In-vitro Antioxidant and Radical Scavenging Properties of Acacia Ferruginea Plant Stem: A Phytochemical Study
Main Article Content
Abstract
Introduction: The study of free radical chemistry has received considerable attention in recent years. Our bodies produce free radicals through a variety of endogenous systems, exposed to various physiochemical or pathological conditions.
Objective: The study aimed to assess the in-vitro antioxidant properties of Acacia ferruginea plant stem and to screen for their phytochemical components.
Methodology: The stem was examined for enzymatic and non-enzymatic antioxidants, radical scavenging activity in three solvents with varying polarities, and phytochemical analyses.
Result: The stem of Acacia ferruginea possessed considerable levels of enzymatic and non-enzymatic antioxidants.
Conclusion: Although the three extracts had high scavenging properties, in-vitro assays did not show any significant DNA preventive properties. Phytochemical screening indicated the presence of alkaloids, flavonoids and phenols.
Article Details
How to Cite
RAMYA, Nagesh; BALASUBRAMANIAN, Kiruthika; HARISH, -.
In-vitro Antioxidant and Radical Scavenging Properties of Acacia Ferruginea Plant Stem: A Phytochemical Study.
Medical Research Archives, [S.l.], v. 11, n. 11, nov. 2023.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/4714>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.18103/mra.v11i11.4714.
Section
Research Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
References
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32. Scartezzini P, Speroni E. Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol. 2000;71(1-2):23-43.
33. Karthikeyan J, Rani P. Enzymatic and non-enzymatic antioxidants in selected Piper species. Indian J Exp Biol. 2003;41(2):135-140.
34. Vanacker H, Carver TL, Foyer CH. Early H(2)O(2) accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barley-powdery mildew interaction. Plant Physiol. 2000;123(4):1289-1300.
35. Quan LJ, Zhang B, Shi WW, Li HY. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol. 2008;50(1):2-18.
36. Hung S-H, Yu C-W, Lin CH. Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin. 2005;46:1–10.
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38. Munné-Bosch S. The role of alpha-tocopherol in plant stress tolerance. J Plant Physiol. 2005;162(7):743-748.
39. Arora R, Gupta D, Chawla R, et al. Radioprotection by plant products: present status and future prospects. Phytother Res. 2005;19(1):1-22.
40. Tharakan B, Dhanasekaran M, Manyam BV. Antioxidant and DNA protecting properties of anti-fatigue herb Trichopus zeylanicus. Phytother Res. 2005;19(8):669-673.
2. Nair R and Chanda SV. Antibacterial activities of some medicinal plants of the western region of India. Turk. J. Biol. 2007; 31: 231-236.
3. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A. 1993;90(17):7915-7922.
4. Ahmad P, Sarwat M, Sharma S. Reactive oxygen species, antioxidants and signaling in plants. J. Plant Biol. 2008;51:167-173.
5. Rahmatullah M, Jahan R, Khatun MA, Jahan F, Azad AK, Bashar AA, Miajee ZE, Ahsan SN, Ahmad NI, Chowdhury MH. A pharmacological evaluation of medicinal plants used by folk medicinal practitioners of Station Purbo Para village of Jamalpur Sadar Upazila in Jamalpur district, Bangladesh. Am.-Eurasian J. Sustain. Agric. 2010; 4: 170-195.
6. El-Missiry MA, Fayed TA, El-Sawy MR, El-Sayed AA. Ameliorative effect of melatonin against gamma-irradiation-induced oxidative stress and tissue injury. Ecotoxicol Environ Saf. 2007;66(2):278-286.
7. Hadjigogos K. The role of free radicals in the pathogenesis of rheumatoid arthritis. Panminerva Med. 2003;45(1):7-13.
8. Naik AK, Tandan SK, Dudhgaonkar SP, et al. Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats. Eur J Pain. 2006;10(7):573-579.
9. Buonocore G, Groenendaal F. Anti-oxidant strategies. Semin Fetal Neonatal Med. 2007;12(4):287-295.
10. Ramachandra Reddy A, Chaitanya KV, Vivekanandan M. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol. 2004;161(11):1189-1202.
11. Quan LJ, Zhang B, Shi WW, Li HY. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol. 2008;50(1):2-18.
12. Vijayakumar M, Govindarajan R, Rao GM, et al. Action of Hygrophila auriculata against streptozotocin-induced oxidative stress. J Ethnopharmacol. 2006;104(3):356-361.
13. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-582
14. Luck H. Catalase. In: Bergmeyer H.W., editor. Methods of Enzymatic Analysis, section 3. Academic Press; New York, NY, USA: 1963. pp. 885–894.
15. Reddy KP, Subhani SM, Khan PA, Kumar KB. Effect of light and benzyl adenine and dark treated graving rice (Oryza sativa) leaves changes in peroxidases activity. Plant Cell Physiol. 1995.26;987-994.
16. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974;249(22):7130-7139.
17. Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through a 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J Biol Chlem. 1943;147:399-407.
18. Rosenberg HR. Chemistry and physiology of the vitamins. New York: Interscience Publishers; 1992. pp. 452–453.
19. Zakaria M, Simpson K, Brown PR, Krstulovic A. Use of reversed-phase high-performance liquid chromatographic analysis for the determination of provitamin A carotenes in tomatoes. J Chromatogr. 1979;176(1):109-117.
20. Mallick CP, Singh MB, Plant enzymology and plant histoenzymology, Kalaya. Pub. New Delhi. 1980. 281- 286.
21. Cameron GR, Mitton RF and Allan JW. Measurements of flavonoids in plant sample. Lancet. 1943. 179.
22. Witham FH Devlin RM Blaydes DF. Experiments in Plant Physiology. New York: Van Nostrand Reinhold; 1971.
23. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;582(1):67-78.
24. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10(6):1003-1008.
25. Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharmaceut. 1990;58:237–240.
26. Brown DJ, Goodman J. A review of vitamins A, C, and E and their relationship to cardiovascular disease. Clin Excell Nurse Pract. 1998;2(1):10-22.
27. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 1961. 3:208-218.
28. Khandelwal KR. Practical Pharmacognosy Techniques & Experiments. Edition 19th, Nirali Prakashan, Pune. 2008. pp. 146, 149-161.
29. Lai LS, Chou ST, Chao WW. Studies on the antioxidative activities of Hsian-tsao (Mesona procumbens Hemsl) leaf gum. J Agric Food Chem. 2001;49(2):963-968.
30. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-126.
31. Sakthivel KM, Guruvayoorappan C. Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2. J Immunotoxicol. 2016;13(1):127-135.
32. Scartezzini P, Speroni E. Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol. 2000;71(1-2):23-43.
33. Karthikeyan J, Rani P. Enzymatic and non-enzymatic antioxidants in selected Piper species. Indian J Exp Biol. 2003;41(2):135-140.
34. Vanacker H, Carver TL, Foyer CH. Early H(2)O(2) accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barley-powdery mildew interaction. Plant Physiol. 2000;123(4):1289-1300.
35. Quan LJ, Zhang B, Shi WW, Li HY. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol. 2008;50(1):2-18.
36. Hung S-H, Yu C-W, Lin CH. Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin. 2005;46:1–10.
37. Sroka Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol. 2003;41(6):753-758.
38. Munné-Bosch S. The role of alpha-tocopherol in plant stress tolerance. J Plant Physiol. 2005;162(7):743-748.
39. Arora R, Gupta D, Chawla R, et al. Radioprotection by plant products: present status and future prospects. Phytother Res. 2005;19(1):1-22.
40. Tharakan B, Dhanasekaran M, Manyam BV. Antioxidant and DNA protecting properties of anti-fatigue herb Trichopus zeylanicus. Phytother Res. 2005;19(8):669-673.