Abstract
Aim: To histologically study bone regeneration in cases of open wound healing
Material and method: In a case series of 20 patients, teeth which were fractured/ failed root canal which needed extraction and implant placement were included in the study. In all the cases, minimally invasive tooth extraction was done and care was taken not to damage either of the bony walls. Maxresorb bone graft (Botiss, Germany) was placed in the socket. A single layer of collagen membrane, Ossix plus membrane (Dentsply Sirona, Germany) was placed on the bone graft and 3-0 silk sutures were placed. After 5 months bone core was taken for histological analysis and Mis implant 3.75/11.5 implant was placed at the same site.
For histopathology, neutralized buffered formalin solution was used to fix the 2 mm trephine core biopsies and the soft tissue they overlaid right away, lasting between 24 and 48 hours. After which, the normal laboratory approach for tissue processing involving dehydration, cleaning, and paraffin wax infiltration was conducted for both the tissues. Hematoxylin and Eosin stains were used on sections that were 3 microns thick. Van Gieson, Massons trichrome and Picrosirus red were performed.
Results: soft tissue showed long thin rete ridges of parakeratinizd startified suamous epithelium and underlying dense collagenous stroma with few inflammatory cells chiefly lymphocytes. Bony tissue displayed well-formed regions of bony trabeculae with various mineralization phases.
Conclusion: open wound healing leads to bony regeneration as one can predict in closed healing.