. doi:10.1084/jem.184.6.2271. PMID 8976182. Janeway´s group observed an immunomodulation of experimental autoimmune encephalomyelitis (EAE) by B cells.[2]
7. Matsushita, Takashi; Yanaba, Koichi; Bouaziz, Jean-David; Fujimoto, Manabu; Tedder, Thomas F. 2008 "Regulatory B cells inhibit EAE initiation in mice while other B cells promote disease progression". Journal of Clinical Investigation. doi:10.1172/JCI36030.
8. Evans, JG; Chavez-Rueda, KA; Eddaoudi, A; Meyer-Bahlburg, A; Rawlings, DJ; Ehrenstein, MR; Mauri, C.2007 "Novel suppressive function of transitional 2 B cells in experimental arthritis.". Journal of immunology (Baltimore, Md. : 1950) 178 (12): 7868–78. PMID 17548625.
9. Mauri, C; Gray, D; Mushtaq, N; Londei, M.2003 "Prevention of arthritis by interleukin 10-producing B cells.". The Journal of experimental medicine 197 (4): 489–501.
10. Tian, J; Zekzer, D; Hanssen, L; Lu, Y; Olcott, A; Kaufman, DL.2001 "Lipopolysaccharide-activated B cells down-regulate Th1 immunity and prevent autoimmune diabetes in nonobese diabetic mice.". Journal of immunology (Baltimore, Md. : 1950) 167 (2): 1081–9. doi:10.4049/jimmunol.167.2.1081. PMID 11441119.
11. Mizoguchi, A; Mizoguchi, E; Smith, RN; Preffer, FI; Bhan, AK.1997 "Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice.". The Journal of experimental medicine 186 (10): 1749–56. doi:10.1084/jem.186.10.1749. PMID 9362534.
12. Asseman, C; Mauze, S; Leach, MW; Coffman, RL; Powrie, F.1999. "An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.". The Journal of experimental medicine 190 (7): 995–1004. doi:10.1084/jem.190.7.995. PMID 10510089.
13. El Fassi D, Nielsen CH, Kjeldsen J, Clemmensen O, Hegedüs L. 2008 Ulcerative colitis following B lymphocyte depletion with rituximab in a patient with Graves’ disease. Gut. May;57(5):714–5.
14. Goetz M, Atreya R, Ghalibafian M, Galle PR, Neurath MF. 2007 Exacerbation of ulcerative colitis after rituximab salvage therapy. Inflammatory bowel diseases. Nov;13(11):1365–8.
15. Ronet, C; Hauyon-La Torre, Y; Revaz-Breton, M; Mastelic, B; Tacchini-Cottier, F; Louis, J; Launois, P.2010. "Regulatory B cells shape the development of Th2 immune responses in BALB/c mice infected with Leishmania major through IL-10 production.". Journal of immunology (Baltimore, Md. : 1950) 184 (2): 886–94. doi:10.4049/jimmunol.0901114. PMID 19966209.
16. Douglas, RS; Woo, EY; Capocasale, RJ; Tarshis, AD; Nowell, PC; Moore, JS. 1997. "Altered response to and production of TGF-beta by B cells from autoimmune NZB mice.". Cellular immunology 179 (2): 126–37. doi:10.1006/cimm.1997.1149. PMID 9268496. or systemic lupus erythematosus (SLE).[5]
17. Lundy, SK; Boros, DL.2002. "Fas ligand-expressing B-1a lymphocytes mediate CD4(+)-T-cell apoptosis during schistosomal infection: induction by interleukin 4 (IL-4) and IL-10.". Infection and immunity 70 (2): 812–9. doi:10.1128/iai.70.2.812-819.2002. PMID 11796615.
18. Carter, Laura L.; Leach, Michael W.; Azoitei, Mihai L.; Cui, Junqing; Pelker, Jeffrey W.; Jussif, Jason; Benoit, Steve; Ireland, Gretchen; Luxenberg, Deborah; Askew, G. Roger; Milarski, Kim L.; Groves, Christopher; Brown, Tom; Carito, Brenda A.; Percival, Karen; Carreno, Beatriz M.; Collins, Mary; Marusic, Suzana.2007. "PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis". Journal of Neuroimmunology 182 (1–2): 124–134. doi:10.1016/j.jneuroim.2006.10.006. PMID 17182110.
19. Schmidt EG, Larsen HL, Kristensen NN, Poulsen SS, Claesson MH, Pedersen AE. 2012, B cells exposed to enterobacterial components suppress development of experimental colitis. Inflamm Bowel Dis Feb;18(2):284-93. doi: 10.1002/ibd.21769. Epub 2011 May 25. PubMed PMID: 21618359.
20. Venning FA, Trempenau ML, Schmidt E, Claesson MH. 2014 Enteroantigen (eAg)-binding B lymphocytes in the mouse - phenotype, distribution, function and eAg-specificantibody secretion. APMIS. Jul;122(7):616-27.
doi: 10.1111/apm.12200. Epub 2013 Dec 5.,