Abstract
Studies from sub-Saharan Africa where malaria is endemic have observed high incidences of Glucose-6-phosphate dehydrogenase A- (G-6-PD A-) deficiency that sporadically leads to manifest haemolytic anaemia. The severity of this disease depends on the genetic variant involved, thus, pinpointing the type of G-6-PD variants will help in the management of G-6-PD deficiency. This is not exactly known among Hausa and Fulani ethnics in Northern Nigeria, hence the need for the study. Two ml of venous blood samples were collected from twenty (20) G-6-PD deficient individuals and dispensed into EDTA bottle to study the coding region of G-6-PD gene by using PCR-Sequencing techniques. Sequencing of the G-6-PD-deficient samples revealed two major polymorphic variants; A376G substitution (exon-5: Asn126Asp) and A376G/G202A substitution (exon-4: Val68Met) commonly observed among Hausa and Fulani ethnics. This is the first study of G-6-PD mutations in Kano, North-west Nigeria. The G-6-PD deficiency in Hausa and Fulani ethnics were associated with African variants (202A/376G G6PD A- allele) in exon 4 and no mutation was found within exon 6-7.